ABSTRACT
The novel allele HLA-B*08:62 differs from HLA-B* 08:01:01 by a nonsynonymous nucleotide substitution at position 24 of exon 2 (codon 9 changes from GAC to CAC).
Subject(s)
HLA-B Antigens/genetics , Alleles , Base Sequence , Exons , Family Health , Genetic Variation , HLA-B8 Antigen/genetics , Humans , Immunophenotyping/methods , Iraq , Molecular Sequence Data , Sequence Analysis, DNA/methodsABSTRACT
We report here the identification of a novel DRB1*11 allele, DRB1*1144, identified during sequence-based HLA-DRB1 typing. Molecular cloning and direct sequencing confirmed that the new allele is identical to DRB1*110401 at exon 2, except for a single nucleotide substitution (GTG-->GCG) changing codon 38 from Valine to Alanine.
Subject(s)
Alleles , HLA-DR Antigens/genetics , Amino Acid Substitution/genetics , Base Sequence , Cloning, Organism , HLA-DRB1 Chains , Humans , Molecular Sequence Data , Polymorphism, Single Nucleotide , Sequence AlignmentABSTRACT
This study was conducted in a rural community, São João dos Queiróz, a township in the county of Quixadá, Ceará, Brazil, using a combination of participatory research and community education in compliance with the health promotion reference and principles of the 1986 Ottawa Charter. The project was joined by representatives of several local government institutions and organizations from the grassroots community movement. The theme generating the research, as defined by an assembly meeting of the community association, was a diagnosis of sanitation conditions in the community. The starting point was the assessment of local conditions. Results showed adverse local conditions in sanitation, literacy, income, and employment. Suggestions for solving the problems were organized so as to be included in the planning agenda for local health policies. Evaluation was procedural and enriched with daily research activities. The problem-solving pedagogical approach developed during the educational process contributed to a critical reconstruction, appropriation, and sharing of the resulting knowledge.
Subject(s)
Health Education , Health Promotion , Sanitation , Adolescent , Adult , Brazil , Child , Child, Preschool , Community Participation , Educational Status , Female , Humans , Infant , Infant, Newborn , Male , Middle Aged , Research , Rural Population , Socioeconomic Factors , Water SupplyABSTRACT
A new MR technique is proposed to study the renal collecting system and the proximal ureteral portion in patients without obstructive uropathy, using fat-suppressed Turbo Spin Echo sequences, after drug-induced distension of the urinary tract. Nine normal volunteers and 16 patients (11 with renal stones, 1 with bilateral pyeloureteral junction stenosis, 1 with renal ptosis, 1 with ureteral hyperkinesia, 1 with renal tumor and 1 with a symptomatic renal cyst) were submitted to MR-Urography, performed with a 3D non-breath-hold fat-suppressed Turbo SE sequence (TR = 3000 ms, TE = 800 ms, 6 acquisitions, Turbo Factor = 128, Matrix = 128 x 256 or 256 x 256, acquisition time = 5 min 15 sec or 10 min 36 sec) on the coronal plane. With these acquisition parameters, parenchymal signal can be completely suppressed while enhancing fluid signal. These acquisitions were post-processed with the MIP algorithm to obtain very similar images to those of conventional urography. The maximum filling of the renal collecting system was obtained with the i.v. administration of 250 ml saline solution within 2-3 minutes and then the i.v. injection of 20 mg furosemide. The renal collecting system was optimally depicted in all the volunteers and the patients, except for 2 cases because of malfunctioning respiratory gating. Anatomical detailing was really improved after the diuretic administration, especially in the study of the major caliceal systems. To conclude, MR-Urography permits accurate morphological detailing of the renal collecting system also in patients without obstructive uropathy.
Subject(s)
Kidney Diseases/diagnostic imaging , Kidney Diseases/pathology , Kidney/anatomy & histology , Kidney/diagnostic imaging , Adult , Aged , Aged, 80 and over , Female , Humans , Magnetic Resonance Imaging , Male , Middle Aged , RadiographyABSTRACT
Peritoneal dialysis catheters are at risk for exit-site contamination when swimming or bathing. Also in pre-toilet trained children fecal and urine contamination can occur. The use of Tegaderm dressings prevents contamination and thus minimizes restriction of activity in children.
