ABSTRACT
BACKGROUND: Powdery mildew disease of cucurbits is caused mainly by Podosphaera fusca, which is one of the most important limiting factors in cucurbit production worldwide. Previously we reported that Bacillus amyloliquefaciens biocontrol strain SD-32 produces C17 bacillomycin D and [Ile 2002]surfactin, and that these metabolites play important roles in SD-32's biocontrol over cucumber gray mold disease. Our further investigation demonstrated that the culture broth and its supernatant suppressed cucumber powdery mildew disease in greenhouse experiments. However, the active principle(s) remained unknown. RESULTS: The active compound was isolated from the culture supernatant after anti-powdery mildew disease activity-guided purification and identified as prumycin. Prumycin significantly suppressed the disease, whereas bacillomycin D and [Ile 2002]surfactin did not. Prumycin did not induce the expression of plant defense genes (PR1a and VSP1), suggesting that it does not act via plant defense response. Light microscopic observations of prumycin-treated cucumber cotyledon suggested that prumycin inhibits the conidial germination of P. fusca. CONCLUSION: This study demonstrates that prumycin is a major factor in SD-32's suppression of cucumber powdery mildew disease. Our findings shed light for the first time on prumycin's role in biocontrol by Bacillus against this disease. © 2017 Society of Chemical Industry.
Subject(s)
Antifungal Agents/pharmacology , Bacillus amyloliquefaciens/chemistry , Cucumis sativus/microbiology , Plant Diseases/prevention & control , Amino Sugars/chemistry , Amino Sugars/metabolism , Amino Sugars/pharmacology , Antifungal Agents/chemistry , Antifungal Agents/metabolism , Ascomycota/drug effects , Ascomycota/physiology , Bacillus amyloliquefaciens/metabolism , Plant Diseases/microbiologyABSTRACT
We previously reported that Bacillus amyloliquefaciens biocontrol strain SD-32 produces powerful antifungal lipopeptides, C17 bacillomycin D homologues. In the course of the investigation we found that the antifungal activity of the culture supernatant of this bacterium was not ascribed exclusively to bacillomycin D. We attempted to identify metabolites other than bacillomycin D to gain insight into the mechanism for the biocontrol by this bacterium. After purifying the fractions of the culture supernatant exhibiting synergistic activity with bacillomycin D, we isolated two new cyclic lipodepsipeptides, anteiso-C13 and iso-C13 [Ile(7)]surfactins, together with three known [Ile(7)]surfactins. Interestingly, [Ile(7)]surfactins showed synergistic activities with bacillomycin D to gray mold disease on cucumber leaves but not to Botrytis cinerea itself in vitro, suggesting that the synergistic effects might be on infection processes of the fungus. Actually, we observed that they did not show synergistic actions on conidial germination or mycelial growth of B. cinerea on the leaves.
