Treatment of lateral epicondylitis using skin-derived tenocyte-like cells.

OBJECTIVES: To culture collagen-producing cells derived from skin fibroblasts and o evaluate prospectively the safety and potential use of this cell preparation for treatment of refractory lateral epicondylitis in a pilot study. DESIGN: Prospective clinical pilot study. SETTING: Institution-based clinical research. PATIENTS: A total of 12 patients (5 men and 7 women; mean age 39.1 years) with clinical diagnosis of refractory lateral epicondylitis. INTERVENTIONS: Laboratory-prepared collagen-producing cells derived from dermal fibroblasts were injected into the sites of intrasubstance tears and fibrillar discontinuity of the common extensor origin under ultrasonography guidance. MAIN OUTCOME MEASURES: The outcome assessment was performed over 6 months. The Patient-Rated Tennis Elbow Evaluation (PRTEE) scale was used to assess pain severity and functional disability. Tendon healing response was measured using four criteria on ultrasonography: tendon thickness, hypoechogenicity, intrasubstance tears and neovascularity. RESULTS: Cell cultures rich in collagen-producing cells was successfully prepared. After injection, the median PRTEE score decreased from 78 before the procedure to 47 at 6 weeks, 35 at 3 months and 12 at 6 months after the procedure (p<0.05). The healing response on ultrasonography showed median decrease in: (1) number of tears, from 5 to 2; (2) number of new vessels, from 3 to 1; and (3) tendon thickness, from 4.35 to 4.2 (p<0.05). Of the 12 patients, 11 had a satisfactory outcome, and only one patient proceeded to surgery after failure of treatment at the end of 3 months. CONCLUSIONS: Skin-derived tenocyte-like cells can be cultured in the laboratory to yield a rich preparation of collagen-producing cells. Our pilot study suggests that these collagen-producing cells can be injected safely into patients and may have therapeutic value in patients with refractory lateral epicondylitis.

Upright positional MRI of the lumbar spine.

Supine magnetic resonance imaging (MRI) is routinely used in the assessment of low back pain and radiculopathy. However, imaging findings often correlate poorly with clinical findings. This is partly related to the positional dependence of spinal stenosis, which reflects dynamic changes in soft-tissue structures (ligaments, disc, dural sac, epidural fat, and nerve roots). Upright MRI in the flexed, extended, rotated, standing, and bending positions, allows patients to reproduce the positions that bring about their symptoms and may uncover MRI findings that were not visible with routine supine imaging. Assessment of the degree of spinal stability in the degenerate and postoperative lumbar spine is also possible. The aim of this review was to present the current literature concerning both the normal and symptomatic spine as imaged using upright MRI and to illustrate the above findings using clinical examples.

Incidental vertebral fractures on multidetector CT images of the chest: prevalence and recognition.

AIM: To investigate the prevalence, demographics, fracture site, and the rate of vertebral fracture recognition by radiologists on multidetector computed tomography (MDCT) images of the chest. METHOD: The images of 200 consecutive patients who underwent a MDCT examination of the chest over a 4-month period were reviewed. The thoracic spine, viewed using bone windows in sagittal reformats, was reviewed in consensus by three radiologists. Vertebral fractures were assessed using a validated semi-quantitative method. RESULTS: The mean age was 61 years (range 18-92 years); 48% were female. There were 70 (35%) fractures, of which 51 (73%) were mild grade 1 fractures, 13 (19%) moderate grade 2 fractures, and six (9%) severe grade 3 fractures. Fractures commonly affected patients above the age of 50. The most common site was in the lower thoracic region (53%). The overall recognition rate on the radiologists' reports was 6 (9%). CONCLUSIONS: One in three patients who underwent MDCT of the chest had vertebral fractures. There is significant underreporting of these fractures, and the importance of fracture identification should be emphasized to avoid under-diagnosis.

Prevalence and diagnostic significance of fluid-fluid levels in soft-tissue neoplasms.

AIM: To report the prevalence of fluid-fluid levels (FFLs) in soft-tissue tumours as demonstrated by magnetic resonance imaging (MRI) and the potential diagnostic relevance of this finding. MATERIALS AND METHODS: A retrospective analysis was performed of 726 consecutive patients (361 women, 365 men, mean age 47.6 years+/-20.1 SD) presenting with a soft-tissue mass over a 7-year period. All subjects underwent MRI and final diagnosis was based on biopsy/surgical resection, or clinical follow-up and characteristic imaging findings. The patients were divided according to the presence or absence of FFLs on T2-weighted (T2W) axial MRI and histological diagnosis (non-neoplastic, neoplastic benign, neoplastic malignant). Cases with FFLs were sub-categorized depending upon the proportion of tumour containing FFLs: <1/3, 1/3-2/3 and >2/3, in order to determine whether the proportion of FFLs was useful for differential diagnosis. RESULTS: Twenty-four of the 726 (3.3%: confidence interval 2.1-4.9%) soft-tissue masses contained FFLs. One of the 24 (4.1%) was non-neoplastic (one ganglion), 12 (50.0%) were benign neoplasms (nine haemangiomas, two schwannomas, one hamartoma) and 11 (45.9%) were malignant neoplasms (one leiomyosarcoma, one liposarcoma, one malignant fibrous histocytoma, one mxyofibrosarcoma, two primitive neuroectodermal tumours, two synovial sarcomas, one spindle cell sarcoma, and two sarcomas not otherwise specified). The presence of FFLs did not help to differentiate benign from malignant neoplasms. Of the 12 benign neoplasms, 66.7% contained over two-thirds FFLs, the majority of which were haemangiomas. Of the 10 malignant neoplasms, all contained less than two-thirds FFLs: 20% had less than one-third, 80% had one to two-thirds FFLs. CONCLUSIONS: The prevalence of FFLs in soft-tissue tumours referred to a specialist orthopaedic oncology unit is 3.3%. However, the presence of FFLs does not reliably distinguish benign from malignant neoplasms, although all lesions with more than two-thirds FFLs were benign.

MRI findings in the lumbar spines of asymptomatic, adolescent, elite tennis players.

OBJECTIVE: To describe magnetic resonance imaging (MRI) findings in the lumbar spine in asymptomatic elite adolescent tennis players, to serve as the baseline for a future prospective longitudinal cohort study. DESIGN: Observational study. SETTING: Institutional, national tennis centre. PARTICIPANTS: 33 asymptomatic elite adolescent tennis players, mean (SD) age, 17.3 (1.7) years (18 male, 15 female). METHODS: Sagittal T1, T2, STIR, and axial T2 weighted MRI images were reviewed for the presence of abnormalities by two radiologists in consensus. Abnormalities included disc degeneration, disc herniation, pars lesions (fracture or stress reaction), and facet joint arthropathy. RESULTS: Five players (15.2%) had a normal MRI examination and 28 (84.8%) had an abnormal examination. Nine players showed pars lesions (10 lesions; one at two levels) predominately at the L5 level (9/10, L5; 1/10, L4). Three of the 10 lesions were complete fractures; two showed grade 1 and one grade 2 spondylolisthesis, both of which resulted in moderate narrowing of the L5 exit foramen. There were two acute and five chronic stress reactions of the pars. Twenty three patients showed signs of early facet arthropathy occurring at L5/S1 (15/29 joints) and L4/5 (12/29 joints). These were classified as mild degeneration (20/29) and moderate degeneration (9/29), with 20/29 showing sclerosis and 24/29 showing hypertrophy of the facet joint. Synovial cysts were identified in 14 of the 29 joints. Thirteen players showed disc desiccation and disc bulging (mild in 13; moderate in two) most often at L4/5 and L5/S1 levels (12 of 15 discs). CONCLUSIONS: Abnormalities were frequent, predominately in the lower lumbar spine, almost exclusively at L4/5 and L5/S1 levels. Pars injuries and facet joint arthroses were relatively common.

The role of MR imaging in the diagnostic characterisation of appendicular bone tumours and tumour-like conditions.

MRI has an established role in the local staging of primary bone tumours. However, as the majority of tumours have non-specific appearances on MRI, the diagnosis is usually established on the basis of clinical history, plain film findings and biopsy. This article reviews the value of MRI in the further characterisation of appendicular bone tumours and tumour-like lesions, with particular reference to peri-lesional oedema, fluid-fluid levels, flow voids, fat signal, cartilage signal and dedifferentiation. These features are a useful adjunct for distinguishing between benign and malignant disease, pointing towards a more specific diagnosis, and guiding biopsy.

Diseases of the submandibular gland as demonstrated using high resolution ultrasound.

In this review the high resolution ultrasound appearances of the normal anatomy and pathology involving the submandibular gland are demonstrated. The submandibular gland is affected by a number of disease processes that may be difficult to distinguish clinically. Its superficial location makes it ideal for ultrasound evaluation and a useful adjunct to clinical examination. In the assessment of submandibular pathology ultrasound allows intraglandular and extraglandular lesions to be localized and differentiated. These lesions can be further characterized as being benign or malignant and the extent of any extraglandular extension determined. Ultrasound is the first-line investigation in the assessment of sialolithiasis due to its high specificity and sensitivity. In severe infective sialadenitis ultrasound can confirm the presence and guide drainage of abscesses. Ultrasound also has an important role in the assessment of chronic inflammatory disorders such as sarcoidosis and Sjogren's syndrome.

Sonographic assessment of the submandibular space.

There is a wide variety of pathological processes which may present with swelling in the submandibular space. Although the submandibular gland is the most important structure in this region, there are a number of extraglandular causes of swelling which frequently mimic submandibular gland enlargement. In this review the use of high-resolution ultrasound in the assessment of the submandibular gland and adjacent structures is discussed and illustrated.