Subject(s)
Fluorescent Dyes , Hippocampus/chemistry , Photons , Zinc/analysis , Animals , Hippocampus/cytology , Microscopy/methods , Naphthalenes , RatsABSTRACT
We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 mum in live tissues using two-photon microscopy.
Subject(s)
Fluorescent Dyes/chemistry , Membrane Lipids/chemistry , Membrane Microdomains/chemistry , Naphthalenes/chemistry , Animals , Cells, Cultured , Fluorescence , Fluorescent Dyes/pharmacokinetics , Hippocampus/cytology , Hippocampus/drug effects , Hippocampus/metabolism , Macrophages/drug effects , Macrophages/metabolism , Membrane Microdomains/drug effects , Membrane Microdomains/metabolism , Mice , Molecular Structure , Organ Culture Techniques , Photons , Pyramidal Cells/drug effects , Pyramidal Cells/metabolism , RatsSubject(s)
Acids/chemistry , Fluorescent Dyes/chemistry , Photons , Animals , Cell Survival , Hippocampus/metabolism , Molecular Structure , Photochemistry , RatsABSTRACT
2-Acetyl-6-(dimethylamino)naphthalene-derived two-photon fluorescent Ca2+ probes (ACa1-ACa3) are reported. They can be excited by a 780 nm laser beam, show 23-50-fold enhancement in one- and two-photon excited fluorescence in response to Ca2+, emit fourfold stronger two-photon excited fluorescence than Oregon Green 488 BAPTA-1 upon complexation with Ca2+, and can selectively detect intracellular free Ca2+ ions in live cells and living tissues with minimum interference from other metal ions and membrane-bound probes. Moreover, these probes are capable of monitoring calcium waves at a depth of 120-170 microm in live tissues for 1100-4000 s using two-photon microscopy with no artifacts of photobleaching.
Subject(s)
Astrocytes/metabolism , Calcium Signaling/physiology , Calcium/pharmacokinetics , Fluorescent Dyes/pharmacokinetics , Animals , Astrocytes/drug effects , Calcium/chemistry , Calcium/radiation effects , Cells, Cultured , Fluorescent Dyes/chemistry , Fluorescent Dyes/radiation effects , Hippocampus/cytology , Hippocampus/physiology , Hydrogen-Ion Concentration , Hypothalamus/cytology , Hypothalamus/physiology , Lasers , Microscopy, Fluorescence/methods , Molecular Structure , Photons , Rats , Rats, Sprague-Dawley , Stereoisomerism , Time Factors , Tissue DistributionABSTRACT
We have newly fabricated a long one-dimensional (1D) mesoporous carbon nanofiber by using a mesoporous silica nanofiber template. The resulting mesoporous carbon nanofiber shows the unique mesoporous structure of circularly wound nanochannel alignment perpendicular to the long fiber axis and the high gas sorption property, which interestingly presents the p-type semiconducting behavior.
