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1.
Trop Anim Health Prod ; 51(5): 1239-1246, 2019 Jun.
Article in English | MEDLINE | ID: mdl-30694430

ABSTRACT

Colostrum is crucial for the survival and growth of suckling piglets. However, both the quantity and quality of colostrum are highly variable among sows. The aim of the present study was to determine the impact of sow parity number and housing conditions on concentration of immunoglobulin G in sow colostrum. A total of 358 colostrum samples were collected from two commercial swine herds in Thailand. The colostrum samples were collected from all teats at 1 and 6 h after the onset of farrowing and kept at - 20 °C until analysis. The concentration of IgG was determined using ELISA. The concentration of IgG in colostrum at 1 h after the onset of farrowing was greater than the concentration of IgG at 6 h after the onset of farrowing (P < 0.001). Moreover, herd A had a greater colostral IgG concentration than herd B (P < 0.001). The concentration of IgG in primiparous sows (64.0 mg/ml) was lower than that in sow parity numbers 3 (75.1 mg/ml, P = 0.05) and 6 (79.2 mg/ml, P = 0.04). In conclusion, the variation in colostral immunoglobulin concentration in the sow colostrum was influenced by their parity number and housing conditions. The concentration of IgG declined significantly within 6 h after the onset of farrowing (P < 0.001).


Subject(s)
Colostrum/chemistry , Housing, Animal , Immunoglobulin G/chemistry , Parity , Swine/metabolism , Animal Husbandry , Animals , Enzyme-Linked Immunosorbent Assay , Female , Immunoglobulin G/metabolism , Pregnancy
2.
J Dairy Sci ; 94(7): 3467-76, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21700033

ABSTRACT

The objective of these experiments was to test the hypothesis that transcript abundance of cationic AA transporter- and milk protein-encoding genes increase in the porcine mammary gland in response to higher lactation demand. Genes of interest included those encoding for the milk proteins α-lactalbumin (α-LA) and ß-casein (ß-CN; LALBA and CSN2, respectively), and AA transporter b(0,+)AT, y(+)LAT1, y(+)LAT2, ATB(0,+), CAT-1, and CAT-2b (SLC7A9, SLC7A7, SLC7A6, SLC6A14, SLC7A1, and SLC7A2, respectively). Mammary tissue was biopsied from 4 sows on d 110 of gestation (prepartum), on d 2 (early postpartum), on d 5 (early), and d 17 (peak) of lactation, and on d 5 after weaning (postweaning), and mRNA of target genes quantified by reverse transcription quantitative PCR. Compared with prepartum, CAT-1, ATB(0,+), y(+)LAT2, ß-CN, and α-LA mRNA abundance was higher at early lactation, whereas compared with early lactation, only CAT-1 and α-LA mRNA abundance was higher at peak lactation. The CAT-2b, y(+)LAT1, and b(0,+)AT mRNA abundance did not differ when comparing either prepartum or peak lactation to early lactation. Compared with peak lactation, postweaning mRNA abundance of CAT-1, ATB(0,+), α-LA, and ß-CN decreased, y(+)LAT2, CAT-2b, and b(0,+)AT remained unchanged, and y(+)LAT1 increased. The mRNA abundance of y(+)LAT2 increased from early postpartum to early lactation, and remained unchanged for CAT-1, ATB(0,+), α-LA, and ß-CN. From prepartum to peak lactation, the mRNA abundance of CAT-1, y(+)LAT2, and ATB(0,+) was positively correlated with that of ß-CN and α-LA. In conclusion, the expression of genes encoding for y(+)LAT1, CAT-2b, and b(0,+)AT remained unchanged in porcine mammary glands over prepartum to peak lactation period, whereas expression of genes encoding for CAT-1, ATB(0,+), and y(+)LAT2 was upregulated and positively correlated to expression of genes encoding for the mammary synthesized milk proteins ß-CN and α-LA.


Subject(s)
Amino Acid Transport Systems/genetics , Caseins/genetics , Lactalbumin/genetics , Lactation , Mammary Glands, Animal/metabolism , Animals , Female , Peripartum Period , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Swine , Weaning
3.
Theriogenology ; 75(5): 897-903, 2011 Mar 15.
Article in English | MEDLINE | ID: mdl-21167582

ABSTRACT

Sperm plasma membrane lipids have an important role to play in determining membrane fluidity and sperm motility. The objective of the present study was to determine whether there are differences in the lipid and fatty acid (FA) composition of boar sperm and seminal plasma in the ejaculates of boars having different sperm motilities. Semen was collected from two groups of boars having normal (> 60%; n = 53) or low (< 60%; n = 53) motility sperm and the semen was evaluated for motility, morphology and vitality. The semen was then centrifuged to separate the sperm from the seminal plasma and both were kept at -20 °C until analyzed for lipid content and FA profile by gas chromatography. Total antioxidant status (TAS) of seminal plasma was determined using a commercial kit. There were differences (P ≤ 0.05) in sperm total lipids, cholesterol, saturated fatty acids (SFA), phospholipids, n-3 polyunsaturated fatty acids (PUFA), docosahexaenoic acid (DHA) and the ratio of n-6:n-3 PUFA between boars with normal and low motility sperm. Total lipids, cholesterol, phospholipids, PUFA, DHA and n-3 PUFA were positively correlated with sperm motility, viability, normal morphology and normal plasma membrane. In contrast, SFA and the ratio of n-6: n-3 PUFA were negatively correlated (P ≤ 0.05) with sperm motility, viability, normal morphology and normal plasma membranes. The TAS of seminal plasma from boars having normal motility sperm was higher (P ≤ 0.05) than that of boars having low motility sperm and TAS was positively correlated (P = 0.0001) with sperm motility, viability, normal morphology and normal plasma membranes. In summary, differences in sperm motility were related to n-3 PUFA content in the sperm plasma membrane and extracellular antioxidants in seminal plasma which protect sperm plasma membranes from lipid peroxidation during periods of oxidative stress.


Subject(s)
Lipids/analysis , Semen/chemistry , Sperm Motility/physiology , Spermatozoa/chemistry , Swine/physiology , Animals , Antioxidants/analysis , Cell Membrane/chemistry , Cholesterol/analysis , Docosahexaenoic Acids/analysis , Fatty Acids/analysis , Fatty Acids, Omega-3/analysis , Fatty Acids, Omega-6/analysis , Male , Phospholipids/analysis , Spermatozoa/abnormalities , Spermatozoa/ultrastructure
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