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J Biol Chem ; 267(34): 24207-16, 1992 Dec 05.
Article in English | MEDLINE | ID: mdl-1447170

ABSTRACT

The present study was designed to investigate the effects of aging on preferential sites of glucose adduct formation on type I collagen chains. Two CNBr peptides, one from each type of chain in the type I tropocollagen molecule, were investigated in detail: alpha 1(I)CB3 and alpha 2CB3-5. Together these peptides comprise approximately 25% of the total tropocollagen molecule. The CNBr peptides were purified from rat tail tendon, obtained from animals aged 6, 18, and 36 months, by ion exchange chromatography, gel filtration, and high-performance liquid chromatography (HPLC). Sugar adducts were radiolabeled by reduction with NaB3H4. Glycated tryptic peptides were prepared from tryptic digests of alpha 2CB3-5 and alpha 1(I)CB3 by boronate affinity chromatography and HPLC. Peptides were identified by sequencing and by compositional analysis. Preferential sites of glycation were observed in both CB3 and alpha 2CB3-5. Of the 5 lysine residues in CB3, Lys-434 was the favored glycation site. Of the 18 lysine residues and 1 hydroxylysine residue in alpha 2CB3-5, 3 residues (Lys-453, Lys-479, and Lys-924) contained more than 80% of the glucose adducts on the peptide. Preferential glycation sites were highly conserved with aging. In collagen that had been glycated in vitro, the relative distribution of glucose adducts in old animals differed from that of young animals. In vitro experiments suggest that primary structure is the major determinant of preferential glycation sites but that higher order structure may influence the relative distribution of glucose adducts among these preferred sites.


Subject(s)
Aging/metabolism , Collagen/metabolism , Tendons/metabolism , Amino Acid Sequence , Amino Acids/analysis , Analysis of Variance , Animals , Chromatography, High Pressure Liquid , Collagen/chemistry , Cyanogen Bromide , Electrophoresis, Polyacrylamide Gel , Glucose/analysis , Glycosylation , Hydroxyproline/analysis , Male , Peptide Fragments/isolation & purification , Rats , Rats, Sprague-Dawley , Tendons/growth & development , Trypsin
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