LIF spectroscopy of epithelial tissues: Assay of structural changeover due to the cancer progression.

Most of the human cancers occur in epithelial tissues containing basic cells with different shapes, and not only do the spectral properties of the tissue pigments alter due to cancer, but the cellular architecture also change. However, in optical diagnosis of the cancerous tissues, attention has been paid to the spectral changeover of native chromophores as bio-markers. Here, we have attempted to assay the structural alterations of the epithelial tissues during the cancer progression utilizing Laser induced fluorescence (LIF) spectroscopy as a fast, sensitive and easy-to-use method. In this regard, angular dependence of the LIF spectral features of the healthy and cancerous epithelial tissues (soaked in Rhodamine 6G solution) from three different human organs i. e. uterus, colon and kidney with distinct microstructures have been examined. In general, both wavelength and intensity at the peak of the LIF spectra depend on the tissue orientation and the angle of detection respect to the laser beam direction. Those optical parameters also demonstrate distinctive alterations in different tissues that is explicated based on the morphological alteration of the epithelial cells in each carcinoma type provided by pathology data.

Optical characterization of the liver tissue affected by fibrolamellar hepatocellular carcinoma based on internal filters of laser-induced fluorescence.

Laser-induced fluorescence (LIF) spectroscopy has recently gained regards for diagnosis of the cancer in various tissues of the human body. This method in its conventional form, when used for assay of highly scattering media, encounters a lot of noise due to multiple scattering and inner filter effects which overshadows the sensitivity and specificity of the method. Here, angular dependence of the LIF spectral shift due to the reabsorption events have been investigated for characterization of the bio-tissues. The aim was to determine the tissue morphological changeovers due to the cancer progression. The assessment of a rare type of the liver cancer i. e. fibrolamellar hepatocellular carcinoma revealed the significant difference in optical anisotropy of the parenchyma and liver tumor. As a result, utilizing LIF spectroscopy as a fast, highly sensitive and easy-to-use method one can evaluate the optical anisotropy for diagnosing tissues during the cancer progression.