ABSTRACT
Incubation of isolated rat islets of Langerhans with [adenine-2,8-3H]NAD+ results in rapid incorporation of 3H into acid-insoluble products. The major site of incorporation appears to be the cell membrane. The reaction is inhibited by nicotinamide, an ADP-ribosylation inhibitor, and stimulated by arginine, an ADP-ribose acceptor. The results demonstrate that islet membrane proteins can be ADP-ribosylated in the absence of exogenous ADP-ribosylating agents and suggest that ADP-ribosylation plays a role in pancreatic islet cell function.
Subject(s)
Adenosine Diphosphate Ribose/metabolism , Islets of Langerhans/metabolism , Nucleoside Diphosphate Sugars/metabolism , Animals , Arginine/pharmacology , Cell Membrane/metabolism , In Vitro Techniques , Kinetics , NAD/metabolism , Niacinamide/pharmacology , Rats , Rats, Inbred Lew , Theophylline/pharmacology , TritiumABSTRACT
Fourteen cases of bronchogenic carcinoma were found in 191 patients with chronic lymphocytic leukemia seen at the Rosewell Park Memorial Institute, Buffalo, NY, from 1951 to 1976. Four of these were not diagnosed until the time of autopsy. There was an average lag of nine months between the onset of symptoms and signs suggestive of bronchogenic carcinoma and its diagnosis. In patients with chronic lymphocytic leukemia, a high index of suspicion for bronchogenic carcinoma is necessary fo its early detection.
Subject(s)
Carcinoma, Bronchogenic/complications , Carcinoma, Squamous Cell/complications , Leukemia, Lymphoid/complications , Lung Neoplasms/complications , Neoplasms, Multiple Primary , Adult , Aged , Antineoplastic Agents/therapeutic use , Carcinoma, Bronchogenic/drug therapy , Carcinoma, Bronchogenic/radiotherapy , Carcinoma, Squamous Cell/drug therapy , Carcinoma, Squamous Cell/radiotherapy , Drug Therapy, Combination , Humans , Leukemia, Lymphoid/drug therapy , Lung Neoplasms/drug therapy , Lung Neoplasms/radiotherapy , Male , Middle Aged , Neoplasms, Multiple Primary/drug therapy , Neoplasms, Multiple Primary/radiotherapyABSTRACT
Diabetes mellitus is satisfactorily controlled in the rat by hepatic implantation of isolated, isologous pancreatic islets. The transplanted islets appear to be viable for at least 6 months after implantation, and hepatic function studies (serum bilirubin, alkaline phosphatase, glutamic-oxalacetic transaminase, prothrombin time) and microscopic examination indicate that they do not interfere with hepatic function.
