Removal of nitrogen from wastewater of date processing industries using a Saudi Arabian mesophilic bacterium, Stenotrophomonas maltophilia Al-Dhabi-17 in sequencing batch reactor.

The main aim of the present study was to assess the technical feasibility of nutrients removal from the wastewater from the date processing industries in sequencing batch reactor. Heterotrophic nitrifying and aerobic denitrifying bacteria were isolated from the soil sediment samples. The bacterial strain Al-Dhabi-17 effectively removed nutrients than other isolates from the wastewater and characterized as Stenotrophomonas maltophilia Al-Dhabi-17. The nutrient removal efficacy was improved by optimizing process parameters. Removal of NH4+ from the medium reached 42% within 60 h of cultivation and the nitrification rate was 111 ± 3.1 mg after 24 h. After 96 h, NO3- reached 6 ± 0.4 mg/mL concentration. The strain S. maltophilia Al-Dhabi-17 showed the ability to utilize NH4+ ranged between 100 and 300 mg/L. The supplemented sucrose, glucose and date molasses reached maximum nitrification process after 72 h (p < 0.05). Reduction of NH4+ -N reached 73.4% within 48 h time in the medium supplemented with date molasses. Nutrient removal was observed in the broad pH range (6.0-8.5) and maximum nutrient removal achieved at alkaline range (p < 0.05). Sequencing batch reactor was fed with wastewater and nutrient removal was analyzed under optimized condition. The associated chemical oxygen demand, phosphate and total nitrogen removal efficiencies for the suspended growth sequencing batch reactor were 96.5%, 97.9% and 88.4%, respectively. The sequencing batch reactor inoculated with S. maltophilia Al-Dhabi-17 showed promising for nitrogen removal.

Nephroprotective effect of pigmented violacein isolated from Chromobacterium violaceum in wistar rats.

The present study aimed to analyze the nephroprotective property of violacein obtained from the bacterium, Chromobacterium violaceum. The nephrotoxicity in the animal model was induced by gentamicin, potassium dichromate, mercuric chloride, and cadmium chloride-induced nephrotoxicity in the Wistar rats was analyzed by measuring the serum creatinine, uric acid, and urea level. The present investigation revealed the nephroprotective property on convoluted proximal tubule (S1 and S2 segments) and the straight proximal tubule (S3 segment). Also, violacein significantly improved the renal function by the renal protective property on S2 segment of proximal tubule from the nephrotoxicity stimulated by mercuric chloride, potassium dichromate, cadmium chloride and gentamicin in animal models. Animal model studies revealed that violacein at 20 and 40 mg/kg p.o improved the renal function and significantly reduced the increased amount of uric acid, creatinine, and blood urea compared to the control.