Producción de bacillus thuringiensis H-14 var. israelensis utilizando espárrago (Asparagus officinalis) y su uso potencial para el control de la malaria en la Libertad - Perú / Bacillus thuringiensis H-14 var israelensis production using asparagus (Asparagus officinalis) and its potential use for Malaria control in la Libertad - Perú

Objetivo. Estandarizar un medio de cultivo utilizando la infusión de espárrago (Asparagus officinalis) para la producción masiva de bacillus thurningiensis (Bti) H-14 bvar. israelensis y determinar el efecto biolarvicida del Bti sobre Anopheles sp en criaderos naturales del distrito Laredo durante los meses de enero a diciembre del 2000. Materiales y métodos: Se ensayaron 3 medios a base de infusión de espárragos blanco: M1: 100 mL de la infusión, pH 9; M2: 50 mL de la infusión con 50 mL de buffer fosfato, pH 7; y M3: 25 mL de la infusión con 75 mL de agua destilada, pH 9. Como control se utilizó biolarvicidad a través del LC50 y LC90. El medio de cultivo óptimo (menor LC50 y LC90) sirvió para la producción masiva del Bti, el cual se sometió a bioensayo de laboratorio y aplicaciones en criaderos naturales. La efectividad fue determinada mediante la densidad larvaria pre y post aplicación del Bti. Resultados: El medio de cultivo óptimo para la producción de Bti fue M1, mostrando alta efectividad, con 100 por ciento de mortalidad en condiciones de laboratorio y 71-97 por ciento de mortalidad en el campo a las 24 horas de exposición con 3 aplicaciones realizadas semanalmente. Conclusiones: M1 es el medio óptimo para cultivar Bti, con alta efectividad para controlar larvas de Anopheles en el laboratorio y en el campo.

Objective: To standardize a culture medium using asparagus infusion (Asparagus officinalis) for the massive production of Bacillus thurigiensis (Bti) H-14 var. israelensis and to determine Bti bio-larvicide effect upon Anopheles sp. in natural breeding sites in Laredo district from January to December, 2000. Materials and methods: Three media based on white asparagus infusion were tested: M1: 100-mL infusion, pH: 9; M 2: 50-mL infusion plus 50-mL buffer phosphate, pH: 7, and M 3: 25-mL infusion plus 75-mL distilled water, pH: 9. The standard TPH medium was used as a control. Bti production in the different culture media was assessed in order to determine the bio-larvicide effectiveness using LC50 and LC90 The optimum culture medium (lower values for LC 50 and LC90) served for Bti production, and it was evaluated in laboratory and natural breeding sites. Effectiveness was determined measuring larval density prior and after Bti application. Results: The optimum medium for Bti production was M,. It showed high effectiveness, with 100% mortality under laboratory conditions, and 71-97% mortality in the field after 24 hours of exposition with three weekly applications. Conclusions: M1 is the optimum medium for culturing Bti, with high effectiveness for controlling Anopheles larvae both under laboratory and field conditions.

[Hematologic changes in anorexia nervosa]. / Las modificaciones hematológicas en la anorexia nerviosa.

We studied the hematologic values of 25 patients with anorexia nervosa. Age ranged from 12 to 38 years and 22 were females. Mean hematocrit was decreased to 36% and total leukocyte count to 4.400/microliters. Anemia was diagnosed in 13 patients. It was normocytic in 7 hypochromic microcytic in 7 and megaloblastic in 2. Seven of these anemic patients corrected their anemia with the treatment of anorexia nervosa, 3 required iron supplement and 2 folic acid. Bone marrow studies in 23 patients demonstrated hypoplasia in 17. Four patients had global bone marrow hypoplasia with decreased numbers of erythroblasts and megakaryocytes. A mucopolysaccharide substance substituted for a decreased fat content of bone marrow. A decreased bone marrow reserve of neutrophils and their margination in blood vessels contributed to leukopenia, which was not associated to an increased number of infections. Blood coagulation tests and platelet aggregation were normal.

Effect of theophylline on nuclear retention of oestrogenreceptor complexes: correlation with oestrogen responses.

In the ovariectomized adult rat uterine oedema induced by 0.01 and 0.1 micrograms oestradiol-17 beta/100g body weight increased further in the presence of theophylline. Nuclear retention of oestrogen-receptor complexes also increased in response to theophylline both in vivo and in vitro. Theophylline decreased the number of eosinophils in the blood and concurrently decreased oestrogen-induced uterine eosinophilia at doses of 0.001, 0.01, 0.1, 1, 10 or 30 micrograms oestradiol/100 g body weight, through a mechanism independent of glucocorticoids. There was, therefore, no correlation between changes in the number of uterine eosinophils and changes in uterine wet weight induced by theophylline and oestrogen. It is suggested that the presence of oestrogen-receptor complexes in the nucleus for at least 4 h is a prerequisite for the induction of uterine oedema and growth in the presence of theophylline and oestradiol-17 beta.

Efecto de inhibidores de la síntesis de prostaglandinas en el desarrollo embrionario del ratón / Effect of prostaglandins synthesis inhibitors on the embryonic development of mice

Se estudio el efecto de los inhibidores de la síntesis de prostaglandinas: naproxeno y sulindaco en un período crítico del desarrollo embrionario del ratón. Se analizaron las malformaciones obtenidas y se discutieron los mecanismos de acción de estos teratógenos