Isolamento no território federal do Amapá de um vírus nôvo relacionado sorològicamente aos agentes responsáveis pelas febres hemorrágicas da Argentina e da Bolívia / Isolation in the Federal Territory of Amapá of a new virus serologically related to the agents responsible for hemorrhagic fever in Argentina and Bolivia

Um nôvo membro do grupo Tacaribe, o vírus Amapari, tem sido inoculado repetidas vêzes das vísceras e sangue de Oryzomys e Neacomys, capturados em SN, Território Federal do Amapá. Um único isolamento foi obtido da urina das crias e de ectoparasitos dêsses animais. Os autores analisam algumas implicações ecológicas, bem como do ponto de vista de patogenicidade humana do referido agente. Referem, ainda, a importância da prova de FC’ como recurso de grande valor no isolamento dêste vírus, uma vez que os camundongos inoculados com os espécimens provenientes de animais infectados freqüentemente desenvolvem infecção inaparente.

A new member of the Tacaribe group, Amapari virus, has been repeatedly isolated from the viscera and blood of rodents belonging to the genera Oryzomys and Neacomys captured in the Serra do Navio region, Federal Territory of Amapá, Brazil. Single isolations were also obtained from the urine, young, and ectoparasites of these animals. The authors discuss certain aspects of the epizootiology of the above mentioned virus, with special reference to its pathogenicity in man. They also refer the importance of FC’ test as a tool of great value for isolation of this virus, since mice inoculated with specimens obtained from infected animals frequently develop inapparent infection.

Recuperacion de virus amarilico, procedente de un mono centinela, en las cercanias de Belém, Brasil¨

El virus de la Fiebre amarilla fue aislado de un mono centinela Cebus el 4 de diciembre de 1964 en el bosque Utinga, cercano a Belém, Brasil. De 30 monos silvestres, dez tenían anticuerpos del mencionado virus. Más de 1.200 muestras procedentes de otros vertebrados fueron comprobadas, sin que se obtuviera indicio alguno de relación con el virus amarílico. No se descubrieron casos humanos. El episodio hubiera quedado ignorado de no haberse ilevado a cabo una vigilancia habitual con respecto a los arbovirus.

Yellow fever virus was isolated from a sentinel Cebus monkey on 4 December 1964 in the Utinga forest near Belém, Brazil. Ten of 30 wild monkeys had Yellow fever antibody. Over 1,200 samples from other vertebrates were tested without evidence of involvement with Yellow fever virus. No human cases were found. The episode would have gone undetected if routine surveillance for arboviruses had not been in progress.

O vírus da Febre amarela foi isolado de um macaco sentinela Cebus em 4 de dezembro de 1964 no bosque Utinga, próximo a Belém, Brasil. De 30 macacos silvestres, dez tinham anticorpos da Febre amarela. Mais de 1.200 amostras procedentes de outros vertebrados foram examinadas sem que se obtivesse indício algum de relação com o vírus da doença. Não foram descobertos casos humanos. O episódio foi descoberto graças à vigilância habitual sobre os arbovírus.

Pacui virus, phlebotomine flies, and small mammals in Brazil: an epidemiological study.

Pacui virus, originally obtained from forest rodents, was isolated 100 times from 61,437 specimens (658 pools) of the phlebotomine fly Lutzomyia flaviscutellata, collected from rodent-baited traps in the forests of Belem, Para, Brazil in the period October 1968 through September 1970. Isolations were made from engorged and unengorged females and from males (3 strains), and occurred in all 24 months. Pacui virus also was isolated from the blood of two wild rodents (Oryzomys), but not from 424 L. infraspinosa, 12,000 mosquitoes, or sentinel mice. Pacui virus neutralizing antibodies were detected in serum of six bait animals after exposure to biting flies in the forest, in 30% of wild rodents surveyed (including two from Amapa Territory), and in 10% of marsupials, but were absent in human survey sera and in bats. Low-passage Pacui virus produced viremia in and was lethal to infant mice by the subcutaneous route. L. flaviscutellata was most abundant in the dry season, in which period Pacui virus isolations increased. This fly is strongly attracted to rodents close to the ground. L. flaviscutellata also yielded single strains of Guama, Icoaraci, and BeAr 177325 viruses.

Restan virus, a new group C arbovirus from Trinidad and Surinam

Restan virus is a new serotype of arbovirus group C that has been isolated seven times in Trinidad and Surinam during 1963 and 1964. Three strains were recovered from Culex spp. and four from acutely ill human beings. The new agent is related most closely to Marituba and Murutucu viruses, from which it is distinguishable only in hemagglutination-inhibitation test. It circulates to high titer in laboratory-colonized rodents of the species Zygodontomys b. brevicauda and Oryzomys laticeps velutinus. In the laboratory it has been transmitted by Aedes aegypti. Results of serum surveys are reported. (AU)