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1.
Biochemistry (Mosc) ; 63(2): 207-11, 1998 Feb.
Article in English | MEDLINE | ID: mdl-9526116

ABSTRACT

The preparation of site-specific endonuclease Bsp123 I was isolated and purified from the thermophilic strain Bacillus species 123. Endonuclease Bsp123 I recognizes the sequence CGCG and cleaves it in the middle between nucleotides G and C, producing blunt ends. Thus, Bsp123 I is an isoschizomer of FnuDII. The maximal activity of the enzyme is displayed at 42 degrees C, pH 8.0, 100 mM NaCl, 10 mM MgCl2.


Subject(s)
Bacillus/enzymology , Deoxyribonucleases, Type II Site-Specific/isolation & purification , DNA, Viral/metabolism , Deoxyribonucleases, Type II Site-Specific/metabolism , Substrate Specificity
2.
Biochemistry (Mosc) ; 63(5): 537-45, 1998 May.
Article in English | MEDLINE | ID: mdl-9632889

ABSTRACT

Thermophilic strain Bacillus species AA contains several site-specific endonucleases and three of them have been identified. BspAAI recognizes the sequence 5'-C downward arrow TCGAG-3' and cleaves it after the first "C" forming 4-nucleotide 5'-ends and is an isoschizomer of XhoI. BspAAII recognizes the sequence 5'-T downward arrow CTAGA-3' and cleaves it after the first "T" forming 4-nucleotide 5'-ends and is an isoschizomer of XbaI. BspAAIII recognizes the sequence 5'-GGATCC-3' and is an isomer of BamHI. The optimal temperature and pH values are 42-48 degreesC and 7.0-8.0, respectively.


Subject(s)
Bacillus/enzymology , Deoxyribonucleases, Type II Site-Specific/metabolism , Bacteriophage lambda/genetics , Bacteriophage lambda/metabolism , Base Sequence , Binding Sites , DNA, Viral/genetics , DNA, Viral/metabolism , Deoxyribonuclease BamHI/isolation & purification , Deoxyribonuclease BamHI/metabolism , Deoxyribonucleases, Type II Site-Specific/isolation & purification , Hydrogen-Ion Concentration , Substrate Specificity , Temperature
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