Eficacia de la intervención psicológica en rehabilitación cardíaca / Effectiveness of psychological intervention in cardiac rehabilitation

OBJETIVO: El objetivo del presente estudio fue describir y analizar los resultados de la intervención cognitivo-conductual en los pacientes beneficiarios del programa multidisciplinar de rehabilitación cardíaca del Hospital General Universitario de Elche. MATERIAL Y MÉTODO: Para ello se dispuso de una muestra de 33 pacientes que habían tenido un infarto agudo de miocardio o que padecían angina de pecho inestable remitidos desde el Servicio de Cardiología para su inclusión en el Programa de Prevención y Rehabilitación Cardíaca durante los meses comprendidos en el período de septiembre de 2014 a octubre de 2015. Todos los participantes fueron evaluados mediante una batería de cuestionarios pre y postratamiento. RESULTADOS: Los resultados obtenidos mostraron una disminución estadísticamente significativa tras la intervención en gran parte de los factores de riesgo, como ansiedad (p < 0,001, d = 0,38), depresión (p < 0,005, d = 0,25) e ira (p < 0,005, d = 0,35). CONCLUSIONES: Este estudio constituye una nueva evidencia de la eficacia de la intervención psicológica en rehabilitación cardíaca

OBJECTIVE: The aim of this study was to describe and analyze the results of the cognitive-behavioral intervention in the patients beneficiaries of the multidisciplinary Cardiac Rehabilitation program of the Hospital General Universitario de Elche (Spain). MATERIAL AND METHOD: A sample that included 33 patients who had suffered an acute myocardial infarction or who suffer from unstable angina pectoris. These patients were referred from the Cardiology Department for inclusion in the Cardiac Prevention and Rehabilitation Program during the period from September 2014 to October 2015. All the patients were evaluated using a battery of pre- and post- treatment questionnaires. RESULTS: The results showed a statistically significant decrease in a large part of the risk factors after the intervention, such as anxiety (P < .001, d = 0.38), depression (P < .005, d = 0.25) and anger (P < .005, d = 0.35). CONCLUSIONS: This study provides new evidence of the effectiveness of psychological intervention in cardiac rehabilitation

[Effectiveness of psychological intervention in cardiac rehabilitation]. / Eficacia de la intervención psicológica en rehabilitación cardíaca.

OBJECTIVE: The aim of this study was to describe and analyze the results of the cognitive-behavioral intervention in the patients beneficiaries of the multidisciplinary Cardiac Rehabilitation program of the Hospital General Universitario de Elche (Spain). MATERIAL AND METHOD: A sample that included 33 patients who had suffered an acute myocardial infarction or who suffer from unstable angina pectoris. These patients were referred from the Cardiology Department for inclusion in the Cardiac Prevention and Rehabilitation Program during the period from September 2014 to October 2015. All the patients were evaluated using a battery of pre- and post- treatment questionnaires. RESULTS: The results showed a statistically significant decrease in a large part of the risk factors after the intervention, such as anxiety (P < .001, d = 0.38), depression (P < .005, d = 0.25) and anger (P < .005, d = 0.35). CONCLUSIONS: This study provides new evidence of the effectiveness of psychological intervention in cardiac rehabilitation.

Bayesian hierarchical Poisson models with a hidden Markov structure for the detection of influenza epidemic outbreaks.

Considerable effort has been devoted to the development of statistical algorithms for the automated monitoring of influenza surveillance data. In this article, we introduce a framework of models for the early detection of the onset of an influenza epidemic which is applicable to different kinds of surveillance data. In particular, the process of the observed cases is modelled via a Bayesian Hierarchical Poisson model in which the intensity parameter is a function of the incidence rate. The key point is to consider this incidence rate as a normal distribution in which both parameters (mean and variance) are modelled differently, depending on whether the system is in an epidemic or non-epidemic phase. To do so, we propose a hidden Markov model in which the transition between both phases is modelled as a function of the epidemic state of the previous week. Different options for modelling the rates are described, including the option of modelling the mean at each phase as autoregressive processes of order 0, 1 or 2. Bayesian inference is carried out to provide the probability of being in an epidemic state at any given moment. The methodology is applied to various influenza data sets. The results indicate that our methods outperform previous approaches in terms of sensitivity, specificity and timeliness.

Geographical spread of influenza incidence in Spain during the 2009 A(H1N1) pandemic wave and the two succeeding influenza seasons.

The aim of this study was to monitor the spatio-temporal spread of influenza incidence in Spain during the 2009 pandemic and the following two influenza seasons 2010-2011 and 2011-2012 using a Bayesian Poisson mixed regression model; and implement this model of geographical analysis in the Spanish Influenza Surveillance System to obtain maps of influenza incidence for every week. In the pandemic wave the maps showed influenza activity spreading from west to east. The 2010-2011 influenza epidemic wave plotted a north-west/south-east pattern of spread. During the 2011-2012 season the spread of influenza was geographically heterogeneous. The most important source of variability in the model is the temporal term. The model of spatio-temporal spread of influenza incidence is a supplementary tool of influenza surveillance in Spain.

Flow cytometric analysis of a marine LAS-degrading consortia.

The specific nucleic acid fluorochrome SYTO-13 was used in flow cytometric analysis to assess changes in the density and heterogeneity of marine bacterial populations which biodegrade linear alkylbenzene sulphonate (LAS). Seawater samples with LAS and incubated in the laboratory (20 degrees C, 100 rpm, 30 days) were used to monitor LAS-degrading consortia. Flow cytometric studies and culture methods were used to characterize the LAS degrading bacterioplankton consortia. Fluorescence and scatter signals enabled us to define three regions (R1, R2 and R3) in the dual parameter cytograms. The distribution of the bacterial counts in these regions allowed us to monitor the formation and evolution of the consortia.

Exogenous isolation of Hgr plasmids from coastal Mediterranean waters and their effect on growth and survival of Escherichia coli in sea water.

Four self-conjugative plasmids conferring mercury resistance were exogenously isolated from coastal Mediterranean sea water. All the plasmids isolated (93.2, 73.1, 65.2 and 221 kb) showed narrow-spectrum mercury resistance, and no UV resistance; three of them conferred cadmium resistance (Cdr 1.5 mM) and two of them encoded streptomycin resistance (50 micrograms/ml). Their influence on the survival of the recipient strain Escherichia coli JGL1 in different seawater media was studied and compared with that of two plasmids previously isolated by the same procedure from an epilithic community of the Bristol Channel, Great Britain. A significant negative influence was observed for the largest (221 kb) of the plasmids isolated and one of the epilithic plasmids (239 kb). The effect of the plasmids on E.coli JGL1 was dependent on the seawater media.

Assessment of E. coli and Salmonella viability and starvation by confocal laser microscopy and flow cytometry using rhodamine 123, DiBAC4(3), propidium iodide, and CTC.

Assessment of cell viability using methods which do not require cell culture is essential in the field of aquatic microbiology, since many bacteria known to be present in aquatic environments cannot be grown in culture. The study of bacterial biofilms, which previously needed an epifluorescent microscope, has recently been enhanced by the use of flow cytometry and confocal scanning laser microscopy (CSLM). A method based on the combination of several membrane potential related dyes, a membrane integrity dye and a redox probe was used to measure cell viability by flow cytometry and confocal laser microscopy. Rhodamine-propidium iodide (PI) double staining was used to discriminate viable from nonviable cells in CSLM observations. Membrane depolarization during E. coli and Salmonella starvation measured by DiBAC4(3) incorporation (flow cytometry and CSLM) was found to be in concordance with respiratory activity as detected by a tetrazolium salt (CTC) reduction.

Flow cytometric assessment of Escherichia coli and Salmonella typhimurium starvation-survival in seawater using rhodamine 123, propidium iodide, and oxonol.

The use of flow cytometry in microbiology allows rapid characterization of cells from a nonhomogeneous population. A method based on flow cytometry to assess the effects of lethal agents and the bacterial survival in starved cultures through the use of membrane potential-sensitive dyes and a nucleic acid marker is presented. The use of propidium iodide, rhodamine, and oxonol has facilitated the differentiation of cells of Escherichia coli and Salmonella typhimurium of various states of vitality following various treatments (heat, sonication, electroporation, and incubation with gramicidin) and during starvation in artificial seawater. The fluorescence intensity is directly correlated with viable cell counts for rhodamine 123 labelling, whereas oxonol and propidium iodide labelling is inversely correlated with viable counts. The distribution of rhodamine and oxonol uptake during starvation-survival clearly indicates that single-species starved bacteria are heterogeneous populations, and flow cytometry can be a fundamental tool for quantifying this heterogeneity.

The ability of membrane potential dyes and calcafluor white to distinguish between viable and non-viable bacteria.

Various dyes were assessed for their ability to discriminate between viable and non-viable bacteria. Two methods of killing were employed: by heat treatment or by gramicidin treatment. Staining was carried out in two ways; by staining directly in the medium or by washing cells prior to staining in buffer. Carbocyanine and rhodamine 123 dyes only exhibited small changes in fluorescence between viable and non-viable populations of bacteria. Both oxonol dye (bis 1,3-dibutylbarbituric acid trimethine oxonol) and calcafluor white proved much more useful.

Assessment of survival during starvation of Escherichia coli and Klebsiella pneumoniae in artificial urine: analysis of the kinetics of colony formation.

A kinetic model of colony formation was proposed by Hattori, based on a count of the colonies that appear on a plate in successive short intervals of time. In this model, three parameters (lambda, tr and N infinity) are defined, which reflect the ability of a bacterium to yield colonies and allow us to described the dynamics of bacterial populations in soil and of E. coli at different growth phases. In this paper we report a reparametrization of the kinetic model of colony formation, with the aim of facilitating more accurate calculation of lambda and tr. Moreover, we observed that during the starvation of E. coli and K. pneumoniae in urine, lambda can be used to assess survival, since this parameter clearly decreases during starvation. Retardation time values (tr) were similar in E. coli and K. pneumoniae throughout the starvation experimental period.

Variations in flow cytometric forward scatter signals and cell size in batch cultures of Escherichia coli.

Flow cytometry was used to study the lag, exponential, stationary and death phases of non-fixed cultures of Escherichia coli. Fluctuations in the forward angle scatter signal (FALS) were compared with cell size as measured by scanning electron microscopy at low temperature and image analysis. A correlation between FALS and cell size was not observed, although a correlation (r = -0.8) was obtained between FALS and the age of the culture for the first eleven days of incubation. Marked increases in FALS were observed during the lag phase, which were attributed both to changes in size and changes in structure or chemical composition. The distribution of FALS for all culture phases was asymmetric, and was associated with the cell size distribution.