New earthworm records from the Caspian Hyrcanian Forests of Iran (Oligochaeta: Megadrili).

Earthworms are the most important soil invertebrates worldwide, in terms of biomass and effects on soil processes. In this study, 21 earthworm species, including four new records were identified from Caspian Hyrcanian Forests (North of Iran). Four species; Criodrilus lacuum, Lumbricus rubellus, Metaphire californica, and Octodrilus transpadanus and the family Criodrilidae are reported for the first time. Previous studies have identified 31 earthworm species belonging to 14 genera and three families (Lumbricidae, Acanthodrilidae, and Megascolecidae) in Iran; therefore, these new records increase the number of earthworm species to a total of 35.

Integrative taxonomy of some Iranian peregrine earthworm species using morphology and barcoding (Annelida: Megadrili).

Despite the biological and economic importance of earthworms, the taxonomic status and evolutionary relationships of most lumbricid genera are still under debate. Further complications arise from the recognition that earthworms also show a high cryptic diversity. Past and current field studies of Iranian earthworm fauna have resulted in the identification of a total number of 28 earthworm species. However, many specimens do not fully fit into their original descriptions, making the species assignation very difficult. In this study, we evaluated the genetic diversity using mitochondrial markers as a tool to assess the species occurrence of some problematic species in Iran. Four species with high morphological variation were selected: Aporrectodea caliginosa (Savigny, 1826), Aporrectodea trapezoides (Dugès, 1828), Dendrobaena byblica (Rosa, 1893) and Dendrobaena veneta (Rosa, 1886). Morphological identification was contrasted with the molecular information generated through COI and 16S barcoding and the COI and 16S sequences stored in the Genbank. The results of this first integrative taxonomic analysis revealed that D. veneta consisted of two separated clades and that a number of species assigned to D. byblica showed very close relationships with those belonging to the genus Philomontanus. The lack of taxonomic expertise and identification characters providing a clear and unambiguous identification of earthworms highlights the urgent need of new tools to identify species unequivocally. Therefore, it is concluded that more taxonomical studies are needed to clarify the diagnostic characters and taxonomic status of the species belonging to two genera, Aporrectodea and Dendrobaena (Lumbricidae), in Iran.

Comparative studies on reproductive traits of natural populations of Eisenia andrei Bouché, 1972 from Zagros Mountain, Iran (Annelida: Oligochaeta).

Comparative studies were carried out on growth and reproduction patterns of eight populations of Eisenia andrei (Bouché, 1972) from different parts of western Iran along the Zagros Mountains, considered to lie within the natural range of this species. Parameters were gain in body weight, number of cocoon production, hatching success, number of hatchlings, hatchlings growth and survival. There were significant differences in mean numbers of viable cocoons, incubation time, reproductive rate, growth rate and final weight between different populations. The resulting groupings did only partially coincide with genetic clusters established in a previous study. It is nonetheless suggested to include life cycle parameters as an additional set of data to understand the micro-taxonomic structure of variable species.

Two new species of Tetragonocephalum (Cestoda: Lecanicephalidea) from Pastinachus sephen (Myliobatiformes: Dasyatidae) from the Gulf of Oman.

In the present study two new species of Tetragonocephalum Shipley et Hornell, 1905, T. mackenziei sp. n. and T. kazemii sp. n., are described from the spiral intestine of the cowtail stingray, Pastinachus sephen (Forsskål), from the northern coast of the Gulf of Oman. Tetragonocephalum mackenziei is distinguished from the 16 other valid species of Tetragonocephalum by a unique combination of characteristics, i.e. sperm-filled seminal receptacle in immature proglottids, body length (7.7-17.5 mm), body width (213-288 µm), number of proglottids (34-49), number of testes (10-14), size of scolex (228-315 µm × 213-288 µm) and size of acetabula (56-73 µm × 61-75 µm). Tetragonocephalum kazemii is morphologically distinguishable from its valid congeners and T. mackenziei based on a combination of characteristics, including body length (28.8-36.6 mm), number of proglottids (50-65), number of testes (30-42), size of scolex (388-564 µm × 326-448 µm), size of acetabula (62-86 µm × 57-90 µm) and testes (25-39 × 21-32). This brings the total number of validly described species of Tetragonocephalum to 18 and expands our knowledge of this diverse genus to now include the Gulf of Oman, as well as Arafura Sea, northern Indian Ocean and western Pacific Ocean.

Two new cestode species of Tetragonocephalum Shipley & Hornell, 1905 (Lecanicephalidea, Tetragonocephalidae) from Himantura randalli Last, Manjaji-Matsumoto & Moore (Myliobatiformes, Dasyatidae) from the Gulf of Oman.

The original description of the genus Tetragonocephalum was published more than one hundred years ago but its taxonomic status was clarified only recently. To date, approximately 30 nominal species of this genus have been described, mostly from the northern Indian Ocean, but nearly half of them are invalid and only 14 species are recognized as valid. In the present study two new species of Tetragonocephalum are described from the spiral intestine of Himantura randalli from off Jod, on the northern coast of the Gulf of Oman. Tetragonocephalum sabaesp. n. is distinguishable from the valid species of Tetragonocephalum based on number of proglottids (43-53), number of testes (42-50), and size of scolex (401-453×328-455), acetabula (87-109×72-116), mature proglottids (802-1,333×226-336), cirrus sac (92-160×103-154), and eggs (16-19×11-13). Tetragonocephalum salariisp. n. can be distinguished from Tetragonocephalum sabaesp. n. and all other valid species of Tetragonocephalum based on number of proglottids (77-86). Furthermore, it differs from its congeners based on a combination of some characteristics, including the number of mature (3-7) and gravid (18-20) proglottids, the number of testes (30-38), and the size of acetabula (84-111×80-96), mature proglottids (497-833×334-403), gravid proglottids (1,036-1,482×440-575), testes (20-34×31-50), ovary (123-215×210-278), and eggs (24-45×13-21).