ABSTRACT
Various clinical specimens were processed to find the prevalence rate of enterococci and to identify the species of clinical isolates of enterococci. Screening of various clinical specimens revealed that enterococci were prevalent in 2% of the total specimens, with urine and wound to be the major site of isolation. Conventional test scheme proposed by Facklam and Collins and commercially available systems Rapid ID 32 Strep (biomereiux) were successfully used to speciate enterococcal strains. Five species of enterococci were identified in the study from a set of 396 cultures, with E. faecalis (79.79%), and E. faecium (11.11%) predominating. E. hirae (3.03%), E. gallinarum (3.03%), and E. casseliflavus (3.03%), were the other members of Enterococcus species identified.
Subject(s)
Enterococcus/isolation & purification , Cross Infection/epidemiology , Cross Infection/microbiology , Enterococcus/pathogenicity , Enterococcus faecalis/isolation & purification , Enterococcus faecium/isolation & purification , Gram-Positive Bacterial Infections/epidemiology , Gram-Positive Bacterial Infections/microbiology , Hospitals, University , Humans , India/epidemiologyABSTRACT
BACKGROUND & OBJECTIVES: Beta haemolytic streptococci belonging to Lancefield group A, B, C and G cause a wide spectrum of clinical diseases. Hence there is a need for rapid and accurate typing of these strains. The present study was undertaken to evaluate the use of intact cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for rapid discrimination between strains of beta haemolytic streptococci. METHODS: Colonies of beta haemolytic streptococci were emulsified with chemical matrix on the sample slide, dried and analyzed by MALDI-TOF-MS. RESULTS: The reproducibility of results for all groups of beta haemolytic streptococci was good and spectra obtained for Lancefield group A, C and G streptococci showed discrimination between the groups on visual comparison. A finer difference in spectrum was observed among group A streptococci isolated from different locations at different periods of time. INTERPRETATION & CONCLUSION: MALDI-TOF-MS may be a potential tool in discriminating between strains of beta haemolytic streptococci, and also in the characterisation of untypable strains of group A streptococci.
Subject(s)
Mass Spectrometry/methods , Streptococcal Infections/diagnosis , Streptococcus/metabolism , Adolescent , Child , Escherichia coli/metabolism , Humans , Reproducibility of Results , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Streptococcal Infections/microbiology , Streptococcus/geneticsABSTRACT
Thirty-eight isolates of group A streptococci from patients with pharyngitis, 13 isolates from patients with pyoderma and 28 carrier strains were subjected to biotyping by carbohydrate fermentation tests and production of beta-glucuronidase. Biotype 10 was observed most frequently among clinical isolates and biotypes 3 and 4 were most common among carrier isolates.
Subject(s)
Carrier State/microbiology , Pharyngitis/microbiology , Pyoderma/microbiology , Streptococcal Infections/microbiology , Streptococcus pyogenes/classification , Adolescent , Bacterial Typing Techniques , Carbohydrate Metabolism , Child , Child, Preschool , Fermentation , Glucuronidase/biosynthesis , Humans , Streptococcus pyogenes/isolation & purification , Streptococcus pyogenes/metabolismABSTRACT
The M protein of group A Streptococcus (GAS) is the major virulence factor and is coded by the emm gene. The current serologic M typing methods are now being replaced by alternate means of M type deduction such as emm gene sequencing. This is the first report of emm types of GAS which are prevalent in south India. We found no marked preponderance of any single emm sequence among our clinical isolates with 11 emm sequences being present in 34 isolates.
