Cocoa-associated filamentous fungi for the biocontrol of aflatoxigenic Aspergillus flavus.

Aflatoxin and other mycotoxin contamination are major threats to global food security and present an urgent need to secure the global food crop against spoilage by mycotoxigenic fungi. Cocoa material is noted for naturally low aflatoxin contamination. This study was designed to assess the potential for harnessing cocoa-associated filamentous fungi for the biocontrol of aflatoxigenic Aspergillus flavus. The candidate fungi were isolated from fermented cocoa beans collected from four cocoa-growing areas in Ghana. Molecular characterization included Internal Transcribed Spacer (ITS)-sequencing for identification and polymer chain reaction (PCR) to determine mating type. Effects of the candidate isolates on growth and aflatoxin-production by an aflatoxigenic A. flavus isolate (BANGA1) were assessed. Aflatoxin production was monitored by UV fluorescence and quantified by enzyme-linked immunosorbent assay (ELISA). Thirty-six filamentous fungi were cultured and identified as Aspergillus, Cladosporium, Lichtheimia, or Trichoderma spp. isolates. The isolates generally interacted negatively with BANGA1 growth and aflatoxin production. The Aspergillus niger and Aspergillus aculeatus biocontrol candidates showed the strongest colony antagonism (54%-94%) and reduction in aflatoxin production (12%-50%) on agar. In broth, the A. niger isolates reduced aflatoxin production by up to 97%. Metabolites from the A. niger isolates showed the strongest inhibition of growth by BANGA1 and inhibited aflatoxin production. Four of the candidate isolates belonged to the MAT1-1 mating type and 12 identified as MAT1-2. This may be indicative of the potential for genetic recombination events between fungi in the field, a finding which is particularly relevant to the risk posed by A. flavus biocontrol measures that rely on atoxigenic A. flavus strains.

Understanding the influence of free nitrous acid on microalgal-bacterial consortium in wastewater treatment: A critical review.

Microalgal-bacterial consortium (MBC) constitutes a sustainable and efficient alternative to the conventional activated sludge process for wastewater treatment (WWT). Recently, integrating the MBC process with nitritation (i.e., shortcut MBC) has been proposed to achieve added benefits of reduced carbon and aeration requirements. In the shortcut MBC system, nitrite or free nitrous acid (FNA) accumulation exerts antimicrobial influences that disrupt the stable process performance. In this review, the formation and interactions that influence the performance of the MBC were firstly summarized. Then the influence of FNA on microalgal and bacterial monocultures and related mechanisms together with the knowledge gaps of FNA influence on the shortcut MBC were highlighted. Other challenges and future perspectives that impact the scale-up of the shortcut MBC for WWT were illustrated. A potential roadmap is proposed on how to maximize the stable operation of the shortcut MBC system for sustainable WWT and high-value biomass production.

Insights into the multi-targeted effects of free nitrous acid on the microalgae Chlorella sorokiniana in wastewater.

Microalgal-bacterial consortium process (MBCP) proposed as an alternative to the activated sludge process contains free nitrous acid (FNA). FNA antimicrobial influences on nitrifiers have been demonstrated. However, its influence on microalgae is largely unknown, limiting the system stability of MBCP. This study revealed the multi-targeted responses of a model wastewater microalgae, Chlorella sorokiniana, to FNA exposure through physiological and transcriptomic analyses. Results showed a concentration-dependent FNA-influence as both microalgal growth and photosynthesis (Fv/Fm, rETR, Y(II), NPQ) inversely correlated with FNA doses. Increased ROS, MDA content (5.0-fold), SOD (2.7-fold), and LDH (12.0-fold) activities in the treatments revealed FNA-induced oxidative pressure. Moreover, RNA-sequencing results revealed significantly downregulated genes related to photosynthesis, respiration, nitrogen metabolism, and tricarboxylic acid cycle. Comparatively, peroxisome, chlorophyll, and carotenoid genes were upregulated. These findings elucidate the inhibitory mechanisms of FNA on microalgae and contribute towards the prospective practical application of the MBCP system for sustainable wastewater treatment.

A review of biopolymer (Poly-ß-hydroxybutyrate) synthesis in microbes cultivated on wastewater.

The large quantities of non-degradable single use plastics, production and disposal, in addition to increasing amounts of municipal and industrial wastewaters are among the major global issues known today. Biodegradable plastics from biopolymers such as Poly-ß-hydroxybutyrates (PHB) produced by microorganisms are potential substitutes for non-degradable petroleum-based plastics. This paper reviews the current status of wastewater-cultivated microbes utilized in PHB production, including the various types of wastewaters suitable for either pure or mixed culture PHB production. PHB-producing strains that have the potential for commercialization are also highlighted with proposed selection criteria for choosing the appropriate PHB microbe for optimization of processes. The biosynthetic pathways involved in producing microbial PHB are also discussed to highlight the advancements in genetic engineering techniques. Additionally, the paper outlines the factors influencing PHB production while exploring other metabolic pathways and metabolites simultaneously produced along with PHB in a bio-refinery context. Furthermore, the paper explores the effects of extraction methods on PHB yield and quality to ultimately facilitate the commercial production of biodegradable plastics. This review uniquely discusses the developments in research on microbial biopolymers, specifically PHB and also gives an overview of current commercial PHB companies making strides in cutting down plastic pollution and greenhouse gases.

Simultaneous improvements on nutrient and Mg recoveries of microalgal bioremediation for municipal wastewater and nickel laterite ore wastewater.

Effects of different mixing ratios between synthetic municipal wastewater (MW) and magnesium (Mg2+)-enriched nickel laterite ore wastewater (NLOWW) on growth of Chlorella sorokiniana (C. sorokiniana), photosynthetic activities, cellular biocomposition, nutrient and Mg2+ removal were investigated in photobioreactors. In the culture without NLOWW, wrinkled cells were observed with low biomass production. The culture mixed with 0.13% NLOWW obtained 1.89-fold higher biomass yield, 3.77-fold enhanced photosynthetic activity (Fv/Fm value), and improved nutrient removal (nitrogen by 102.2%, phosphorus by 39.3%). However, excessive Mg2+ at 100% NLOWW produced highest reactive oxygen species suppressing microalgal growth. The Mg2+ removal capacity increased with NLOWW loading. Moreover, microalgal assimilation primarily contributed to nutrient removal while absorption was the dominant Mg2+ removal pathway. Carbohydrate content in biomass increased with Mg2+ loading. Finally, the approach for MW/NLOWW treatment was demonstrated as economically feasible with revenue of $75.6 per kilogram biomass through a comprehensive economic model.