ABSTRACT
AIM: Lead acetate impairs testicular function by enhancing testicular oxidative stress and apoptosis. Cyperus esculentus possesses antioxidants and has shown great improvement of testicular function. This study investigated the protective effect of hydro-ethanolic extract of Cyperus esculentus on lead acetate-induced testicular dysfunction in Wistar rats. MATERIALS AND METHODS: Twenty-five male Wistar rats (180-195 g) were randomly divided into 5 groups (n = 5) namely: Normal control (NC), Lead control (PbC) (20 mg/kg b.w. i.p.), C. esculentus-treated (CE) (500 mg/kg b.w p.o.), Pb + CE(500) (20 mg/kg of lead and 500 mg/kg of extract) and Pb + CE(1000) (20 mg/kg of lead and 1000 mg/kg of extract). Administration lasted for 21 days. RESULTS: Sperm count, motility, viability, serum testosterone and follicle stimulating hormone, Johnsen's score, Leydig cell count, Sertoli cell count, testicular testosterone, B-cell lymphoma protein-2 (Bcl-2), steroidogenic acute regulatory protein, cytochrome P450 A1, 3ß-hydroxysteroid dehydrogenase (HSD), 17ß-HSD, enzymatic antioxidant activities and total antioxidant capacity were significantly (p < 0.05) decreased in PbC compared with NC. These parameters however increased significantly (p < 0.05) in Pb + CE(500) and Pb + CE(1000) compared with PbC. Lead acetate upregulated (p < 0.05) testicular malondialdehyde, nitric oxide, glucose, lactate, lactate dehydrogenase, C-reactive protein, tumor necrosis factor-α, interleukin (IL)-6, IL-1ß, Bcl-2 associated X (Bax), Bax/Bcl-2 and cleaved caspase-3 levels. All these parameters were downregulated (p < 0.05) in Pb + CE(500) and Pb + CE(1000) in comparison with PbC. CONCLUSION: C. esculentus exhibited a dose-dependent mitigation of lead acetate-induced testicular dysfunction in Wistar rats via its antioxidant, anti-inflammatory and anti-apoptotic effects.
Subject(s)
Anti-Inflammatory Agents/pharmacology , Antioxidants/pharmacology , Apoptosis/drug effects , Cyperus , Inflammation Mediators/metabolism , Oxidative Stress/drug effects , Plant Extracts/pharmacology , Testicular Diseases/prevention & control , Testis/drug effects , Animals , Anti-Inflammatory Agents/isolation & purification , Antioxidants/isolation & purification , Apoptosis Regulatory Proteins/metabolism , Cyperus/chemistry , Cytoprotection , Disease Models, Animal , Ethanol/chemistry , Follicle Stimulating Hormone/blood , Leydig Cells/drug effects , Leydig Cells/metabolism , Leydig Cells/pathology , Lipid Peroxidation/drug effects , Luteinizing Hormone/blood , Male , Organometallic Compounds , Plant Extracts/isolation & purification , Rats, Wistar , Signal Transduction , Solvents/chemistry , Spermatozoa/drug effects , Spermatozoa/metabolism , Spermatozoa/pathology , Testicular Diseases/chemically induced , Testicular Diseases/metabolism , Testicular Diseases/pathology , Testis/metabolism , Testis/pathology , Testosterone/bloodABSTRACT
High salt diet (HSD) impairs testicular function via oxidative stress. Cyperus esculentus contains antioxidants and improves testicular function. We investigated the protective effect of hydro-ethanolic extract of Cyperus esculentus on testicular function in HSD-fed Wistar rats. Twenty-five male Wistar rats (125-135 g) 8-9 weeks old were divided into five groups (n = 5): control, HSD-fed (8 % NaCl in feed), extract-treated (500 mg kg-1 day-1 ), HSD-fed +500 mg kg-1 day-1 of extract and HSD-fed +1,000 mg kg-1 day-1 of extract groups. Treatment lasted for 6 weeks. HSD decreased (p < .05) sperm parameters and serum reproductive hormones levels, while Cyperus esculentus extract improved (p < .05) sperm parameters, and serum testosterone and follicle-stimulating hormone levels in HSD-fed rats. The extract upregulated intra-testicular testosterone level and activities of 3ß-hydroxysteroid dehydrogenase (3ß-HSD) and 17ß-HSD, downregulated malondialdehyde and nitric oxide levels, and exhibited a dose-dependent decrease in pro-inflammatory cytokines, upregulation of activities of enzymatic antioxidants and increase in total antioxidant capacity in testes of HSD-fed rats. The extract at both doses improved Johnsen's score, Leydig and Sertoli cell counts and seminiferous tubular diameter in HSD-fed rats. Cyperus esculentus exhibited a dose-dependent mitigation of HSD-associated testicular dysfunction by targeting testicular steroidogenesis, oxidative stress and inflammation.
