ABSTRACT
Microbial analysis has become one of the most critical areas in aquatic ecology and a crucial component for assessing the contribution of microbes in food web dynamics and biogeochemical processes. Initial research was focused on estimating the abundance and distribution of the microbes using microscopy and culture-based analysis, which are undoubtedly complex tasks. Over the past few decades, microbiologists have endeavored to apply and extend molecular techniques to address pertinent questions related to the function and metabolism of microbes in aquatic ecology. Metagenomics analysis has revolutionized aquatic ecology studies involving the investigation of the genome of a mixed community of organisms in an ecosystem to identify microorganisms, their functionality, and the discovery of novel proteins. This review discusses the metagenomics analysis of bacterial diversity in and around different aquatic systems in India.
Subject(s)
Ecosystem , Metagenome , Bacteria/genetics , Bacteria/metabolism , Metagenomics/methods , IndiaABSTRACT
Infectious diseases caused by bacterial species of the Vibrio genus have had considerable significance upon human health for centuries. V. cholerae is the causative microbial agent of cholera, a severe ailment characterized by profuse watery diarrhea, a condition associated with epidemics, and seven great historical pandemics. V. parahaemolyticus causes wound infection and watery diarrhea, while V. vulnificus can cause wound infections and septicemia. Species of the Vibrio genus with resistance to multiple antimicrobials have been a significant health concern for several decades. Mechanisms of antimicrobial resistance machinery in Vibrio spp. include biofilm formation, drug inactivation, target protection, antimicrobial permeability reduction, and active antimicrobial efflux. Integral membrane-bound active antimicrobial efflux pump systems include primary and secondary transporters, members of which belong to closely related protein superfamilies. The RND (resistance-nodulation-division) pumps, the MFS (major facilitator superfamily) transporters, and the ABC superfamily of efflux pumps constitute significant drug transporters for investigation. In this review, we explore these antimicrobial transport systems in the context of Vibrio spp. pathogenesis and virulence.
ABSTRACT
Mud banks and upwelling are two important oceanographic features occurring along the southwest coast of India during the southwest monsoon period. The study region, Alappuzha lying on the southwest coast of India, is unique due to the co-existence of upwelling and mud banks during the monsoon (MON) season. Water samples were collected from three stations, M1, M2, and M3, from April to September 2014, at weekly/biweekly intervals to determine the total bacterial abundance, viable prokaryotic counts, and total plate counts, along with measurements on physico-chemical parameters. For determining the heterotrophic culturable bacterial diversity, water samples were collected during two seasons, monsoon and pre-monsoon (PRM), from three stations. Water samples were inoculated into two non-selective broths for enrichment, DNA was extracted, and next-generation sequencing analysis was performed using Illumina Miseq sequencing. The sequence analysis revealed that dominant communities were Proteobacteria, followed by Firmicutes and Fusobacteria. Proportions of Fusobacteria increased during monsoon and proportions of Firmicutes were high in premonsoon season. Among Proteobacteria, Gammaproteobacteri is presented more than 99% of all the classes, irrespective of seasons. Vibrio was the most dominant genus during both seasons. The presence of anaerobic genera such as Propionigenium and Cetobacterium at all the stations during MON indicated the presence of upwelled waters. The genus Stenotrophomonas was observed in the M2 station alone. This study provides an overview of the culturable heterotrophic bacterial communities in a region in the southeastern Arabian Sea with coexisting mud banks and upwelling. The results of this study were compared with a published report on culture-independent bacterial diversity (from environmental DNA) from the same region. The study demonstrates that the use of culture media underrepresented the phylogenetic diversity and selectively enriched the class Gammaproteobacteria alone.
Subject(s)
Bacteria , Proteobacteria , Bacteria/genetics , Heterotrophic Processes , Phylogeny , Seasons , SeawaterABSTRACT
Anthropogenic contamination of coastal-marine water is responsible for introducing multidrug-resistant bacteria such as the pNDM-harbouring Escherichia coli into the seafood chain. This study was conducted to understand the survivability of a multidrug-resistant, the New Delhi Metallo-ß-lactamase-producing E. coli (AS-EC121) in tropical seawater at room temperature (28-32 °C) compared to E. coli K12 strain. The experimental and control strains were inoculated at 6 log CFU/ml level into seawater. After an initial sharp decline in counts, AS-EC121 and K12 strains showed a gradual loss of viability after week-1 of inoculation. AS-EC121 was undetectable after day-56, while K12 colonies disappeared a week later, from day-63. The conjugation experiment revealed that pNDM was transferable to a recipient E. coli strain in seawater. This study suggests that the multidrug-resistant, pNDM-harbouring E. coli is able to survive in seawater for over 2 months stably maintaining the resistance plasmid. The resistance genotypes do not seem to compromise the survivability of MDR E. coli and the stability of plasmid provides ample opportunities for dissemination of plasmids among co-inhabiting bacteria in the coastal-marine environments.
Subject(s)
Conjugation, Genetic , Escherichia coli Proteins , Escherichia coli , Microbial Viability , Seawater , beta-Lactamases , Drug Resistance, Multiple, Bacterial/genetics , Escherichia coli/genetics , Escherichia coli Proteins/genetics , Microbial Sensitivity Tests , Microbial Viability/genetics , Plasmids/genetics , Seawater/microbiology , Tropical Climate , beta-Lactamases/geneticsABSTRACT
BACKGROUND: Seafood contamination with Salmonella enterica is not only a public health concern, but can also lead to economic losses due to import rejections. Continuous monitoring of seafood for Salmonella is necessary for risk assessment and to establish suitable control measures. OBJECTIVE: The objective of this study was to determine the extent of Salmonella contamination of seafood in fish landing centers and retails markets. METHODS: In this study, we analyzed seafood samples from three fish landing centers and seven retail markets of Mumbai, India for S. enterica contamination. Salmonella was isolated using multiple selective enrichment broths and selective agars, and identified by conventional biochemical tests followed by polymerase chain reaction (PCR). RESULTS: Of 82 samples of seafood, comprising both finfish and shellfish, Salmonella was detected in 17 (20.7%) finfish samples. None of the shellfish samples (shrimps, clams, and cephalopods) were found to be contaminated with Salmonella. Samples from both landing centers and fish markets, as well as from pelagic and demersal sources, harbored Salmonella. Source-wise analysis showed that 11 of 57 (19.3%) samples from retail fish markets and 6 of 25 (24%) samples from fish landing centers were contaminated with Salmonella. CONCLUSIONS: The study emphasizes the need to implement measures to prevent anthropogenic contamination of coastal waters and improve the hygiene of retail fish markets in Mumbai. HIGHLIGHTS: Seafood from landing centers and retail markets, as well as from pelagic and demersalhabitats were similarly contaminated with Salmonella. Fish species widely consumed in the region of this study were found highly contaminated with Salmonella.
Subject(s)
Salmonella , Seafood , Animals , Fishes , Food Contamination/analysis , India , ShellfishABSTRACT
The novel technology of biological carbon sequestration using microbial enzymes have numerous advantages over conventional sequestration strategies. In the present study, extracellular carbonic anhydrase (CA) producing bacteria were isolated from water samples in the Arabian Sea, India. A potential isolate, Bacillus safensis isolate AS-75 was identified based on 16S rDNA sequence analysis. The culture conditions suitable for CA production were 32 °C incubation temperature with 4% NaCl and 10 mM Zn supplementation. Experimental optimization of culture conditions enhanced enzyme activity to 265 U mL-1. CA specific gene was characterized and based on the analysis, the CA of B. safensis isolate AS-75 was a leucine (11.3%) with α-helices as the dominant component in its secondary structure. Based on soil microcosm studies, CA could sequester CO2 by 95.4% ± 0.11% in sterilized soil with enzyme microcosm. Hence, the application of enzyme was found to be more effective in removing CO2.
Subject(s)
Bacillus/enzymology , Carbon Dioxide/metabolism , Carbonic Anhydrases/metabolism , Seawater/microbiology , Amino Acid Sequence , Bacillus/chemistry , Bacillus/metabolism , Carbonic Anhydrases/chemistry , Carbonic Anhydrases/isolation & purification , Enzymes, Immobilized/chemistry , Enzymes, Immobilized/metabolism , Kinetics , Models, Molecular , Soil Microbiology , TemperatureABSTRACT
Biosynthesis of nanoparticles through plant extracts is gaining attention due to the toxic free synthesis process. The environmental engineering applications of many metal oxide nanoparticles have been reported. In this study, iron oxide nanoparticles (Fe2O3-Nps) were synthesised using a simple biosynthetic method using a leaf extract of a mangrove plant Rhizophora mucronata through reduction of 0.01â M ferric chloride. Fe2O3-Np synthesis was revealed by a greenish colour formation with a surface plasmon band observed close to 368â nm. The stable Fe2O3-Np possessed excitation and emission wavelength of 368.0 and 370.5â nm, respectively. The Fourier-transform infrared spectral analysis revealed the changes in functional groups during formation of Fe2O3-Np. Agglomerations of nanoparticles were observed during scanning electron microscopic analysis and energy-dispersive X-ray spectroscopic analysis confirmed the ferric oxide nature. The average particle size of Fe2O3-Np based on dynamic light scattering was 65â nm. Based on transmission electron microscopic analysis, particles were spherical in shape and the crystalline size was confirmed by selected area electron diffraction pattern analysis. The synthesised Fe2O3-Np exhibited a good photodegradation efficiency with a reduction of 83 and 95% of phenol red and crystal violet under irradiation of sunlight and florescent light, respectively. This report is a facile synthesis method for Fe2O3-Np with high photodegradation efficiency.
Subject(s)
Coloring Agents/metabolism , Magnetite Nanoparticles/chemistry , Plant Extracts/metabolism , Rhizophoraceae/metabolism , Coloring Agents/chemistry , Coloring Agents/radiation effects , Photolysis , Plant Leaves/chemistry , Rhizophoraceae/chemistryABSTRACT
Variants of the microorganism Staphylococcus aureus which are resistant to antimicrobial agents exist as causative agents of serious infectious disease and constitute a considerable public health concern. One of the main antimicrobial resistance mechanisms harbored by S. aureus pathogens is exemplified by integral membrane transport systems that actively remove antimicrobial agents from bacteria where the cytoplasmic drug targets reside, thus allowing the bacteria to survive and grow. An important class of solute transporter proteins, called the major facilitator superfamily, includes related and homologous passive and secondary active transport systems, many of which are antimicrobial efflux pumps. Transporters of the major facilitator superfamily, which confer antimicrobial efflux and bacterial resistance in S. aureus, are good targets for development of resistance-modifying agents, such as efflux pump inhibition. Such modulatory action upon these antimicrobial efflux systems of the major facilitator superfamily in S. aureus may circumvent resistance and restore the clinical efficacy of therapy towards S. aureus infection.
ABSTRACT
Food-borne pathogens are a serious human health concern worldwide, and the emergence of antibiotic-resistant food pathogens has further confounded this problem. Once-highly-efficacious antibiotics are gradually becoming ineffective against many important pathogens, resulting in severe treatment crises. Among several reasons for the development and spread of antimicrobial resistance, their overuse in animal food production systems for purposes other than treatment of infections is prominent. Many pathogens of animals are zoonotic, and therefore any development of resistance in pathogens associated with food animals can spread to humans through the food chain. Human infections by antibiotic-resistant pathogens such as Campylobacter spp., Salmonella spp., Escherichia coli and Staphylococcus aureus are increasing. Considering the human health risk due to emerging antibiotic resistance in food animal-associated bacteria, many countries have banned the use of antibiotic growth promoters and the application in animals of antibiotics critically important in human medicine. Concerted global efforts are necessary to minimize the use of antimicrobials in food animals in order to control the development of antibiotic resistance in these systems and their spread to humans via food and water.