ABSTRACT
To establish a novel method for the efficient isolation of Chlamydia pneumoniae, experiments were performed to determine the effects of EDTA or trypsin treatment of C. pneumoniae on its adsorption and inclusion body formation. Treatment of C. pneumoniae with 0.1% trypsin or 1 mM EDTA significantly increased inclusion body-forming activity from 8,000- to 10,000-fold higher than that of the control. C. pneumoniae was successfully isolated in cultured cells which were inoculated with clinical specimens after treatment with 0.1% trypsin.
Subject(s)
Bacteriological Techniques , Chlamydophila pneumoniae/isolation & purification , Edetic Acid , Trypsin , Cell Line , Chelating Agents/pharmacology , Chlamydophila pneumoniae/drug effects , Chlamydophila pneumoniae/growth & development , DNA, Bacterial/analysis , Ear, Middle/microbiology , Edetic Acid/pharmacology , Humans , Inclusion Bodies/drug effects , Lung , Pharynx/microbiology , Polymerase Chain Reaction/methods , Sputum/microbiology , Trypsin/pharmacologySubject(s)
Anti-Arrhythmia Agents/therapeutic use , Tachycardia, Ventricular/drug therapy , Adolescent , Adult , Aged , Anti-Arrhythmia Agents/administration & dosage , Electrophysiology , Female , Humans , Male , Middle Aged , Prognosis , Recurrence , Tachycardia, Ventricular/mortality , Tachycardia, Ventricular/physiopathologyABSTRACT
A 48-year-old female was admitted to our hospital for examination of an abnormal shadow in the right lung field. She had a systolic murmur (4/6) over the apex and the chest radiograph revealed cardiac enlargement with three round opacities in the right lung field. Cardiac catheterization showed marked mitral regurgitation and large pulmonary varices. Pulmonary varix caused by mitral regurgitation was diagnosed. The size of the pulmonary varix was reduced with improvement of pulmonary artery wedge pressure one month after mitral valve replacement. We conclude that pulmonary varices can decrease in size secondary to lowering of left atrial pressure within one month after operation.
