ABSTRACT
We aimed to assess the effects of melatonin in the in vitro production of bovine embryos. Our experiment was conducted at the Laboratório de Reprodução Animal of the Universidade Estadual do Maranhão. The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. The results from the first experiment demonstrated no significant difference between the in vitro maturation rate of the cultivated COCs in treatments with melatonin. In the second experiment, however, melatonin treatments yielded statistically higher cleavage, morula and blastocyst rates in the 10-5 µM group (52.9%, 52.9%, and 35.3%, respectively), and lower rates in the 10-1 µM group (19.5%, 19.5% and 7.8%, respectively), compared to the others. The control group (no melatonin) and the 10-3 µM group showed similar results. We concluded that supplementation of melatonin in the in vitro maturation medium resulted in no improvement in the oocyte maturation rate, but in the in vitro production of embryos at different concentrations, the 10-5 µM group displayed better results, but with no improvement in the variables (P < 0.05).(AU)
Objetivou-se avaliar os efeitos da melatonina na produção in vitro de embriões bovinos. O experimento foi conduzido no Laboratório de Reprodução Animal da Universidade Estadual do Maranhão. Os complexos cumulus-oócitos (CCOs) foram distribuídos entre os tratamentos 0, 10-1, 10-3 e 10-5 µmol/L de melatonina. A avaliação foi dividida em dois experimentos, onde o primeiro avaliou o efeito dessas diferentes concentrações de melatonina (tratamentos) sobre a taxa de maturação dos CCOs e o segundo, o efeito desses tratamentos com melatonina sobre a produção in vitro de embriões bovinos. Os resultados no primeiro experimento demonstraram não haver diferença significativa na taxa de maturação in vitro dos CCOs cultivados no tratamento com melatonina. No entanto, o tratamento com melatonina no segundo experimento, as taxas de clivagens, mórulas e blastocistos, o grupo 10-5 µM foi estatisticamente superior (52,9%, 52,9% e 35,3%, respectivamente) e o grupo 10-1 µM inferior (19,5%, 19,5% e 7,8%, respectivamente) aos outros grupos. O grupo controle (sem melatonina) e o grupo 10-3 µM obtiveram resultados semelhantes. Concluiu-se que a suplementação da melatonina no meio de maturação in vitro não evidenciou melhoras na taxa de maturação dos oócitos, porém na produção in vitro de embriões em diferentes concentrações, o grupo 10-5 µM apresentou melhores resultados mesmo não havendo melhorias nas variáveis (P<0,05).(AU)
Subject(s)
Animals , Cattle , Embryo, Mammalian , In Vitro Techniques/veterinary , Melatonin , Oocytes , Ovarian Follicle , Embryo Culture Techniques , In Vitro Oocyte Maturation Techniques/veterinaryABSTRACT
We aimed to assess the effects of melatonin in the in vitro production of bovine embryos. Our experiment was conducted at the Laboratório de Reprodução Animal of the Universidade Estadual do Maranhão. The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. The results from the first experiment demonstrated no significant difference between the in vitro maturation rate of the cultivated COCs in treatments with melatonin. In the second experiment, however, melatonin treatments yielded statistically higher cleavage, morula and blastocyst rates in the 10-5 µM group (52.9%, 52.9%, and 35.3%, respectively), and lower rates in the 10-1 µM group (19.5%, 19.5% and 7.8%, respectively), compared to the others. The control group (no melatonin) and the 10-3 µM group showed similar results. We concluded that supplementation of melatonin in the in vitro maturation medium resulted in no improvement in the oocyte maturation rate, but in the in vitro production of embryos at different concentrations, the 10-5 µM group displayed better results, but with no improvement in the variables (P < 0.05).
Objetivou-se avaliar os efeitos da melatonina na produção in vitro de embriões bovinos. O experimento foi conduzido no Laboratório de Reprodução Animal da Universidade Estadual do Maranhão. Os complexos cumulus-oócitos (CCOs) foram distribuídos entre os tratamentos 0, 10-1, 10-3 e 10-5 µmol/L de melatonina. A avaliação foi dividida em dois experimentos, onde o primeiro avaliou o efeito dessas diferentes concentrações de melatonina (tratamentos) sobre a taxa de maturação dos CCOs e o segundo, o efeito desses tratamentos com melatonina sobre a produção in vitro de embriões bovinos. Os resultados no primeiro experimento demonstraram não haver diferença significativa na taxa de maturação in vitro dos CCOs cultivados no tratamento com melatonina. No entanto, o tratamento com melatonina no segundo experimento, as taxas de clivagens, mórulas e blastocistos, o grupo 10-5 µM foi estatisticamente superior (52,9%, 52,9% e 35,3%, respectivamente) e o grupo 10-1 µM inferior (19,5%, 19,5% e 7,8%, respectivamente) aos outros grupos. O grupo controle (sem melatonina) e o grupo 10-3 µM obtiveram resultados semelhantes. Concluiu-se que a suplementação da melatonina no meio de maturação in vitro não evidenciou melhoras na taxa de maturação dos oócitos, porém na produção in vitro de embriões em diferentes concentrações, o grupo 10-5 µM apresentou melhores resultados mesmo não havendo melhorias nas variáveis (P<0,05).
Subject(s)
Animals , Cattle , Embryo, Mammalian , Ovarian Follicle , Melatonin , Oocytes , In Vitro Techniques/veterinary , Embryo Culture Techniques , In Vitro Oocyte Maturation Techniques/veterinaryABSTRACT
This study aimed to evaluate the effect of rbST on in vitro production of Girolanda female embryos.Ovum Pick-Up (OPU) was performed to obtain the cumulus-oocyte complexes (COCs) were subjected to thePIV. Pretreatment of rbST in cows Girolando did not influence the embryo development rate in vitro production.(AU)
Subject(s)
Animals , Cattle , Cattle/embryology , Embryo Culture Techniques/methods , Embryo Culture Techniques/veterinary , Growth Hormone/analysisABSTRACT
The objective was to monitor ovarian follicular development cows Gir, submitted to superovulationprotocol. Using 10 heifers, subject to three exam sessions gynecologic these being on day 0, day 4 and day 7,where on day 0 performed before beginning the superovulation protocol in order to select those that showed nochange in the reproductive tract and had corpus luteum active, while on day 4 and day 7 held in the days ofsuperstimulatory protocol, in order to quantify the follicles and evaluate follicular growth each female. With themeasurement of the follicles, it resulted three-averages 7,6 mm; 7,7mm and 10,0mm for the respective day, day0, day 4 and day 7. It was concluded that cows submitted to superovulation protocol presented ovarian folliclegrowth over the days evaluated by ultrasound and significant number of corpora lutea at the moment of embryocollection.(AU)
Subject(s)
Animals , Female , Cattle/anatomy & histology , Cattle/physiology , Superovulation/physiology , BiotechnologyABSTRACT
This study had the objective of evaluate the quality and the proportion of COCs collected by follicularaspiration Girolanda females. Eight cows and ten heifers were submitted in four OPU sessions with an intervalof 15 days. The heifer category provides a larger amount of recovered structures (GIII and AT / DG) comparedto cows, but cows had higher amount of GII.(AU)
Subject(s)
Animals , Female , Cattle , Cattle , Embryo Culture Techniques/veterinaryABSTRACT
This study aimed to evaluate the effect of recombinant bovine somatotropin on the plasma levels ofprogesterone, estradiol, growth hormone and IGF-1. 8 cows Girolando were divided into three groups: control,250 mg rbST and 500 mg rbST. Blood samples were collected 60 days prior to aspiration, in 12-day intervals,with the samples taken on the same days of rbST applications. For hormone measurements, ELISA commercialkits were used. The hormone concentrations were subjected to the normal pattern Cramer-von Mises and foraverage of comparison, the test Student-Newman-Keuls (SNK) was used, with 5% probability. It was notpossible to measure the plasma concentration of GH hormone. The rbST administration had no effect on plasmaconcentrations of progesterone, estradiol and IGF-1 among the groups treated with 250 mg and 500 mg and theuntreated group, there was also no significant variance between the days of application and OPUs.(AU)
Subject(s)
Animals , Female , Cattle , Growth Hormone/adverse effects , Growth Hormone/analysis , Progesterone , Estradiol/adverse effects , Insulin-Like Growth Factor I/chemistryABSTRACT
The objective was to monitor ovarian follicular development cows Gir, submitted to superovulationprotocol. Using 10 heifers, subject to three exam sessions gynecologic these being on day 0, day 4 and day 7,where on day 0 performed before beginning the superovulation protocol in order to select those that showed nochange in the reproductive tract and had corpus luteum active, while on day 4 and day 7 held in the days ofsuperstimulatory protocol, in order to quantify the follicles and evaluate follicular growth each female. With themeasurement of the follicles, it resulted three-averages 7,6 mm; 7,7mm and 10,0mm for the respective day, day0, day 4 and day 7. It was concluded that cows submitted to superovulation protocol presented ovarian folliclegrowth over the days evaluated by ultrasound and significant number of corpora lutea at the moment of embryocollection.
Subject(s)
Female , Animals , Biotechnology , Cattle/anatomy & histology , Cattle/physiology , Superovulation/physiologyABSTRACT
This study had the objective of evaluate the quality and the proportion of COCs collected by follicularaspiration Girolanda females. Eight cows and ten heifers were submitted in four OPU sessions with an intervalof 15 days. The heifer category provides a larger amount of recovered structures (GIII and AT / DG) comparedto cows, but cows had higher amount of GII.
Subject(s)
Female , Animals , Cattle , Cattle , Embryo Culture Techniques/veterinaryABSTRACT
This study aimed to evaluate the effect of recombinant bovine somatotropin on the plasma levels ofprogesterone, estradiol, growth hormone and IGF-1. 8 cows Girolando were divided into three groups: control,250 mg rbST and 500 mg rbST. Blood samples were collected 60 days prior to aspiration, in 12-day intervals,with the samples taken on the same days of rbST applications. For hormone measurements, ELISA commercialkits were used. The hormone concentrations were subjected to the normal pattern Cramer-von Mises and foraverage of comparison, the test Student-Newman-Keuls (SNK) was used, with 5% probability. It was notpossible to measure the plasma concentration of GH hormone. The rbST administration had no effect on plasmaconcentrations of progesterone, estradiol and IGF-1 among the groups treated with 250 mg and 500 mg and theuntreated group, there was also no significant variance between the days of application and OPUs.
Subject(s)
Female , Animals , Cattle , Estradiol/adverse effects , Insulin-Like Growth Factor I/chemistry , Growth Hormone/analysis , Growth Hormone/adverse effects , ProgesteroneABSTRACT
This study aimed to evaluate the effect of rbST on in vitro production of Girolanda female embryos.Ovum Pick-Up (OPU) was performed to obtain the cumulus-oocyte complexes (COCs) were subjected to thePIV. Pretreatment of rbST in cows Girolando did not influence the embryo development rate in vitro production.