Ancient Sheep Genomes Reveal Four Millennia of North European Short-Tailed Sheep in the Baltic Sea Region.

Sheep are among the earliest domesticated livestock species, with a wide variety of breeds present today. However, it remains unclear how far back this diversity goes, with formal documentation only dating back a few centuries. North European short-tailed (NEST) breeds are often assumed to be among the oldest domestic sheep populations, even thought to represent relicts of the earliest sheep expansions during the Neolithic period reaching Scandinavia <6,000 years ago. This study sequenced the genomes (up to 11.6X) of five sheep remains from the Baltic islands of Gotland and Åland, dating from the Late Neolithic (∼4,100 cal BP) to historical times (∼1,600 CE). Our findings indicate that these ancient sheep largely possessed the genetic characteristics of modern NEST breeds, suggesting a substantial degree of long-term continuity of this sheep type in the Baltic Sea region. Despite the wide temporal spread, population genetic analyses show high levels of affinity between the ancient genomes and they also exhibit relatively high genetic diversity when compared to modern NEST breeds, implying a loss of diversity in most breeds during the last centuries associated with breed formation and recent bottlenecks. Our results shed light on the development of breeds in Northern Europe specifically as well as the development of genetic diversity in sheep breeds, and their expansion from the domestication center in general.

Ancient DNA from a 2700-year-old goitered gazelle (Gazella subgutturosa) supports gazelle hunting in Iron Age Central Asia.

Central Asia has been an important region connecting the different parts of Eurasia throughout history and prehistory, with large states developing in this region during the Iron Age. Archaeogenomics is a powerful addition to the zooarchaeological toolkit for understanding the relation of these societies to animals. Here, we present the genetic identification of a goitered gazelle specimen (Gazella subgutturosa) at the site Gazimulla-Tepa, in modern-day Uzbekistan, supporting hunting of the species in the region during the Iron Age. The sample was directly radiocarbon dated to 2724-2439 calBP. A phylogenetic analysis of the mitochondrial genome places the individual into the modern variation of G. subgutturosa. Our data do represent both the first ancient DNA and the first nuclear DNA sequences of this species. The lack of genomic resources available for this gazelle and related species prevented us from performing a more in-depth analysis of the nuclear sequences generated. Therefore, we are making our sequence data available to the research community to facilitate other research of this nowadays threatened species which has been subject to human hunting for several millennia across its entire range on the Asian continent.

Reductive evolution of virulence repertoire to drive the divergence between community- and hospital-associated methicillin-resistant Staphylococcus aureus of the ST1 lineage.

Methicillin-resistant Staphylococcus aureus (MRSA) of the ST1-SCCmecIV lineage has been associated with community-acquired (CA) infections in North America and Australia. In Brazil, multi-drug resistant ST1-SCCmecIV MRSA has emerged in hospital-associated (HA) diseases in Rio de Janeiro. To understand these epidemiological differences, genomic and phylogenetic analyses were performed. In addition, virulence assays were done for representative CA - and HA-MRSA strains. Despite the conservation of the virulence repertoire, some genes were missing in Brazilian ST1-SCCmecIV including lukSF-PV, fnbB, and several superantigen-encoded genes. Additionally, CA-MRSA lost the splDE while HA-MRSA strains conserved the complete operon. Most of these variable genes were located in mobile genetic elements (MGE). However, conservation and maintenance of MGEs were often observed despite the absence of their associated virulence markers. A Bayesian phylogenetic tree revealed the occurrence of more than one entrance of ST1 strains in Rio de Janeiro. The tree shape and chronology allowed us to infer that the hospital-associated ST1-SCCmecIV from Brazil and the community-acquired USA400 from North America are not closely related and that they might have originated from different MSSA strains that independently acquired SCCmecIV cassettes. As expected, representatives of ST1 strains from Brazil showed lower cytotoxicity and a greater ability to survive inside human host cells. We suggest that Brazilian ST1-SCCmecIV strains have adapted to the hospital setting by reducing virulence and gaining the ability to persist and survive inside host cells. Possibly, these evolutionary strategies may balance the biologic cost of retaining multiple antibiotic resistance genes.

O encontro de Piaget com o teste de inteligência de Cyril Burt: uma revisão narrativa / Piaget meeting with Cyril Burt's intelligence test: a narrative review / El encuentro de Piaget con el test de inteligencia de Cyril Burt: una revisión narrativa

O presente estudo tem por objetivo principal desenvolver uma narrativa que mostre a influência do teste de inteligência de Cyril Burt na criação do método clínico de Jean Piaget, auxiliando na composição de conhecimentos deste assunto pouco estudado. Piaget estudou em Neuchâtel, Zurique e Paris antes de integrar o Instituto Jean-Jacques Rousseau em Genebra, no qual lançou seus primeiros livros, que o levaram a lugar de destaque na psicologia mundial. O presente trabalho faz uma comparação entre os dados disponíveis na literatura para recompor essa trajetória até o ponto decisivo de mudança no trabalho experimental de Piaget, que aconteceu em Paris, onde estudou o teste de inteligência de Cyril Burt. Conhecer os elementos da criação do Método Clínico é importante para compreender os fundamentos ontológicos e epistemológicos dos dados gerados por ele e suas consequências teóricas. Do contato com esse teste, Piaget sofreu três impactos determinantes para sua vida e obra. A primeira está ligada à tradução do teste, que ressaltou questões sobre parte/todo na linguagem das crianças. O segundo é a possibilidade de Piaget de observar dezenas de amostras do raciocínio explicado das crianças por elas mesmas, o que permitiu o ingresso das técnicas de observação da zoologia em sua psicologia. Por fim, as possibilidades de modificação e adaptação do teste, sua primeira experiência original em psicologia experimental, técnica que carregaria sua vida toda na constituição de seu Método Clínico.

The main objective of this study is to present a narrative that shows the influence of Cyril Burt's intelligence test in the creation of the clinical method used Jean Piaget, helping in the composition of knowledge on this subject. Piaget studied in Neuchâtel, Zurich and Paris before joining the Jean-Jacques Rousseau Institute in Geneva, where he launched his first books that took him to a prominent place in the field of psychology. The present work makes a comparison between the data available in the literature to recompose this trajectory until the turning point in Piaget's experimental work, which happened in Paris, where he studied the Cyril Burt's intelligence test. Knowing the elements of the creation of the Clinical Method is important to understand the ontological and epistemological foundations of the data generated by this method and its theoretical consequences. From contact with Cyril Burt's test, Piaget suffered three determinant impacts on his life and work. The first is linked to the translation of the test, which highlighted questions about the notions of part-whole in the children's language. The second is the possibility for Piaget to observe dozens of samples of the children's reasoning explained by themselves, which allowed the introduction of zoology observation techniques in his psychology. Finally, the possibilities of modifying and adapting the test, which is his first original experience in experimental psychology, a technique that he would carry his entire life in the constitution of his Clinical Method.

El objetivo principal de este estudio es desarrollar una narrativa que muestre la influencia del test de inteligencia de Cyril Burt en la creación del método clínico de Jean Piaget, ayudando en la composición del conocimiento acerca deste tema poco comprendido. Piaget estudió en Neuchâtel, Zurich y París antes de unirse al Instituto Jean-Jacques Rousseau en Ginebra, donde lanzó sus primeros libros que lo llevaron a un lugar destacado en la psicología mundial. El presente trabajo hace una comparación entre los datos disponibles en la literatura especializada para recomponer esta trayectoria hasta el punto de inflexión en el trabajo experimental de Piaget, que sucedió en París, donde estudió el test de inteligencia de Cyril Burt. Conocer los elementos de la creación del Método Clínico es importante para comprender tanto sus fundamentos ontológicos y epistemológicos cuanto los datos generados por él y sus consecuencias teóricas. El contacto con este test, Piaget sufrió tres impactos determinantes en su vida y su trabajo. El primero está vinculado a la traducción del test, donde analizó las preguntas sobre parte / todo en el idioma de los niños. La segunda es la oportunidad para Piaget de observar docenas de muestras del razonamiento de los niños explicadas por ellos mismos, lo que permitió la introducción de las técnicas de observación de zoología en su psicología. Finalmente, las posibilidades de modificar y adaptar el test, su primera experiencia original en psicología experimental, una técnica que llevaría toda su vida en la constitución de su Método Clínico.

Metal-Free Photoinduced Hydroalkylation Cascade Enabled by an Electron-Donor-Acceptor Complex.

A metal- and photocatalyst-free photoinduced radical cascade hydroalkylation of 1,7-enynes has been disclosed. The process is triggered by a single electron transfer (SET) event involving a photoexcited electron-donor-acceptor complex between an NHPI ester and a Hantzsch ester, which decomposes to afford a tertiary radical that is readily trapped by the enyne. The method provides an operationally simple, robust, and step-economical approach toward the construction of diversely functionalized dihydroquinolinones bearing quaternary centers. A sequential one-pot hydroalkylation-isomerization approach is also offered, giving access to a family of quinolinones. A wide substrate scope and high functional group tolerance were observed in both approaches.

Problemas de epistemologia entre Piaget e Maturana / Epistemology problems between Piaget and Maturana / Problemas de epistemología entre Piaget y Maturana

A psicologia possui várias matrizes diferentes, o que possibilitou a formação de diferentes paradigmas e interpretações. As ciências biológicas e naturais, com suas técnicas e descobertas, ofereceram à nascente ciência psicológica várias alternativas e matrizes teóricas. Este trabalho propõe uma comparação crítica entre duas linhas teóricas da psicologia, que são duas explicações epistemológicas de origens semelhantes, mas com pressupostos e conclusões bastante distintas: a Epistemologia Genética de Jean Piaget e a teoria da Autopoiese de Humberto Maturana. Para tanto alguns elementos de ambas as teorias são destacados em partes separadas para, ao final, concluir criticamente sobre as semelhanças e diferenças de ambas. As principais diferenças entre ambas residem na natureza da relação sujeito/objeto, nos pressupostos do funcionamento dos seres vivos e na ontologia.(AU)

Psychology has several different matrices, which allowed the formation of different paradigms and interpretations. Biological and natural sciences, with their techniques and findings, offered to the nascent Psychological Science various alternatives and theoretical nuances. This paper proposes a critical comparison of two theoretical lines of psychology, which are two epistemological explanations of similar backgrounds, but with very different assumptions and conclusions: the Genetic Epistemology by Jean Piaget and the theory of Autopoiesis of Humberto Maturana. Therefore some elements of both theories are highlighted in separate parts for, in the end, critically conclude about the similarities and differences of both. The main differences between the two lies on the nature of the subject / object, the premises of the functioning of living beings and ontology.(AU)

La psicología tiene variadas matrices diferentes, lo que permitió la formación de diferentes paradigmas e interpretaciones. Las ciencias biológicas y naturales, con sus técnicas y hallazgos, ofrecieron a la naciente ciencia psicológica varias alternativas y matices teóricos. Este artículo propone una comparación crítica entre dos líneas teóricas de psicología, que son dos explicaciones epistemológicas fundadas en antecedentes científicos similares, pero con supuestos y conclusiones muy diferentes: la Epistemología Genética de Jean Piaget y la teoría de la Autopoiesis de Humberto Maturana. Por lo tanto, algunos elementos de ambas teorías se destacan en partes separadas para, al final, concluir críticamente sobre las similitudes y diferencias de ambas. Las principales diferencias entre los dos encuentra-se en la naturaleza del sujeto / objeto, las premisas del funcionamiento de los seres vivos y la ontología.(AU)

Comparative mitogenomic analyses of Amazona parrots and Psittaciformes.

Amazon parrots are long-lived birds with highly developed cognitive skills, including vocal learning. Several parrot mitogenomes have been sequenced, but important aspects of their organization and evolution are not fully understood or have limited experimental support. The main aim of the present study was to describe the mitogenome of the blue-fronted Amazon, Amazona aestiva, and compare it to other mitogenomes from the genus Amazona and the order Psittaciformes. We observed that mitogenomes are highly conserved among Amazon parrots, and a detailed analysis of their duplicated control regions revealed conserved blocks. Population level analyses indicated that the specimen analyzed here seems to be close to A. aestiva individuals from Bahia state. Evolutionary relationships of 41 Psittaciformes species and three outgroups were inferred by BEAST. All relationships were retrieved with high support.

Evaluation of the reproducibility of the AO/ASIF classification for humeral shaft fractures.

OBJECTIVE: To evaluate the reproducibility of the AO/Asif classification for humeral shaft fractures. METHODS: Consecutive radiographs of the arm in both anteroposterior and lateral view from 60 patients with humeral shaft fractures were analyzed. Six observers who were familiar with the AO/Asif classification (three shoulder and elbow surgery specialists and three general orthopedists) were selected to make the analysis, which was done at three different times. The data were subjected to statistical analysis using the kappa coefficient. RESULTS: The intra and interobserver concordance was statistically significant in all the analyses. CONCLUSIONS: All the evaluators showed concordance between the three evaluations that was considered to be statistically significant. However, the highest values were found among the specialists.

OBJETIVO: Avaliar a reprodutibilidade da classificação AO/Asif para as fraturas diafisárias do úmero. MÉTODOS: Foram analisadas radiografias consecutivas em duas incidências (anteroposterior e perfil do braço) de 60 pacientes com fratura do úmero diafisário. Seis observadores familiarizados com a classificação AO/Asif, três especialistas em cirurgia do ombro e cotovelo e três ortopedistas gerais foram selecionados para análise, a qual se deu em três tempos distintos. Os dados foram submetidos à análise estatística com o coeficiente kappa (κ). RESULTADOS: A concordância intra e interobservadores foi estatisticamente significante em todas as análises. CONCLUSÕES: Todos os avaliadores concordam com as três avaliações consideradas estatisticamente significantes. Porém, os maiores valores são encontrados entre os especialistas.

Avaliação da reprodutibilidade da classificação AO/Asif para fraturas diafisárias do úmero / Evaluation of the reproducibility of the AO/ASIF classification for humeral shaft fractures

Avaliar a reprodutibilidade da classificação AO/Asif para as fraturas diafisárias do úmero. MÉTODOS: Foram analisadas radiografias consecutivas em duas incidências (anteroposterior e perfil do braço) de 60 pacientes com fratura do úmero diafisário. Seis observadores familiarizados com a classificação AO/Asif, três especialistas em cirurgia do ombro e cotovelo e três ortopedistas gerais foram selecionados para análise, a qual se deu em três tempos distintos. Os dados foram submetidos à análise estatística com o coeficiente kappa(κ). RESULTADOS: A concordância intra e interobservadores foi estatisticamente significante em todas as análises. CONCLUSÕES: Todos os avaliadores concordam com as três avaliações consideradas estatisticamente significantes. Porém, os maiores valores são encontrados entre os especialistas.

To evaluate the reproducibility of the AO/Asif classification for humeral shaft fractures. METHODS: Consecutive radiographs of the arm in both anteroposterior and lateral view from 60 patients with humeral shaft fractures were analyzed. Six observers who were familiar with the AO/Asif classification (three shoulder and elbow surgery specialists and three general orthopedists) were selected to make the analysis, which was done at three different times. The data were subjected to statistical analysis using the kappa coefficient. RESULTS: The intra and interobserver concordance was statistically significant in all the analyses. CONCLUSIONS: All the evaluators showed concordance between the three evaluations that was considered to be statistically significant. However, the highest values were found among the specialists.

Avaliação dos resultados do mini-lifting modificado: estudo prospectivo / Modified minilifting results: prospective study

Introdução: O mini-lifting modificado se baseia em uma técnica padronizada, que consiste em um descolamento mínimo demarcado no pré-operatório. A plicatura do sistema músculo-aponeurótico superficial (SMAS) é realizada tracionando-se todos os tecidos da face, a fim de promover o seu rejuvenescimento com a manutenção de um retalho que apresenta menor risco de necrose por ter menor descolamento e, por isso, uma mínima área para formação de hematomas. Método: Foram operadas 36 pacientes submetidas a ritidoplastia pela técnica do mini-lifting modificado, que preencheram critérios pré-estabelecidos. Foram coletados os dados antropométricos e um interrogatório sobre a história clínica do paciente realizado. Resultados: No período de fevereiro de 2008 a março de 2009, foram operadas consecutivamente 36 pacientes pela técnica de mini-lifting modificado. A idade variou entre 44 e 67 anos, sendo a média de 57 anos. O retorno das pacientes às atividades habituais variou de 7 a 14 dias, período que coincide com a redução em mais de 80% do edema pós-operatório. O dreno a vácuo foi retirado na manhã seguinte em 34 (94,4%) pacientes, no 2º dia pós-operatório em 1 (2,94%) caso e houve 1 (2,94%) caso de hematoma pós-operatório. Não houve caso de necrose do retalho. Houve 1 (2,94%) caso de neuropraxia do facial, que foi resolvida espontaneamente. Conclusão: A técnica de mini-lifting modificado foi considerada satisfatória, por apresentar bons resultados, baixo índice de complicações e retorno precoce das pacientes às suas atividades.

Introduction: The modified mini-face lift is based on a standardized tecnic consisting of a minimum undermining marked preoperatively. The plication of the muscle-aponeurotic superficial (SMAS) is done pulling all the tissues of the face, promoting facial rejuvenation with the maintenance of a flap with a lower risk of necrosis by maintaining smaller undermining area and minimum area for hematomas. Methods: We operated on 36 patients under going rhytidectomy technique of modified mini-lifting as they fulfilled the some criteria. Anthropometric data were collected, a questionnaire about the patient’s history wasper formed. Results: From February 2008 to March 2009, 36 patients were consecutively operated by the modified mini-facelift technique. The age ranged from 44 to 67 years, with a mean 57 anos. The patients return to normal activities ranged from 7 to 14 days, a period that coincides with a reduction in more than 80% of post operative edema. The vacuum drain was removed the next morning (1st day after surgery) in 34 patients (94.4%), the 2nd post operative day in 1 (2.94%) case and in 1 (2.94%) case of post operative hematoma. There were no cases of necrosis of the flap. There was 1 (2.94%) neuropraxia facial which was resolved spontaneously. Conclusion: The technique of modified mini-lifting is satisfactory for presenting good results, low complication rate and early return of patients to their activities.