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1.
Scand J Rheumatol ; 46(3): 198-205, 2017 May.
Article in English | MEDLINE | ID: mdl-27379927

ABSTRACT

OBJECTIVES: Vitamin D modulates inflammation, and this may explain the observed associations between vitamin D status and disorders driven by systemic inflammation, such as coronary artery disease (CAD) and inflammatory rheumatic diseases (IRDs). The aims of this study were to assess vitamin D status in patients with CAD alone and in patients with CAD and IRD, and to explore potential associations between vitamin D status and the presence of mononuclear cell infiltrates (MCIs) in the aortic adventitia of these patients. METHOD: Plasma levels of 25-hydroxyvitamin D3 [(25(OH)D3] were determined by radioimmunoassay and 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] by enzyme immunoassay in the 121 patients from the Feiring Heart Biopsy Study (FHBS) who had available histology data on adventitial MCIs; 53 of these had CAD alone and 68 had CAD and IRD. RESULTS: In the crude analysis, vitamin D levels were similar in CAD patients with and without IRD. After adjustment for potential confounders, IRD was associated with an increase of 8.8 nmol/L [95% confidence interval (CI) 1.0-16.6; p = 0.027] in 25(OH)D3 and an increase of 18.8 pmol/L (95% CI 4.3-33.3; p = 0.012) in 1,25(OH)2D3, while MCIs in the aortic adventitia were associated with lower levels of 1,25(OH)2D3 (ß = -18.8, 95% CI -33.6 to -4.0; p = 0.014). CONCLUSIONS: IRD was associated with higher levels of both 25(OH)D3 and 1,25(OH)2D3. These findings argue against the hypothesis that patients with high systemic inflammatory burden (CAD+IRD) should have lower vitamin D levels than those with less inflammation (CAD only). Of note, when controlled for potential confounders, low 1,25(OH)2D3 levels were associated with adventitial aortic inflammation.


Subject(s)
Adventitia/immunology , Aorta/immunology , Calcifediol/blood , Calcitriol/blood , Coronary Artery Disease/blood , Leukocytes, Mononuclear/immunology , Rheumatic Diseases/blood , Adventitia/pathology , Aged , Aorta/pathology , Case-Control Studies , Coronary Artery Disease/complications , Coronary Artery Disease/immunology , Female , Humans , Leukocytes, Mononuclear/cytology , Linear Models , Male , Middle Aged , Multivariate Analysis , Radioimmunoassay , Rheumatic Diseases/complications , Rheumatic Diseases/immunology
2.
J Intern Med ; 273(5): 429-36, 2013 May.
Article in English | MEDLINE | ID: mdl-23600398

ABSTRACT

Exposure to environmental toxins is associated with a variety of age-related diseases including cancer and neurodegeneration. For example, in Parkinson's disease (PD), chronic environmental exposure to certain toxins has been linked to the age-related development of neuropathology. Neuronal damage is believed to involve the induction of neuroinflammatory events as a consequence of glial cell activation. Cellular senescence is a potent anti-cancer mechanism that occurs in a number of proliferative cell types and causes the arrest of proliferation of cells at risk of malignant transformation following exposure to potentially oncogenic stimuli. With age, senescent cells accumulate and express a senescence-associated secretory phenotype (SASP; that is the robust secretion of many inflammatory cytokines, growth factors and proteases). Whereas cell senescence in peripheral tissues has been causally linked to a number of age-related pathologies, little is known about the induction of cellular senescence and the SASP in the brain. On the basis of recently reported findings, we propose that environmental stressors associated with PD may act in part by eliciting senescence and the SASP within non neuronal glial cells in the ageing brain, thus contributing to the characteristic decline in neuronal integrity that occurs in this disorder.


Subject(s)
Aging , Cellular Senescence , Environmental Exposure/adverse effects , Environmental Pollutants/adverse effects , Neuroglia , Parkinson Disease/etiology , Parkinson Disease/pathology , Humans , Inflammation Mediators/metabolism , Neuroglia/pathology , Parkinson Disease/metabolism , Phenotype , Signal Transduction , Time Factors
3.
Waste Manag ; 32(1): 31-40, 2012 Jan.
Article in English | MEDLINE | ID: mdl-21975300

ABSTRACT

An environmental assessment of the management of organic household waste (OHW) was performed from a life cycle perspective by means of the waste-life cycle assessment (LCA) model EASEWASTE. The focus was on home composting of OHW in Denmark and six different home composting units (with different input and different mixing frequencies) were modelled. In addition, incineration and landfilling was modelled as alternatives to home composting. The most important processes contributing to the environmental impact of home composting were identified as greenhouse gas (GHG) emissions (load) and the avoided emissions in relation to the substitution of fertiliser and peat when compost was used in hobby gardening (saving). The replacement of fertiliser and peat was also identified as one of the most sensible parameters, which could potentially have a significant environmental benefit. Many of the impact categories (especially human toxicity via water (HTw) and soil (HTs)) were affected by the heavy metal contents of the incoming OHW. The concentrations of heavy metals in the compost were below the threshold values for compost used on land and were thus not considered to constitute a problem. The GHG emissions were, on the other hand, dependent on the management of the composting units. The frequently mixed composting units had the highest GHG emissions. The environmental profiles of the home composting scenarios were in the order of -2 to 16 milli person equivalents (mPE) Mg(-1) wet waste (ww) for the non-toxic categories and -0.9 to 28mPEMg(-1) ww for the toxic categories. Home composting performed better than or as good as incineration and landfilling in several of the potential impact categories. One exception was the global warming (GW) category, in which incineration performed better due to the substitution of heat and electricity based on fossil fuels.


Subject(s)
Garbage , Refuse Disposal/methods , Denmark , Environment , Incineration , Models, Chemical , Soil
4.
Waste Manag ; 31(9-10): 1934-42, 2011.
Article in English | MEDLINE | ID: mdl-21658932

ABSTRACT

A comprehensive experimental setup with six single-family home composting units was monitored during 1 year. The composting units were fed with 2.6-3.5 kg organic household waste (OHW) per unit per week. All relevant consumptions and emissions of environmental relevance were addressed and a full life-cycle inventory (LCI) was established for the six home composting units. No water, electricity or fuel was used during composting, so the major environmental burdens were gaseous emissions to air and emissions via leachate. The loss of carbon (C) during composting was 63-77% in the six composting units. The carbon dioxide (CO(2)) and methane (CH(4)) emissions made up 51-95% and 0.3-3.9% respectively of the lost C. The total loss of nitrogen (N) during composting was 51-68% and the nitrous oxide (N(2)O) made up 2.8-6.3% of this loss. The NH(3) losses were very uncertain but small. The amount of leachate was 130 L Mg(-1) wet waste (ww) and the composition was similar to other leachate compositions from home composting (and centralised composting) reported in literature. The loss of heavy metals via leachate was negligible and the loss of C and N via leachate was very low (0.3-0.6% of the total loss of C and 1.3-3.0% of the total emitted N). Also the compost composition was within the typical ranges reported previously for home composting. The level of heavy metals in the compost produced was below all threshold values and the compost was thus suitable for use in private gardens.


Subject(s)
Recycling , Soil/analysis , Gases/analysis , Organic Chemicals , Waste Products
5.
Neuroscience ; 172: 379-86, 2011 Jan 13.
Article in English | MEDLINE | ID: mdl-21055449

ABSTRACT

Parkinson's disease (PD) involves both motor and non-motor disturbances. Non-motor features include alterations in sensory olfactory function which may constitute a viable biomarker for the disorder. It is not clear what causes olfactory dysfunction but it appears to coincide with the development of synucleopathy within the olfactory bulb (OB). Elevation in alpha-synuclein (a-syn) is indeed a risk factor for development of the sporadic disorder. The multifactorial nature of the idiopathic disease combined with variability in its presentation suggests that it is likely to be influenced by several factors and that in vivo models that explore the synergistic effect of alpha-synuclein elevation with other potential contributing factors are likely to be of importance in understanding the disease etiology. Using a dual transgenic (DTg) mouse model of dopaminergic alpha-synuclein overexpression coupled with doxycycline (Dox)-inducible glutathione (GSH) depletion in these same cells, we demonstrate an age-related loss in behavioral olfactory function coupled with a significant neurodegeneration of glomerular dopaminergic neurons. This is accompanied by increase in alpha-synuclein levels in non-dopaminergic cells in the granule cell layer (GCL). In addition, isolated olfactory bulb synaptosomes from dual transgenic lines with Dox consistently showed a slight but significant reduction in maximum mitochondrial respiration compared to controls. These results suggest that in the presence of increased oxidative stress, increased alpha-synuclein expression within dopaminergic OB neurons results in neurodegeneration in the glomerular layer (GL) and increased alpha-synuclein levels in the granular cell layer which coincide with olfactory dysfunction.


Subject(s)
Aging/genetics , Dopamine/physiology , Glutathione/deficiency , Olfaction Disorders/genetics , Olfaction Disorders/physiopathology , Olfactory Bulb/physiopathology , alpha-Synuclein/genetics , Animals , Female , Male , Mice , Mice, Transgenic , Neurodegenerative Diseases/genetics , Neurodegenerative Diseases/metabolism , Neurodegenerative Diseases/physiopathology , Olfaction Disorders/metabolism , Olfactory Bulb/metabolism , Olfactory Bulb/pathology , Oxidative Stress/genetics , Parkinson Disease/genetics , Parkinson Disease/metabolism , Parkinson Disease/physiopathology , Up-Regulation/genetics , alpha-Synuclein/biosynthesis
6.
Waste Manag ; 30(12): 2475-82, 2010 Dec.
Article in English | MEDLINE | ID: mdl-20674324

ABSTRACT

The emission of greenhouse gases (GHGs) is a potential environmental disadvantage of home composting. Because of a lack of reliable GHG emission data, a comprehensive experimental home composting system was set up. The system consisted of six composting units, and a static flux chamber method was used to measure and quantify the GHG emissions for one year composting of organic household waste (OHW). The average OHW input in the six composting units was 2.6-3.5 kg week(-1) and the temperature inside the composting units was in all cases only a few degrees (2-10 °C) higher than the ambient temperature. The emissions of methane (CH(4)) and nitrous oxide (N(2)O) were quantified as 0.4-4.2 kg CH(4)Mg(-1) input wet waste (ww) and 0.30-0.55 kg N(2)OMg(-1)ww, depending on the mixing frequency. This corresponds to emission factors (EFs) (including only CH(4) and N(2)O emissions) of 100-239 kg CO(2)-eq.Mg(-1)ww. Composting units exposed to weekly mixing had the highest EFs, whereas the units with no mixing during the entire year had the lowest emissions. In addition to the higher emission from the frequently mixed units, there was also an instant release of CH(4) during mixing which was estimated to 8-12% of the total CH(4) emissions. Experiments with higher loads of OHW (up to 20 kg every fortnight) entailed a higher emission and significantly increased overall EFs (in kg substance per Mg(-1)ww). However, the temperature development did not change significantly. The GHG emissions (in kg CO(2)-eq.Mg(-1)ww) from home composting of OHW were found to be in the same order of magnitude as for centralised composting plants.


Subject(s)
Air Pollutants/analysis , Garbage , Recycling , Waste Management , Air Pollution/statistics & numerical data , Carbon Dioxide/analysis , Carbon Monoxide/analysis , Greenhouse Effect , Housing/statistics & numerical data , Methane/analysis , Nitrogen Dioxide/analysis , Soil/analysis , Soil/chemistry
7.
J Hand Surg Eur Vol ; 34(4): 475-8, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19675027

ABSTRACT

The results in 75 of 105 patients with Older type II/III (AO type A2.2, A3.1, A3.2) Colles' fractures, treated with non-bridging external fixation are presented. The mean age was 67.8 years, and all patients were followed prospectively for 12 months with radiological and functional assessment. No statistically significant loss of radial length, angulation or inclination was seen between the postoperative reduction and the 1-year follow-up examination. The clinical results after 1 year were 66 (88%) excellent/good, nine (12%) fair and 0 (0%) poor according to the modified Gartland and Werley score. Mean visual analogue scale pain score after 1 year was 0.8. In three patients (4%), re-displacement of the fracture occurred and was treated with plating. Non-bridging external fixation offers a reliable method of maintaining radiological reduction of Older type II/III fractures of the distal radius and gives a good functional outcome after 1 year.


Subject(s)
Colles' Fracture/surgery , External Fixators , Fracture Healing/physiology , Postoperative Complications/diagnostic imaging , Adult , Aged , Aged, 80 and over , Bone Nails , Colles' Fracture/diagnostic imaging , Female , Follow-Up Studies , Humans , Male , Middle Aged , Pain Measurement , Patient Satisfaction , Prospective Studies , Radiography , Range of Motion, Articular/physiology
8.
Neuroscience ; 153(3): 664-70, 2008 May 15.
Article in English | MEDLINE | ID: mdl-18407421

ABSTRACT

In response to injury, endogenous precursors in the adult brain can proliferate and generate new neurons, which may have the capacity to replace dysfunctional or dead cells. Although injury-induced neurogenesis has been demonstrated in animal models of stroke, Alzheimer's disease (AD) and Huntington's disease (HD), studies of Parkinson's disease (PD) have produced conflicting results. In this study, we investigated the ability of adult mice to generate new neurons in response to the parkinsonian toxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), which causes selective degeneration of nigrostriatal dopamine neurons. MPTP lesions increased the incorporation of 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdU), as well as the number of cells that co-expressed BrdU and the immature neuronal marker doublecortin (DCX), in two neuroproliferative regions-the subgranular zone of the dentate gyrus (DG) and the rostral subventricular zone (SVZ). BrdU-labeled, DCX-expressing cells were not found in the substantia nigra (SN) of MPTP-treated mice, where neuronal cell bodies are destroyed, but were present in increased numbers in the striatum, where SN neurons lost in PD normally project. Fibroblast growth factor-2 (FGF-2), which enhances neurogenesis in a mouse model of HD, also increased the number of BrdU/DCX-immunopositive cells in the SN of MPTP-treated mice. Thus, MPTP-induced brain injury increases striatal neurogenesis and, in combination with FGF-2 treatment, also stimulates neurogenesis in SN.


Subject(s)
Corpus Striatum/cytology , Fibroblast Growth Factor 2/pharmacology , Neurogenesis/drug effects , Parkinsonian Disorders/physiopathology , Substantia Nigra/cytology , Animals , Corpus Striatum/drug effects , Corpus Striatum/metabolism , Doublecortin Protein , Fluorescent Antibody Technique , Mice , Parkinsonian Disorders/metabolism , Substantia Nigra/drug effects , Substantia Nigra/metabolism
9.
Neuroscience ; 149(4): 917-30, 2007 Nov 23.
Article in English | MEDLINE | ID: mdl-17936517

ABSTRACT

UNLABELLED: Oxidative/nitrosative stress and mitochondrial dysfunction have been implicated in the degeneration of dopaminergic neurons in the substantia nigra during Parkinson's disease (PD). During early stages of PD, there is a significant depletion of the thiol antioxidant glutathione (GSH), which may lead to oxidative stress, mitochondrial dysfunction, and ultimately neuronal cell death. Mitochondrial complex I (CI) is believed to be the central player to the mitochondrial dysfunction occurring in PD. We have generated a dynamic, mechanistic model for mitochondrial dysfunction associated with PD progression that is activated by rotenone, GSH depletion, increased nitric oxide and peroxynitrite. The potential insults independently inhibit CI and other complexes of the electron transport chain, drop the proton motive force, and reduce ATP production, ultimately affecting the overall mitochondrial performance. We show that mitochondrial dysfunction significantly affects glutathione synthesis thereby increasing the oxidative damage and further exacerbating the toxicities of these mitochondrial agents resulting in neurodegeneration. Rat dopaminergic neuronal cell culture and in vitro experiments using mouse brain mitochondria were employed to validate important features of the model. MAJOR CONCLUSIONS: Using a combination of experimental and in silico modeling approaches, we have demonstrated the interdependence of mitochondrial function with GSH metabolism in relation to neurodegeneration in PD.


Subject(s)
Glutathione/metabolism , Mitochondria/pathology , Models, Neurological , Neurons , Nonlinear Dynamics , Adenosine Triphosphate/metabolism , Analysis of Variance , Animals , Cell Line, Transformed , Cell Survival , Dose-Response Relationship, Drug , Drug Interactions , Electron Transport Complex III/metabolism , Enzyme Inhibitors/pharmacology , Glutathione Synthase/metabolism , Mice , Mitochondria/drug effects , Mitochondria/metabolism , NAD/metabolism , Neurons/metabolism , Neurons/pathology , Neurons/ultrastructure , Rats , Reactive Oxygen Species/metabolism , Rotenone/pharmacology
10.
Exp Neurol ; 192(1): 203-14, 2005 Mar.
Article in English | MEDLINE | ID: mdl-15698635

ABSTRACT

Glutathione peroxidase (GSHPx) has been demonstrated in several in vivo studies to reduce both the risk and severity of oxidatively-induced tissue damage. The seizure-inducing neurotoxin kainic acid (KA) has been suggested to elicit its toxic effects in part via generation of oxidative stress. In this study, we report that expression of elevated levels of murine GSHPx-1 in transgenic mice surprisingly results in increased rather than decreased KA susceptibility including increased seizure activity and neuronal hippocampal damage. Isolated transgenic primary hippocampal culture neurons also display increased susceptibility to KA treatment compared with those from wildtype animals. This could be due to alterations in the redox state of the glutathione system resulting in elevated glutathione disulfide (GSSG) levels which, in turn, may directly activate NMDA receptors or enhanced response of the NMDA receptor.


Subject(s)
Epilepsy/enzymology , Genetic Predisposition to Disease/genetics , Glutathione Peroxidase/genetics , Hippocampus/enzymology , Nerve Degeneration/enzymology , Oxidative Stress/genetics , Animals , Cell Death/genetics , Cells, Cultured , Disease Models, Animal , Epilepsy/genetics , Epilepsy/physiopathology , Glutathione Disulfide/metabolism , Hippocampus/drug effects , Hippocampus/physiopathology , Kainic Acid/pharmacology , Mice , Mice, Transgenic , N-Methylaspartate/pharmacology , Nerve Degeneration/genetics , Nerve Degeneration/physiopathology , Neurons/drug effects , Neurons/metabolism , Neurons/pathology , Neurotoxins/pharmacology , Organ Culture Techniques , Oxidative Stress/drug effects , Receptors, N-Methyl-D-Aspartate/drug effects , Receptors, N-Methyl-D-Aspartate/metabolism , Up-Regulation/drug effects , Up-Regulation/physiology
11.
Neurotoxicology ; 23(4-5): 479-86, 2002 Oct.
Article in English | MEDLINE | ID: mdl-12428720

ABSTRACT

Oxidative stress is believed to play a key role in the degeneration of dopaminergic neurons in the substantia nigra (SN) of Parkinson's disease (PD) patients. An important biochemical feature of PD is a significant early depletion in levels of the thiol antioxidant compound glutathione (GSH) which may lead to the generation of reactive oxygen species (ROS), mitochondrial dysfunction, and ultimately to subsequent neuronal cell death. In earlier work from our laboratory, we demonstrated that depletion of GSH in dopaminergic PC12 cells affects mitochondrial integrity and specifically impairs the activity of mitochondrial complex I. Here we report that pre-treatment of PC12 cells with R-lipoic acid acts to prevent depletion of GSH content and preserves the mitochondrial complex I activity which normally is impaired as a consequence of GSH loss.


Subject(s)
Antioxidants/therapeutic use , Glutathione/physiology , Parkinson Disease/drug therapy , Thioctic Acid/therapeutic use , Animals , Buthionine Sulfoximine/pharmacology , Cytoplasm/drug effects , Cytoplasm/metabolism , Glutamate-Cysteine Ligase/metabolism , Glutathione/deficiency , Mitochondria/drug effects , Mitochondria/metabolism , PC12 Cells , Rats
12.
Neurology ; 59(5): 729-34, 2002 Sep 10.
Article in English | MEDLINE | ID: mdl-12221165

ABSTRACT

OBJECTIVE: Transgenic mice that overexpress a human gene encoding mutant cytosolic superoxide dismutase (SOD1) develop a progressive motor neuron loss that resembles human ALS. Why mutant SOD1 initiates motor neuron death is unknown. One hypothesis proposes that the mutant molecule has enhanced peroxidase activity, reducing hydrogen peroxide (H2O2) to form toxic hydroxyl adducts on critical targets. To test this hypothesis, the authors generated transgenic ALS mice with altered levels of glutathione peroxidase (GSHPx), the major soluble enzyme that detoxifies H2O2. METHODS: SOD1(G93A) ALS mice were bred with mice bearing a murine GSHPx transgene that have a four-fold elevation in brain GSHPx levels and with mice having targeted inactivation of the GSHPx gene and reduced brain GSHPx activity. RESULTS: Survival was not prolonged in ALS mice with elevated brain GSHPx activity (p = 0.09). ALS mice with decreased GSHPx brain activity (20% of normal) showed no acceleration of the disease course (p = 0.89). The age at disease onset in the ALS mice was unaffected by brain GSHPx activity. CONCLUSION: The level of GSHPx activity in the CNS of transgenic ALS mice does not play a critical role in the development of motor neuron disease.


Subject(s)
Amyotrophic Lateral Sclerosis/metabolism , Brain/enzymology , Glutathione Peroxidase/genetics , Glutathione Peroxidase/metabolism , Age of Onset , Amyotrophic Lateral Sclerosis/mortality , Animals , Female , Genotype , Male , Mice , Mice, Transgenic , Phenotype , Superoxide Dismutase/genetics , Superoxide Dismutase/metabolism , Superoxide Dismutase-1 , Survival Analysis
13.
Vet Rec ; 150(25): 782-4, 2002 Jun 22.
Article in English | MEDLINE | ID: mdl-12135073

ABSTRACT

This paper summarises information on the current inspection procedures for pig heads on the slaughterline and their impact on food safety, and considers the implications for food safety of certain lesions. It is argued that although a modified slaughter and inspection technique would decrease the contamination of the carcase with pathogenic microorganisms, leaving lesions in the head undiscovered would be of little or no importance either for human health or for the overall supervision of animal health.


Subject(s)
Abattoirs/standards , Bacterial Infections/prevention & control , Food Microbiology , Head/microbiology , Meat/microbiology , Animals , Bacterial Infections/epidemiology , Europe/epidemiology , Humans , Prevalence , Swine
14.
J Neurosci ; 21(24): 9519-28, 2001 Dec 15.
Article in English | MEDLINE | ID: mdl-11739563

ABSTRACT

Parkinson's disease (PD) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) toxicity are both associated with dopaminergic neuron death in the substantia nigra (SN). Apoptosis has been implicated in this cell loss; however, whether or not it is a major component of disease pathology remains controversial. Caspases are a major class of proteases involved in the apoptotic process. To evaluate the role of caspases in PD, we analyzed caspase activation in MPTP-treated mice, in cultured dopaminergic cells, and in postmortem PD brain tissue. MPTP was found to elicit not only the activation of the effector caspase-3 but also the initiators caspase-8 and caspase-9, mitochondrial cytochrome c release, and Bid cleavage in the SN of wild-type mice. These changes were attenuated in transgenic mice neuronally expressing the general caspase inhibitor protein baculoviral p35. These mice also displayed increased resistance to the cytotoxic effects of the drug. MPTP-associated toxicity in culture was found temporally to involve cytochrome c release, activation of caspase-9, caspase-3, and caspase-8, and Bid cleavage. Caspase-9 inhibition prevented the activation of both caspase-3 and caspase-8 and also inhibited Bid cleavage, but not cytochrome c release. Activated caspase-8 and caspase-9 were immunologically detectable within MPP(+)-treated mesencephalic dopaminergic neurons, dopaminergic nigral neurons from MPTP-treated mice, and autopsied Parkinsonian tissue from late-onset sporadic cases of the disease. These data demonstrate that MPTP-mediated activation of caspase-9 via cytochrome c release results in the activation of caspase-8 and Bid cleavage, which we speculate may be involved in the amplification of caspase-mediated dopaminergic cell death. These data suggest that caspase inhibitors constitute a plausible therapeutic for PD.


Subject(s)
Carrier Proteins/metabolism , Caspases/metabolism , Parkinson Disease, Secondary/enzymology , 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine , Animals , BH3 Interacting Domain Death Agonist Protein , Caspase 3 , Caspase 8 , Caspase 9 , Cell Count , Cells, Cultured , Cytochrome c Group/metabolism , Disease Models, Animal , Enzyme Activation/drug effects , Enzyme Inhibitors/metabolism , Enzyme Inhibitors/pharmacology , Humans , Inhibitor of Apoptosis Proteins , Mesencephalon/cytology , Mesencephalon/drug effects , Mesencephalon/metabolism , Mice , Mice, Transgenic , Mitochondria/metabolism , Neurons/cytology , Neurons/drug effects , Neurons/metabolism , PC12 Cells , Parkinson Disease, Secondary/chemically induced , Parkinson Disease, Secondary/pathology , Parkinson Disease, Secondary/prevention & control , Rats , Substantia Nigra/drug effects , Substantia Nigra/enzymology , Substantia Nigra/pathology , Tyrosine 3-Monooxygenase/metabolism , Viral Proteins/genetics , Viral Proteins/metabolism , Viral Proteins/pharmacology
15.
Mol Biotechnol ; 19(1): 45-57, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11697220

ABSTRACT

Genetically engineered animal models have been and will continue to be invaluable for exploring the basic mechanisms involved in the aging process as well as in extending our understanding of diseases found to be more prevalent in the older human population. Continued development of such in vivo systems will allow scientists to further dissect the role genetic and environmental factors play in aging and in age-related disease states and to enhance our understanding of these processes. In this article we discuss techniques involved in the development of such models and review some examples of laboratory mouse strains that have been used to study either normal aging or select diseases associated with aging.


Subject(s)
Aging , Mice, Mutant Strains , Aging/genetics , Aging/immunology , Aging/psychology , Alzheimer Disease , Animals , Cellular Senescence , Gene Targeting/methods , Genetic Engineering , Humans , Mice , Mice, Knockout , Mice, Transgenic , Parkinson Disease , Research , Transgenes
16.
Mech Ageing Dev ; 122(14): 1499-510, 2001 Sep 30.
Article in English | MEDLINE | ID: mdl-11511392

ABSTRACT

Protein aggregation appears to be the common denominator in a series of distinct neurodegenerative diseases yet its role in the associated neuronal pathology in these various conditions remains elusive. In Parkinson's disease, localization of alpha synuclein aggregates within intracellular Lewy body occlusions represent a major hallmark of this disorder and suggest that such aggregation may play a causative role in the resulting dopaminergic cell loss. In this Viewpoint article, recent data is reviewed related to how alpha synuclein aggregation may occur, what cellular events might be responsible, and how this may interfere with normal cellular function(s). It appears likely that while aggregation of alpha synuclein may interfere with its normal function in the cell, this is not the primary cause of the related neurodegeneration.


Subject(s)
Nerve Tissue Proteins/metabolism , Parkinson Disease/metabolism , Animals , Humans , Mutation , Nerve Tissue Proteins/genetics , Parkinson Disease/genetics , Synucleins , alpha-Synuclein
17.
Bioessays ; 23(7): 640-6, 2001 Jul.
Article in English | MEDLINE | ID: mdl-11462217

ABSTRACT

Recently it has been hypothesized that apoptotic cell death is involved in several neuropathological conditions including Parkinson's disease (PD). Initial morphological studies assessing the presence of apoptosis in Parkinsonian brain tissues yielded mixed results. Based on more recent studies in human PD brains as well in animal and cell culture models of the disease, a picture is emerging, however, that strongly suggests that many of the molecular players thought to participate in this type of neuronal cell death are active in the disease. The task of researchers in the field is now to deduce how these players may be interacting with one another to bring about cell death in PD and to design effective therapies to interfere with these processes.


Subject(s)
Apoptosis , Neurons/cytology , Parkinson Disease/pathology , Animals , Brain/pathology , Cell Culture Techniques , Disease Models, Animal , Glutathione/metabolism , Humans , Mitochondria , Parkinson Disease/therapy
18.
J Neurosci Res ; 64(2): 203-6, 2001 Apr 15.
Article in English | MEDLINE | ID: mdl-11288148

ABSTRACT

Glutathione (GSH) is considered one of the primary antioxidant compounds in the brain, important for the removal of peroxides from this organ. GSH levels have been reported to be significantly lower in the substantia nigra (SN) of Parkinson patients vs. age-matched controls. Curiously, GSH has been proposed to be present in brain astrocytes rather than in neurons even though these cells are not lost in Parkinson disease. We report that the catalytic and regulatory subunit proteins of glutamyl cysteine synthetase (GCS), the primary enzyme involved in GSH synthesis, are present not only in astrocytes but also in dopaminergic neurons of the SN. This may have important implications in terms of GSH loss associated with Parkinson disease.


Subject(s)
Astrocytes/enzymology , Dopamine/analysis , Glutamate-Cysteine Ligase/analysis , Glutathione/biosynthesis , Nerve Tissue Proteins/analysis , Neurons/enzymology , Substantia Nigra/enzymology , Allosteric Site , Animals , Catalytic Domain , Glutamate-Cysteine Ligase/chemistry , Mice , Mice, Inbred C57BL , Microscopy, Fluorescence , Nerve Tissue Proteins/chemistry , Parkinson Disease/enzymology , Protein Subunits , Substantia Nigra/cytology , Tyrosine 3-Monooxygenase/analysis
19.
J Neurochem ; 76(6): 1745-55, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11259492

ABSTRACT

Exposure of neurons to H(2)O(2) results in both necrosis and apoptosis. Caspases play a pivotal role in apoptosis, but exactly how they are involved in H(2)O(2)-mediated cell death is unknown. We examined H(2)O(2)-induced toxicity in neuronal PC12 cells and the effects of inducible overexpression of the H(2)O(2)-scavenging enzyme catalase on this process. H(2)O(2) caused cell death in a time- and concentration-dependent manner. Cell death induced by H(2)O(2) was found to be mediated in part through an apoptotic pathway as H(2)O(2)-treated cells exhibited cell shrinkage, nuclear condensation and marked DNA fragmentation. H(2)O(2) also triggered activation of caspase 3. Genetic up-regulation of catalase not only significantly reduced cell death but also suppressed caspase 3 activity and DNA fragmentation. While the caspase 3 inhibitor DEVD inhibited both caspase 3 activity and DNA fragmentation induced by H(2)O(2) it did not prevent cell death. Treatment with the general caspase inhibitor ZVAD, however, resulted in complete attenuation of H(2)O(2)-mediated cellular toxicity. These results suggest that DNA fragmentation induced by H(2)O(2) is attributable to caspase 3 activation and that H(2)O(2) may be critical for signaling leading to apoptosis. However, unlike inducibly increased catalase expression and general caspase inhibition both of which protect cells from cytotoxicity, caspase 3 inhibition alone did not improve cell survival suggesting that prevention of DNA fragmentation is insufficient to prevent H(2)O(2)-mediated cell death.


Subject(s)
Apoptosis/physiology , Caspases/metabolism , Cysteine Proteinase Inhibitors/pharmacology , DNA Fragmentation/physiology , Hydrogen Peroxide/toxicity , Neurons/drug effects , Animals , Apoptosis/drug effects , Caspase 3 , Caspase Inhibitors , Catalase/metabolism , Cell Death/drug effects , Cell Death/physiology , Cell Survival/drug effects , DNA Fragmentation/drug effects , Enzyme Activation , Glutathione/metabolism , Neurons/cytology , Neurons/physiology , PC12 Cells , Rats , Recombinant Fusion Proteins/metabolism , Signal Transduction , Trans-Activators/metabolism , Transfection
20.
Novartis Found Symp ; 235: 11-20; discussion 20-5, 2001.
Article in English | MEDLINE | ID: mdl-11280021

ABSTRACT

A growing body of evidence has implicated oxidative stress as an important factor in the neuropathology associated with Parkinson's disease. Dopaminergic nigrostriatal neurons, the predominant cells lost in Parkinson's, are believed to be highly prone to oxidative damage due to the propensity for dopamine to auto-oxidize and thereby produce elevated levels of hydrogen peroxide and catecholamine quinones. Hydrogen peroxide formed during this process can either be converted by iron to form highly reactive hydroxyl radicals or removed through reduction by glutathione. Glutathione can also conjugate with quinones formed during dopamine oxidation preventing them from facilitating the release of iron from the iron-storage molecule ferritin. Alterations in both iron and glutathione levels in the substantia nigra have been correlated with the neuronal degeneration accompanying Parkinson's disease but a direct causative role for either has yet to be definitively proved. We will discuss the use of genetically engineered cell and mouse lines generated in our laboratory as models to examine the role that alterations in iron and glutathione levels may play in neurodegeneration of dopaminergic neurons of the substantia nigra associated with Parkinson's disease, and how these two parameters may interact with one another to bring this about.


Subject(s)
Glutathione/metabolism , Iron/metabolism , Parkinson Disease/pathology , Animals , Glutathione/physiology , Humans , Iron/physiology , Mice , Parkinson Disease/metabolism
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