ABSTRACT
Matrix metalloproteinases (MMPs), the key enzymes responsible for matrix degradation, are derived from polymorphonuclear leukocytes during the early stages of periodontitis. The aim of this study was planned to determine the levels of GCF (gingival crevicular fluid) matrix metalloproteinase-8 (MMP-8) and metalloproteinase-9 (MMP-9) patients with periodontitis and in healthy controls. Levels of crevicular MMP-8 and -9 were determined by ELISA in subjects with healthy without any periodontal disease (n = 10) and periodontitis (n = 10). Significantly higher crevicular MMP-8 and -9 were observed in cases of periodontitis compared to healthy adults. Crevicular MMP-8 and -9 may serve as biomarkers of periodontal disease and aid in early detection of periodontitis.
ABSTRACT
Objetivo: Determinar os níveis de matriz GCF metaloproteinases 8 (MMP-8) e metaloproteinase 9 (MMP-9) em indivíduos saudáveis e com periodontite.Método: Dez pacientes com doença peridontal e 10 sujeitos saudáveis compuseram a amostra. As análises foram realizadas no Jain Diagnostic Centre, Nova Déli, Índia. O consentimento informado foi obtidos para todos os sujeitos. Níveis presentes no sulco crevicular de MMP-8 e MMP-9 foram determinados por meio do ELISA em ambos os grupos. Dois examinadores analisaram os parâmetros clínicos periodontais e a saliva não estimulada foi coletada. A perda de inserção óssea clínica foi determinada por meio da mensuração dos sítios interproximais.Resultados: Valores significativamente elevador de MMP-* e MMP-9 foram diagnosticados nos portadores de periodontite quando comparado aos indivíduos saudáveis.Conclusão: A presença no fluido crevicular de MMP-8 e MMP-9 servem como biomarcadores da doença periodontal e auxiliam na detecção precoce da periodontite.
Subject(s)
Humans , Male , Female , Adult , Periodontal Diseases , Gingival Crevicular Fluid , Matrix Metalloproteinases , PeriodontitisABSTRACT
Matrix metalloproteinases (MMPs), the key enzymes responsible for matrix degradation, are derived from polymorphonuclear leukocytes during the early stages of periodontitis. The present study determined the levels of GCF matrix metalloproteinase-2 (MMP-2) and metalloproteinase-9 (MMP-9) and salivary MMP-8 in patients with gingivitis and periodontitis and in healthy controls. Levels of crevicular MMP-2, MMP-9 and salivary MMP-8 were determined by ELISA in subjects with healthy gingiva (n = 15), gingivitis (n = 18) and periodontitis (n = 20). Significantly higher salivary MMP-8 and crevicular MMP-9 were observed in cases of periodontitis compared to gingivitis and healthy adults. On the other hand, crevicular MMP-2 levels in periodontitis subjects were lower than those in gingivitis and healthy subjects. The three MMP levels were highly correlated to probing depth, and bleeding on probing. Salivary MMP-8, crevicular MMP- 2 and 9 may serve as biomarkers of periodontal disease and aid in early detection of periodontitis or gingivitis.
