ABSTRACT
In a search for alternative, environmentally friendly and effective disinfecting agents, a commercially available protease-Neutrase® -was tested in this work for inactivation of koi herpesvirus (KHV) and of viral haemorrhagic septicaemia virus (VHSV). For comparison, the stability of these viral pathogens in similar configurations at various pH values and concentrations of peracetic acid or quicklime, typically used for disinfection, was tested. Therefore, virus suspensions were incubated with various concentrations of different agents for 24 hr and the titre of the remaining infectious particles was determined by virus titration. Furthermore, the treatment of both viruses, with the agents at concentrations that were previously appointed as effective, was also examined in the presence of solid material (quartz sand). All procedures investigated in this study, including the protease treatment, were able to reduce the titre of KHV and VHSV below the detection limit of the titration. Although further studies are necessary, this is the first report of the application of a protease for the inactivation of the selected fish pathogens, demonstrating the great potential of the latter for disinfection.
Subject(s)
Antiviral Agents/pharmacology , Herpesviridae/drug effects , Novirhabdovirus/drug effects , Animals , Fish Diseases/drug therapy , Fish Diseases/virology , Herpesviridae Infections/drug therapy , Herpesviridae Infections/veterinary , Herpesviridae Infections/virology , Peptide Hydrolases/pharmacology , Rhabdoviridae Infections/drug therapy , Rhabdoviridae Infections/veterinary , Rhabdoviridae Infections/virology , Viral Load/veterinaryABSTRACT
The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.
