1.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 62(Pt 6): 600-3, 2006 Jun 01.
Article
in English
| MEDLINE
| ID: mdl-16754993
ABSTRACT
For the first time, a non-catalytic and myotoxic Lys49-PLA2 (BthTX-I from Bothrops jararacussu venom) has been crystallized with BPB inhibitor. X-ray diffraction data were collected and electron-density calculations showed that the ligand is bound to the His48 residue. BthTX-I with His48 chemically modified by BPB shows strongly reduced myotoxic and cytotoxic activities. This suggests a biological correlation between the modification of His48, which is associated with catalytic activity of PLA2s, and other toxicological activities of Lys49-PLA2s.