ABSTRACT
<b>Background and Objective:</b> Control of the stored date insects by an eco-friendly method is essential to maintain the fruit for prolonged periods, especially that dates are eaten fresh. The search for a safe method, as an alternative to commonly used chemical pesticides, is needed. The objective is to investigate CO<sub>2</sub> enriched atmosphere on the Mortality Percentage (MP) of the Saw-toothed grain beetle, <i>O. surinamensis</i>, life stages with special emphasis on reducing damage to stored dates. <b>Materials and Methods:</b> The effect of high levels of CO<sub>2</sub> as an alternative control method against the Saw-toothed grain beetle, <i>Oryzaephilus surinamensis</i> L., at different life stages was studied on infested 'Saqie' dates. Four CO<sub>2</sub> pressures (25, 50, 75 and 90 kPa, balance is nitrogen) were tested for 6, 12, 18, 24, 48, 72 and 96 hrs intervals. The response of different life stages of <i>O. surinamensis</i> to the different treatments varied according to CO<sub>2</sub> level, developmental stage and exposure period. <b>Results:</b> Mortality (%) was higher during the larval stage, followed by adults, pupae and eggs, in descending order. The larvae and adult stages were more sensitive to CO<sub>2</sub> treatment than the pupal and egg. Exposure time was more effective on eggs, larval and adult MP than the CO<sub>2</sub> atmosphere level. Mortality% at 96 hrs exposure time was almost 100% with CO<sub>2</sub> atmospheres of 50, 75 and 90 kPa. Mathematical equations were developed to model the relationship between mortality% and CO<sub>2</sub> treatments using multiple regression analyses for each life stage. <b>Conclusion:</b> The results confirmed that CO<sub>2</sub> could be applied to final food products during packaging to control the residual occurrence of insect pests after storage and before the packaging process to prevent further infestation in the final packages.