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1.
Sci Rep ; 12(1): 22147, 2022 12 22.
Article in English | MEDLINE | ID: mdl-36550146

ABSTRACT

Fucoidans (FUCs) are highly sulfated polysaccharides demonstrating multiple actions in different systems. Oxaliplatin (OXA) is a platinum-containing chemotherapeutic agent with several side effects that restrict its usage. The current study aimed to determine the potential effect of FUC in male rats with splenic dysfunction induced by OXA. Eighty adult male rats aged (8-9 weeks) weighing (190-230 g) were divided into four groups: (Group I: the control group): Rats were administrated normal saline; (Group II: controls treated by FUC): Rats were treated with FUC; (Group III: Splenic dysfunction group): Rats were treated with 8 mg/kg OXA. (IV: Splenic dysfunction treated by FUC): Rats were treated by OXA as Group III, then fucoidan was given. At the end of the experiment, blood was collected to determine red blood cells and white blood cells. Splenic tissues were divided into one part for biochemical assays, oxidative stress markers as MDA and catalase, inflammatory markers (TNF-alpha, IL6), and apoptotic markers (caspase 3) and gene expression of Nrf2, Mapk1 gene expression, and endoplasmic stress parameters and the other part was used for immunohistochemical and histopathological analysis. Compared to the OXA-induced splenic dysfunction group, FUC significantly decreased high levels of MDA, TNF- alpha, IL6, caspase-3, Mapk1, endoplasmic stress induced by OXA, and increased the level of catalase and Nrf2. Fucoidan has corrected the histopathological and immunohistochemical changes compared to the OXA-induced splenic dysfunction group. In conclusion, our findings suggest that fucoidan has a significant role in the treatment of splenic dysfunction induced by OXA.


Subject(s)
Interleukin-6 , NF-E2-Related Factor 2 , Rats , Male , Animals , Oxaliplatin/adverse effects , Catalase/pharmacology , Interleukin-6/pharmacology , Prospective Studies , Polysaccharides/pharmacology , Polysaccharides/therapeutic use , Oxidative Stress
2.
Molecules ; 27(12)2022 Jun 07.
Article in English | MEDLINE | ID: mdl-35744783

ABSTRACT

Introduction. Vigabatrin (VGB) is an antiepileptic drug that acts to irreversibly inhibit the γ-aminobutyric acid (GABA) transaminase enzyme, elevating GABA levels. Broad studies have established that long-term treatment and/or high doses of VGB lead to variable visual defects. However, little attention has been paid to its other side effects, especially those demonstrating cerebellar involvement. Sodium glucose-linked co-transporter 2 (SGLT2) inhibitors are antidiabetic agents with protective effects far greater than expected based on their anti-hyperglycemic effect. Method. Our study herein was designed to investigate the possible ameliorative effect of empagliflozin, the SGLT2 inhibitors, in VGB-induced cerebellar toxicity. A total of 40 male Wistar rats were allocated equally into 4 groups: Group I: control group; Group II: VGB group; Group III empagliflozin treated VGB group; and Group IV: empagliflozin treated group. All groups were subjected to the detection of cerebellar messenger RNA gene expression of silent mating type information regulation 2 homolog 1 (SIRT1) and Nucleoporin p62 (P62). Mammalian target of rapamycin (mTOR), adenosine monophosphate-activated protein kinase (AMPK), and beclin1 levels were assessed by the ELISA technique while malondialdehyde (MDA) level and superoxide dismutase (SOD) activity were detected spectrophotometrically. Immuno-histochemical studies, focusing on glial fibrillary acidic protein (GFAP) and S100 were performed, and the optical color density and the mean area percentage of GFAP positive astrocytes and the number of S 100 positive cells were also counted. Results. Following empagliflozin treatment, we documented significant upregulation of both SIRT1 and P62 mRNA gene expression. Additionally, AMPK, Beclin1 levels, and SOD activity were significantly improved, while both mTOR and MDA levels were significantly reduced. Conclusions. We concluded for the first time that empagliflozin efficiently ameliorated the VGB-induced disrupted mTOR/AMPK/SIRT-1 signaling axis with subsequent improvement of the autophagy machinery and mitigation of the oxidative and inflammatory cellular environment, paving the way for an innovative therapeutic potential in managing VGB-induced neurotoxicity.


Subject(s)
AMP-Activated Protein Kinases , Vigabatrin , Animals , Anticonvulsants/pharmacology , Beclin-1 , Benzhydryl Compounds , Glucosides , Male , Mammals , Rats , Rats, Wistar , Signal Transduction , Sirtuin 1/genetics , Superoxide Dismutase , TOR Serine-Threonine Kinases , Vigabatrin/adverse effects , gamma-Aminobutyric Acid
3.
J Microsc Ultrastruct ; 8(2): 69-74, 2020.
Article in English | MEDLINE | ID: mdl-32766121

ABSTRACT

INTRODUCTION: Zingerone is a nontoxic important extract of dry ginger plant. It is reported that zingerone has an anticancer property, strong anti-inflammatory and antimicrobial properties. AIM OF THE WORK: is to evaluate the possible protective effects of zingerone on ethanol-induced lesions on the jejunum of adult male albino rats. MATERIALS AND METHODS: twenty four adult male albino rats were used, divided into 3 groups; A control group (I); consisted of 8 rats, ethanol group (II); contained 8 rats and each rat given 50% v/v alcohol at a dose of 4 g/kg.bw orally for 15 days. Ethanol zingerone group (III); consisted of 8 rats, each received zingerone at a dose of 50 mg/kg and alcohol at the same previous dose daily and orally for 15 days. At the appropriate time, the specimens were taken and prepared for light and electron microscope study. RESULTS: Histological examination of jejunum sections of ethanol group (II) showed massive jejunal villi ulcerations with shedding of their surface epithelium, loss of the villous architecture and loss of the microvilli covering some enterocytes. Examination of ethanol zingerone group (III) showed evidence of improvement in the form of nearly normal architecture of the jejunal villi with few areas of ulcerations on the top of some villi and increased cells with mitotic activity. CONCLUSION: Accordingly, we can conclude that zingerone administration can remarkably ameliorate ethanol-induced enterotoxiciy and jejunal ulcerations in rats by its anti-inflammatory properties and by suppressing oxidative stress.

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