ABSTRACT
In Brazil, 4.5% of the AIDS-related opportunistic infections are caused by Cryptococcus neoformans. This pathogen is a ubiquitous environmental basidiomycetous encapsulated yeast, commonly found in soil and avian excreta. The present study investigates further the population structure of clinical and environmental C. neoformans isolates from south Brazil. One hundred five clinical and 19 environmental (pigeon excreta and Eucalyptus spp.) isolates from the Brazilian state Rio Grande do Sul were characterized based on morphological, biochemical, molecular and serological data. The majority of the clinical and environmental isolates analyzed belonged to C. neoformans var. grubii serotype A (89.5 and 52.6%, respectively), were mating type alpha (98.1 and 94.7%, respectively) and were phospholipase-positive (94.3 and 73.7%, respectively). PCR-fingerprinting with the microsatellite-specific primer M13 and the minisatellite-specific primer (GACA)(4) grouped the majority of the isolates into the molecular type VNI (89.5 of the clinical and 52.6% of the environmental isolates). Our results add considerable new information to the few available data on ecology, molecular biology and epidemiology of C. neoformans in the southern region of Brazil.
Subject(s)
Cryptococcus neoformans/classification , AIDS-Related Opportunistic Infections/epidemiology , AIDS-Related Opportunistic Infections/microbiology , Adult , Aged , Aged, 80 and over , Animals , Brazil/epidemiology , Columbidae/microbiology , Cryptococcosis/epidemiology , Cryptococcosis/microbiology , Cryptococcus neoformans/enzymology , Cryptococcus neoformans/genetics , DNA Fingerprinting , DNA, Fungal/chemistry , DNA, Fungal/genetics , Eucalyptus/microbiology , Feces/microbiology , Female , Humans , Male , Microsatellite Repeats/genetics , Middle Aged , Molecular Epidemiology , Phospholipases/metabolism , Polymerase Chain ReactionABSTRACT
Relatamos um caso de isolamento de Mycobacterium tuberculosis de cateter venoso central. O paciente apresentava tuberculose miliar e nenhum sinal de imunossupressao. O tramento com tuberculostaticos foi efetivo
Subject(s)
Humans , Adult , Bacteremia/microbiology , Catheterization, Central Venous/adverse effects , Tuberculosis/therapy , Antitubercular Agents/therapeutic use , Bacteremia/immunology , Mycobacterium tuberculosis/isolation & purification , Radiography, Thoracic , Tuberculosis/diagnosisABSTRACT
Descreve-se um surto (sete casos) de tinha da pele glabra ocasionado pelo Microsporum gypseum, caracterizando a fonte da infecçäo. Discute-se a raridade desta micose comparada a freqüência com que este dermatófito geofílico é encontrado no solo
