ABSTRACT
Cemetery soils most likely contain degradative bacteria which possibly have beneficial potencies. However, the bacterial exploration in these potencies is still limitedly conducted in Indonesia. The raw sequence data of total bacteria in the cemetery soils through metagenomic analysis have been revealed. The data were obtained by collecting soil samples from six spots of two major Cemetery areas, which were Pracimaloyo (P) and Bonoloyo (B), in Surakarta City, Central Java, Indonesia. The six sample spots consisted of two samples from P area with respectively 20 cm and 140 cm depths and four samples of each two samples from B area with 20 and 40 cm depths. The total DNA was subsequently extracted from the collected soils using ZymoBIOMICS DNA Miniprep Kit. The total DNA then was amplified using a couple of 16S rRNA primers through Illumina HiSeq 2500 PE250 (Novogen, Korea) environment system. The raw sequence data has been submitted to the National Center for Biotechnology Information (NCBI) with project ID PRJNA997385. The archived sequence can be accessed in the NCBI website with the following URLs https://www.ncbi.nlm.nih.gov/sra/PRJNA997385. A brief analysis of the sequence data showed that the most common phyla in 20 cm-depths were Proteobacteria (29.5%), Actinobacteria (21.6%), and Firmicutes (19.2%), while Actinobacteria were the most found in 140 cm-depths with 34.2% followed by Proteobacteria (21.9%) and Firmicutes (16.6%). This data would be the first report of total bacterial sequence from cemetery soils in Indonesia.
ABSTRACT
AIMS: The study aimed to determine the inhibition activity of a combination of Noni fruit extract and Pandan leaves extract toward bacterial isolates that cause dandruff and to determine the potential of extract combination as an anti-dandruff shampoo. SUBJECTS AND METHODS: The test bacteria were isolated from dandruff. The inhibition test of the extract combination was done by paper disc and well diffusion method. The identification of bacterial isolates was done in Balai Laboratorium Kesehatan Yogyakarta. RESULTS: The study showed that the density of bacteria isolated from dandruff was 3.4 × 107 CFU/g. There were 28 pure bacteria isolates from purification process. The combination of Noni and Pandan extracts (1:1 v/v) could inhibit the growth of 92.9% bacterial isolates. There were seven species of bacteria from a total of 28 pure isolates, namely: Bacillus licheniformis, Bacillus megaterium, Bacillus alvei, Bacillus subtilis, Micrococcus sp, Stomatococcus sp, and Pseudomonas sp. CONCLUSIONS: Based on the research, it can be concluded that a combination of Noni fruit extract and Pandan leaves extract inhibited the growth of bacteria isolated from dandruff, so the combination of the extracts has potential as an anti-dandruff shampoo.
