BAP31 Plays an Essential Role in Mouse B Cell Development via Regulation of BCR Signaling.

B cell receptor-associated protein 31 (BAP31) is a transmembrane protein that is widely expressed and primarily located in the endoplasmic reticulum (ER). B cells play a crucial role in the immune system, and BAP31 significantly contributes to the functions of various immune cells. However, the specific role of BAP31 in B lymphocytes development remains unknown. In this study, we utilized a mouse model with BAP31 deleted from B cells to investigate its effects. Our findings reveal a block in early B cell development in the bone marrow and a significant decrease in the number of B cells in peripheral lymphoid organs taken from BAP31 B cell conditional knockout (BAP31-BCKO) mice. B cell receptor (BCR) signaling is crucial for the normal development and differentiation of B lymphocytes. BAP31, an endoplasmic reticulum membrane protein, directly regulates the BCR signaling pathway and was shown to be significantly positively correlated with B cell activation and proliferation. These findings establish BAP31 as a crucial regulator of early B cell development.

Affective norms for 501 Chinese words from three emotional dimensions rated by depressive disorder patients.

Introduction: Emotional words are often used as stimulus material to explore the cognitive and emotional characteristics of individuals with depressive disorder, while normal individuals mostly rate the scores of affective words. Given that individuals with depressive disorder exhibit a negative cognitive bias, it is possible that their depressive state could influence the ratings of affective words. To enhance the validity of the stimulus material, we specifically recruited patients with depression to provide these ratings. Methods: This study provided subjective ratings for 501 Chinese affective norms, incorporating 167 negative words selected from depressive disorder patients' Sino Weibo blogs, and 167 neutral words and 167 positive words selected from the Chinese Affective Word System. The norms are based on the assessments made by 91 patients with depressive disorder and 92 normal individuals, by using the paper-and-pencil quiz on a 9-point scale. Results: Regardless of the group, the results show high reliability and validity. We identified group differences in three dimensions: valence, arousal, and self-relevance: the depression group rated negative words higher, but positive and neutral words lower than the normal control group. Conclusion: The emotional perception affected the individual's perception of words, to some extent, this database expanded the ratings and provided a reference for exploring norms for individuals with different emotional states.

BAP31 Knockout in Macrophages Affects CD4+T Cell Activation through Upregulation of MHC Class II Molecule.

The differentiation of CD4+T cells is a crucial component of the immune response. The spleen and thymus, as immune organs, are closely associated with the differentiation and development of T cells. Previous studies have suggested that BAP31 may play a role in modulating T cell activation, but the specific impact of BAP31 on T cells through macrophages remains uncertain. In this study, we present evidence that BAP31 macrophage conditional knockout (BAP31-MCKO) mice display an enlarged spleen and thymus, accompanied by activated clustering and disrupted differentiation of CD4+T cells. In vitro co-culture studies were conducted to investigate the impact of BAP31-MCKO on the activation and differentiation of CD4+T cells. The examination of costimulatory molecule expression in BMDMs and RAW 264.7 cells, based on the endoplasmic reticulum function of BAP31, revealed an increase in the expression of antigen presenting molecules, particularly MHC-II molecule, in the absence of BAP31 in BMDMs or RAW264.7 cells. These findings suggest that BAP31 plays a role in the activation and differentiation of CD4+T cells by regulating the MHC class II molecule on macrophages. These results provide further support for the importance of BAP31 in developing interaction between macrophages and CD4+T cells.

Rapid and stable hydrogen detection based on Pd-modified WO3 nanosheets.

Materials with accurate and durable gas detection are essential for gas sensors. We developed a facile and effective method to deposit Pd onto WO3 nanosheets and used the samples for hydrogen gas sensing. The combination of the 2D ultrathin nanostructure of WO3 and the spillover effect of Pd enables a detection concentration down to 20 ppm of hydrogen and high selectivity against other gases including methane, butane, acetone, isopropanol, etc. Moreover, the durability of the sensing materials was testified through 50 cycles of exposure to 200 ppm of hydrogen. These outstanding performances are mainly attributed to a homogeneous and tenacious decoration of Pd on the surface of WO3 nanosheets, providing an attractive choice for practical applications.

Mapping distortion correction in off-axis aspheric mirror testing with a null compensator.

Mapping distortion can be introduced in null testing for off-axis aspheric surfaces, from which the testing results with coordinate deviation cannot be used to guide deterministic optical manufacturing. We propose a correction method for mapping distortion in this study based on the imaging distortion analysis of a null lens and iteration algorithm. We use imaging distortion data to fit the distortion polynomial function by the least-squares method, and we design an iteration algorithm to determine the rotation angle and the position of the null distortion point of the testing results. Finally, the mapping distortion can be accomplished without any markers on the mirror under test. The 280mm×180mm primary mirror and 234mm×176mm tertiary mirror of the hyperspectral camera were tested with a null compensator, and the distortion correction results were obtained using the proposed method. After magnetorheological finishing based on the correction results, the rms of the primary mirror and tertiary mirror reduced from 0.263λ to 0.013λ and 0.059λ to 0.018λ (λ=632.8nm), respectively.

Correlations between Clinical and Histopathologic Characteristics in Idiopathic Epiretinal Membrane.

PURPOSE: To investigate correlations between clinical and histopathologic characteristics of idiopathic epiretinal membrane (ERM). DESIGN: Retrospective interventional case series. PARTICIPANTS: In total, 87 eyes from 87 patients with idiopathic ERM who underwent pars plana vitrectomy with peeling of the ERM from 2019 to 2020 were included. METHODS: The outcomes of clinical ophthalmic examination, including measurement of best-corrected visual acuity (BCVA) and spectral-domain OCT (SD-OCT), before and after surgery were reviewed. Surgical specimens were fixed in formalin and embedded in paraffin for histologic and immunohistochemical analysis. MAIN OUTCOMES MEASURES: The association between morphological characteristics revealed on SD-OCT images and the cellular composition of the surgically excised ERM demonstrated with immunohistochemical staining were the main outcome measures. Changes in the BCVA and central macular thickness (CMT) were assessed through a comparison of preoperative and postoperative measurements. RESULTS: Based on SD-OCT morphological characteristics in the foveal area, 15 cases were classified into group 1A (mainly outer retinal thickening), 39 into group 1B (more tenting of the outer retina and distorted inner retina), and 33 into group 1C (prominent inner retina thickening). Overall, postoperative final BCVA and CMT at 1 year improved in all groups. Patients who presented with a better initial BCVA exhibited a more favorable final BCVA. Epiretinal membranes in group 1C demonstrated the greatest decrease in CMT compared with those in groups 1B and 1A, but the final CMT did not differ among the groups. A negative correlation between the density of hyalocytes (P = 0.003) and myofibroblasts (P = 0.047) was noted between the 3 groups. Total cell density and glial cell density of the ERMs were strongly associated with poor final BCVA and BCVA improvement. CONCLUSIONS: The present study provides new histopathologic information regarding the formation and progression of idiopathic ERM. Glial cell proliferation plays a predominant role in these processes. Epiretinal membranes with high cellularity and glial cell density may cause damage to the retina structure, resulting in poor postoperative visual outcomes. These findings provide additional evidence supporting early surgical intervention in patients with idiopathic ERM reported with visual disturbance.

Influencing Factors of Students Aged 10-20 Non-participating in Home Physical Exercise During the COVID-19 Isolation Policy Period: A Cross-Sectional Study From China.

Background: A number of public health measures are required during the COVID-19 pandemic. To stop the spread of COVID-19, the Chinese government has adopted isolation policies, including closing non-essential businesses, public transportation and schools, moving students' face-to-face learning to online, and recommending the cancellation of all non-essential activities and outdoor activities. However, while this isolation strategy has reduced human-to-human transmission of COVID-19, it has led to dramatic changes in students' daily lives and learning styles, including reduced physical activity and increased sedentary time. Considering the potentially harmful effects of physical inactivity, this study hoped to explore the incidence and influencing factors of non-participation in home physical exercise among Chinese students aged 10-20 during the implementation of the COVID-19 isolation policy. Methods: Through an online questionnaire platform, this study created an open-ended questionnaire (from March 1, 2020 to March 10, 2020) and distributed it to students in areas where isolation policies were enforced. The questionnaire was initially distributed by 10 recruited volunteers, and then the questionnaire was voluntarily forwarded and shared by the subjects or others, in a "snowball" way, to expand distribution. Finally, the survey data of 4,532 Chinese students aged 10-20 were collected. The incidence of respondents non-participating in home physical activity was determined using univariate analysis. Using odds ratios and 95% confidence intervals of a multivariate binary logistic regression model, factors influencing non-participation in home physical exercise were estimated. Results: Among the sample students, the incidence rate of non-participating in home physical exercise was 25.86% (24.06-27.15%). Exercise intentions, exercise habits, self-assessed health, beliefs in physical health, family exercise, family exercise recommendations, home exercise conditions, school exercise guidance, and health education programs had a negative impact on students non-participating in home physical exercise. Academic performance and electronic product use had a positive effect on non-participating in home physical exercise. Conclusions: A variety of forward leaning factors, enabling factors and demand factors have affected the occurrence of students" non-participating in home physical exercise. Future health isolation policies should take into account these influencing factors to reduce the occurrence of students" non-participating in home physical exercise and to promote students' independent participation in physical exercise.

Step-wise diagnostic approach for patients with uveitis - Experts consensus in Taiwan.

Uveitis is a sight-threatening disease that can be associated with many different etiologies. Successful treatment of uveitis relies on accurate diagnosis and prompt efficient therapy. History taking, physical and ocular examinations, systemic evaluations, and response to treatment provide crucial information to differentiate possible etiologies involved in the pathophysiology of intraocular inflammation. This article provides recommendations for a step-wise approach to patients with uveitis in Taiwan based on an expert meeting and consensus. Systemic evaluations for uveitis should be performed step-by-step and include investigation of patients' general systemic conditions, ruling out infectious etiologies, and obtaining evidential biomarkers to diagnose a specific disease entity.

Processing Self-Related Information Under Non-attentional Conditions Revealed by Visual MMN.

Mismatch negativity (MMN) of event-related potentials (ERPs) is a biomarker reflecting the preattentional change detection under non-attentional conditions. This study was performed to explore whether high self-related information could elicit MMN in the visual channel, indicating the automatic processing of self-related information at the preattentional stage. Thirty-five participants were recruited and asked to list 25 city names including the birthplace. According to the difference of relevance reported from the participants, we divided names of the different cities into high (birthplace as deviants), medium (Xi'an, where participants' university is located, as deviants), and low (totally unrelated cities as standard stimuli) self-related information. Visual MMN (vMMN) was elicited by high self-related information but not by medium self-related information, with an occipital-temporal scalp distribution, indicating that, under non-attentional condition, high self-related information can be effectively processed automatically in the preattentional stage compared with low self-related information. These data provided new electrophysiological evidence for self-related information processing.

Facile and robust fabrication of hierarchical Au nanorods/Ag nanowire SERS substrates for the sensitive detection of dyes and pesticides.

Surface enhanced Raman spectroscopy (SERS) has emerged as a promising tool for the rapid and ultrasensitive recognition of trace amounts of environmental pollutants. Hierarchical SERS substrates usually show superior performance to single-component substrates but require complicated preparation protocols. Herein, a facile, robust and low-cost route for the fabrication of hierarchical SERS substrates has been reported, in which no complicated laborious protocols or sophisticated equipment is needed. In the hierarchical SERS substrate, Au nanorods were distributed onto the network of Ag nanowires through evaporation induced self-assembly. The density of the Au nanorods and Ag nanowires could be easily tailored by tuning the number of droplets of gold nanorod solution and the concentration of silver nanowire solution. The nanogaps formed between Au nanorods and Ag nanowires were able to induce a rich enhanced electromagnetic field area via localized surface plasmon resonances and surface plasmon polaritons to achieve amplification of the Raman signal. The as-prepared substrate showed high uniformity and was capable of identifying 10-12 M rhodamine 6G, 10-10 M thiram and 10-10 M crystal violet, with correlation coefficients (R2) all higher than 0.98. This approach can be employed for the detection of trace dyes, pesticides or other environmental pollutants with high sensitivity and uniformity.

Visual outcomes and incidence of pseudophakic cystoid macular oedema in eyes with cataract and idiopathic epiretinal membrane after two-step sequential surgery.

OBJECTIVES: To determine whether pars plana vitrectomy (PPV) or cataract surgery should be performed first in concurrent epiretinal membrane (ERM) and cataract treatment with respect to visual outcome and pseudophakic cystoid macular oedema (PCMO) incidence. METHODS: Patients who underwent PPV and cataract surgery sequentially at a tertiary medical centre were retrospectively recruited. Best-corrected visual acuity (BCVA) and central macular thickness (CMT) measured from optical coherence tomography (OCT) images collected before and after each surgery were documented. OCT-defined PCMO incidence and its influence on visual outcomes were analysed. RESULTS: In total, 259 and 159 eyes received PPV (ERM-CATA) before and after cataract surgery (CATA-ERM), respectively. The ERM-CATA group had better final BCVA (logMAR: 0.274 vs. 0.558, p < 0.001) and greater BCVA gain (logMAR VA change: -0.379 vs. -0.220, p = 0.001) than did the CATA-ERM group. Baseline BCVA was positively correlated with final BCVA (p < 0.001), whereas baseline CMT, final CMT, and postoperative CMT changes were not. PCMO incidence did not differ significantly between the two groups (15.4% vs. 19.5%, p = 0.287), and final BCVA changes did not significantly differ between eyes with and without PCMO. PCMO incidence was much higher (29.40% vs. 16.30%, p = 0.008) in eyes with baseline CMT ≥ 500 µm. CONCLUSIONS: When managing ERM and cataract, PPV should be performed before cataract surgery to yield better visual outcomes. Both surgical sequences yield similar PCMO rates. Greater baseline CMT is a risk factor for PCMO after cataract surgery.

Escaping negative moods and concentration problems play bridge roles in the symptom network of problematic smartphone use and depression.

Background: Problematic smartphone use (PSU) has become an issue of great concern in the age of smartphones. PSU is associated with emotional problems, one of which is depression, as shown by empirical studies. However, previous studies have been limited in that they have focused solely on the total score for symptoms of PSU and depression while ignoring the symptomatic heterogeneity of these two concepts. Method: This study filled this gap by exploring links between symptoms of PSU and depression among 1,849 university students (59.17% female participants, 17-23 years old). Network analysis was utilized to reveal symptom connections, central symptoms, and bridge symptoms between PSU and depression. Results: (1) A total of 17 of 81 symptom connections (about 21%) between PSU and depression existed in the symptom network. For example, "self-control failure" for PSU was positively correlated with "concentration problems" for depression; (2) "recklessly continuing" for PSU and "fatigue" for depression were central symptoms within the PSU symptom network and depression symptom network, respectively; (3) "escaping negative moods" for PSU and "concentration problems" for depression were bridge symptoms. The former was maximumly connected with the depression symptoms and the latter was maximumly connected with the PSU symptoms; and (4) gender had very minimal influence on the network characteristics. Conclusion: The results are in keeping with the central idea of the compensatory internet use theory that excessive smartphone use may be a coping strategy for depressed emotions derived from escaping motivation. Moreover, concentration problems may be a mediator explaining how negative emotions (e.g., depression) cause PSU, which is undefined in current internet use theories. Finally, symptom connections, central symptoms, and bridge symptoms could be potential targets for the prevention and intervention of PSU and depression in young adults.

Aptamer-based biosensors for the diagnosis of sepsis.

Sepsis, the syndrome of infection complicated by acute organ dysfunction, is a serious and growing global problem, which not only leads to enormous economic losses but also becomes one of the leading causes of mortality in the intensive care unit. The detection of sepsis-related pathogens and biomarkers in the early stage plays a critical role in selecting appropriate antibiotics or other drugs, thereby preventing the emergence of dangerous phases and saving human lives. There are numerous demerits in conventional detection strategies, such as high cost, low efficiency, as well as lacking of sensitivity and selectivity. Recently, the aptamer-based biosensor is an emerging strategy for reasonable sepsis diagnosis because of its accessibility, rapidity, and stability. In this review, we first introduce the screening of suitable aptamer. Further, recent advances of aptamer-based biosensors in the detection of bacteria and biomarkers for the diagnosis of sepsis are summarized. Finally, the review proposes a brief forecast of challenges and future directions with highly promising aptamer-based biosensors.

Enhanced Eradication of Bacterial/Fungi Biofilms by Glucose Oxidase-Modified Magnetic Nanoparticles as a Potential Treatment for Persistent Endodontic Infections.

Bacterial/fungal biofilm-mediated persistent endodontic infections (PEIs) are one of the most frequent clinical lesions in the oral cavity, resulting in apical periodontitis and tooth damage caused by loss of minerals. The conventional root canal disinfectants are poorly bio-safe and harmful to teeth and tissues, making them ineffective in treating PEIs. The development of nanomaterials is emerging as a promising strategy to eradicate disease-related bacteria/fungi. Herein, glucose oxidase (GOx)-modified magnetic nanoparticles (MNPs) were synthesized via a facile and versatile route for investigating their effects on removing PEI-related bacterial/fungal biofilms. It is found that GOx was successfully immobilized on the MNPs by detecting the changes in the diameter, chemical functional group, charge, and magnetic response. Further, we demonstrate that GOx-modified MNPs (GMNPs) exhibit highly effective antibacterial activity against Enterococcus faecalis and Candida albicans. Moreover, the antibacterial/fungal activity of GMNPs is greatly dependent on their concentrations. Importantly, when placed in contact with bacterial/fungal biofilms, the dense biofilm matrix is destructed due to the movement of GMNPs induced by the magnetic field, the formation of reactive oxygen species, and nutrient starvation induced by GOx. Also, the in vitro experiment shows that the as-prepared GMNPs have excellent cytocompatibility and blood compatibility. Thus, GMNPs offer a novel strategy to treat bacteria/fungi-associated PEIs for potential clinical applications.

First Report of Anthracnose on Jerusalem Cherry Caused by Colletotrichum liaoningense in Shandong, China.

Jerusalem cherry (Solanum pseudocapsicum), which belongs to the genus Solanum and the family Solanaceae, possesses high ornamental value and is widely cultivated as an indoor ornament due to its bright red berries at maturity (Xu et al., 2018). In September 2019, leaf spot was detected on jerusalem cherry plants in Yuxiu Park, Shizhong district, Jinan, Shandong Province. Field surveys were done in a 1/15 ha park. Disease incidence was estimated at approximately 18% across the survey area. Foliar symptoms began as small white spots. As the disease progressed, lesions expanded and merged, and developed into large irregular white spots, with pale grey edge. At last, lesions were densely distributed throughout the leaves. To isolate the pathogen, twenty leaf tissues (5 × 5 mm) were cut from the border between diseased and healthy tissue, surface disinfected in 75% alcohol for 15 s, soaked in 0.1% mercuric chloride for 1 min, washed with sterile distilled water three times, and cultured on potato dextrose agar (PDA) at 25°C. Nineteen fungal isolates were obtained and were single-spored to obtain pure cultures. The colony of LCL7, a representative isolate, on PDA was initially white to orange, but turned black after 3 to 4 days incubation with black conidial masses. Conidia were single-celled, hyaline, straight, cylindrical, apex obtuse, and ranged from 13.4 to 18.3 × 3.2 to 4.9 µm (n = 50) （Diao et al., 2017）. To validate the species identification, rDNA internal transcribed spacer (ITS) region (White et al., 1990), and the partial sequences of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), ß-tubulin (TUB2), and chitin synthase (CHS-1) (Damm et al., 2019; He et al., 2019), were amplified and sequenced. The ITS, GAPDH, ACT, TUB2, and CHS-1 sequences of isolate LCL7 were submitted to GenBank (MW221320, MW227217, MW227218, MW227219, and MW266988, respectively). ITS, ACT, TUB2, and CHS-1 BLAST showed 99-100% homology with sequences of Colletotrichum liaoningense (ITS, 100% to MH636504; ACT, 100% to MH622582; TUB2, 99.56% to MH622714, CHS-1, 99.33% to MH622446, respectively), although GAPDH showed 93.98% homology with sequence MH681383 (234/249bp). Neighbor-joining tree based on concatenated sequences of the five genes was constructed using MEGA7.0. The results showed the isolate was closely related to C. liaoningense. Based on morphological and molecular characteristics, the isolate LCL7 was identified as C. liaoningense. Pathogenicity tests were performed by spraying a conidial suspension (1 × 105 conidia/mL) on ten two-year-old healthy jerusalem cherry plants. Ten other plants with sterile water served as controls. All samples were incubated in a growth chamber at 25±2°C and transparent plastic bags to keep relative humidity high for 2 days. All inoculated plants showed symptoms similar to those observed in the field after 21 days, but no disease occurred on control plants. The same fungus was successfully reisolated from inoculated leaves and reidentified based on morphology and molecular characteristics, and the fungus was not isolated from the control plants, thus confirming Koch's postulates. Pathogenicity tests were repeated twice. C. liaoningense can cause anthracnose in chili and mango in China (Diao et al., 2017; Li et al., 2019).To our knowledge, this is the first report of anthracnose on jerusalem cherry caused by C. liaoningense in China, which influences ornamental value and reduces market value. Identification of the causes of the disease will help develop effective strategies for managing this disease.

Deep learning and ensemble stacking technique for differentiating polypoidal choroidal vasculopathy from neovascular age-related macular degeneration.

Polypoidal choroidal vasculopathy (PCV) and neovascular age-related macular degeneration (nAMD) share some similarity in clinical imaging manifestations. However, their disease entity and treatment strategy as well as visual outcomes are very different. To distinguish these two vision-threatening diseases is somewhat challenging but necessary. In this study, we propose a new artificial intelligence model using an ensemble stacking technique, which combines a color fundus photograph-based deep learning (DL) model and optical coherence tomography-based biomarkers, for differentiation of PCV from nAMD. Furthermore, we introduced multiple correspondence analysis, a method of transforming categorical data into principal components, to handle the dichotomous data for combining with another image DL system. This model achieved a robust performance with an accuracy, sensitivity, specificity, and area under the receiver operating characteristic curve of 83.67%, 80.76%, 84.72%, and 88.57%, respectively, by training nearly 700 active cases with suitable imaging quality and transfer learning architecture. This work could offer an alternative method of developing a multimodal DL model, improve its efficiency for distinguishing different diseases, and facilitate the broad application of medical engineering in a DL model design.

Development of affinity between target analytes and substrates in surface enhanced Raman spectroscopy for environmental pollutant detection.

Environmental pollution has long been a social concern due to the variety of pollutants and their wide distribution, persistence and being detrimental to health. It is therefore necessary to develop rapid and sensitive strategies to trace and detect these compounds. Among various detection methodologies, surface enhanced Raman spectroscopy (SERS) has become an attractive option as it enables accurate analyte identification, simple sample preparation, rapid detection and ultra-high sensitivity without any interference from water. For SERS detection, an essential yet challenging step is the effective capture of target analytes onto the surface of metal nanostructures with a high intensity of enhanced electromagnetic field. This review has systematically summarized recent advances in developing affinity between targets and the surface of SERS substrates via direct adsorption, hydrophobic functional groups, boronate affinity, metal organic frameworks (MOFs), DNA aptamers and molecularly imprinted polymers (MIPs). At the end of this review, technical limitations and outlook have been provided, with suggestions on optimizing SERS techniques for real-world applications in environmental pollutant detection.

Flow signal change in polyps after anti-vascular endothelial growth factor therapy.

Optical coherence tomography angiography (OCTA) is a novel, non-invasive imaging tool used to detect vascular flow. The absence of a flow signal in OCTA in polyps revealed by indocyanine green angiography (ICGA) in patients with polypoidal choroidal vasculopathy (PCV) may indicate slow or compromised filling of blood flow from choroidal vessels. Naïve patients with PCV treated with intravitreal injections of aflibercept (IVI-A) were enrolled in this study to validate the hypothesis that baseline flow may affect the outcome of polyp regression in ICGA. The flow signal of polyps in OCTA was detected by manual segmentation in the corresponding location by ICGA. Polyps were defined as high-flow if both OCTA and ICGA showed positive findings, and low-flow if OCTA showed a negative flow signal in 3 consecutive horizontal scans at the polyp area shown in ICGA. A total of 24 polyps were identified in 13 PCV patients at baseline. Of these 24 polyps, 22 (91.7%) were high-flow and 2 (8.3%) were low-flow. After 3 monthly IVI-A, all low-flow polyps had complete regression in ICGA. Among 17 (77%) high-flow polyps at baseline that had regression after treatment, 10 (58.8%) became low-flow, while 5 (22.7%) persistent polyps remained high-flow. Flow signal of polyps as detected by OCTA could be a predictive factor for treatment response in patients with PCV. Monitoring changes in flow signal after treatment is clinically relevant.

The Nanoassembly of an Intrinsically Cytotoxic Near-Infrared Dye for Multifunctionally Synergistic Theranostics.

The combination of diagnostic and therapeutic functions in a single theranostic nanoagent generally requires the integration of multi-ingredients. Herein, a cytotoxic near-infrared (NIR) dye (IR-797) and its nanoassembly are reported for multifunctional cancer theranostics. The hydrophobic IR-797 molecules are self-assembled into nanoparticles, which are further modified with an amphiphilic polymer (C18PMH-PEG5000) on the surface. The prepared PEG-IR-797 nanoparticles (PEG-IR-797 NPs) possess inherent cytotoxicity from the IR-797 dye and work as a chemotherapeutic drug which induces apoptosis of cancer cells. The IR-797 NPs are found to have an ultrahigh mass extinction coefficient (444.3 L g-1 cm-1 at 797 nm and 385.9 L g-1 cm-1 at 808 nm) beyond all reported organic nanomaterials (<40 L g-1 cm-1 ) for superior photothermal therapy (PTT). In addition, IR-797 shows some aggregation-induced-emission (AIE) properties. Combining the merits of good NIR absorption, high photothermal energy conversion efficiency, and AIE, makes the PEG-IR-797 NPs useful for multimodal NIR AIE fluorescence, photoacoustic, and thermal imaging-guided therapy. The research exhibits the possibility of using a single ingredient and entity to perform multimodal NIR fluorescence, photoacoustic, and thermal imaging-guided chemo-/photothermal combination therapy, which may trigger wide interest from the fields of nanomedicine and medicinal chemistry to explore multifunctional theranostic organic molecules.

Correction: An, F., et al. A Conjugate of Pentamethine Cyanine and 18F as a Positron Emission Tomography/Near-Infrared Fluorescence Probe for Multimodality Tumor Imaging. Int. J. Mol. Sci. 2017, 18, 1214.

The authors wish to make the following corrections to this paper [...].