ABSTRACT
OBJETIVOS: Conocer las acciones del oxígeno a alta concentración en cámara hiperbárica (CH) sobre la expresión de genes relacionados con el metabolismo óseo en líneas celulares osteoblásticas y hueso trabecular humano. MATERIAL Y MÉTODOS: Se analizó la expresión diferencial de varios genes relacionados con el metabolismo óseo (SOST, RUNX2, MMP14, OPG, HIF-1α y SIRT1) en dos líneas celulares osteoblásticas humanas (Saos y Super-Saos) y en fragmentos de hueso trabecular humano sometidos a una, tres o cinco sesiones de CH (90 minutos, oxígeno 100%; 2,3 atmósferas). En cada experimento se utilizó un control que no recibió CH. RESULTADOS: No encontramos diferencias significativas tras la CH en la expresión de los genes estudiados, ni en las células ni en hueso trabecular. Solo en la línea celular Super-Saos la expresión de OPG tras 5 sesiones de CH descendió 6 veces con respecto a la del grupo control (2-∆Ct de 72; p = 0,01). CONCLUSIONES: El oxígeno a alta concentración en cámara hiperbárica no parece tener influencia en la expresión de genes relacionados con el metabolismo óseo
OBJECTIVES: To learn how high concentration in hyperbaric oxygen therapy (HBO) acts on the expression of genes relatedto bone metabolism in osteoblast cell lines and human trabecular bone. MATERIAL AND METHODS: The differential expression of several genes related to bone metabolism (SOST, RUNX2, MMP14,OPG, HIF‐1α and SIRT1) in two human osteoblastic cell lines (Saos and Super‐Saos) and in human trabecular bone fragments subjected to one, three or five HBO sessions (90 minutes, 100% oxygen; 2.3 atmospheres). In each experiment, acontrol that did not receive HBO was used. RESULTS: We did not find significant differences after HBO in the expression of the genes studied, neither in the cells nor in trabecular bone. Only in the Super‐Saos cell line the expression of OPG after 5 sessions of HBO decreased 6 times with respect to that of the control group (2‐∆CtCt of 72; p = 0.01). CONCLUSIONS: High concentration oxygen in the hyperbaric chamber (HC) does not seem to influence the expression of genes related to bone metabolism
Subject(s)
Humans , Hyperbaric Oxygenation , Cancellous Bone/metabolism , Gene Expression , Osteoblasts , Case-Control StudiesABSTRACT
The endoesophageal brachytherapy technique provides good results in the treatment of oesophageal cancer, when indicated. In a consensus meeting, the Spanish Brachytherapy Group of SEOR and the Spanish Society of Medical Physics (SEFM) agreed on the indications, dose, fractionation schedule, prescription and reporting to be performed in endoesophageal brachytherapy. The results of this consensus are presented here as recommendations for medical practice (AU)
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Subject(s)
Female , Humans , Male , Brachytherapy , Radiometry/methods , Esophageal Neoplasms/radiotherapy , Epithelial Cells/microbiology , Biomarkers/analysis , Cell Transformation, Neoplastic/pathology , Angioplasty , Palliative Care/methods , Laser Therapy , Esophagus , EsophagusABSTRACT
The endoesophageal brachytherapy technique provides good results in the treatment of oesophageal cancer, when indicated. In a consensus meeting, the Spanish Brachytherapy Group of SEOR and the Spanish Society of Medical Physics (SEFM) agreed on the indications, dose, fractionation schedule, prescription and reporting to be performed in endoesophageal brachytherapy. The results of this consensus are presented here as recommendations for medical practice.
