ABSTRACT
This study evaluated the influence of hypothalamic-pituitary-adrenal (HPA) axis in cutaneous wounds subjected to laser biomodulation. A total of 48 rats were divided into two groups: Group I (GI) with 24 adrenalectomized animals and Group II (GII) with 24 non-adrenalectomized animals. Each group was divided into two subgroups: the irradiated subgroup which laser was applied to four points at the edges of the wound (670 nm laser, 9 mW) and control subgroup. Rats in each subgroup were sacrificed at 24 or 72 h. Adrenal glands were only removed from GI rats. Three days after adrenalectomy, a cutaneous wound was made. An immunohistochemical analysis was performed using anti-CD45 and anti-CD8 antibodies. Flow cytometry was used to count T lymphocytes and their subpopulations in blood. Decreases in the number of CD45-positive inflammatory cells and in the total numbers of CD8- and CD45-positive cells were observed in histological sections of adrenalectomized animals subjected to laser biomodulation at 24h. Similar results were observed for distribution of total lymphocytes in blood (p<0.05). The action of 670 nm laser does not depend exclusively on HPA axis. It is believed that corticosteroid-promoting enzymes liberated in non-adrenal tissues may influence immune response under the influence of this type of phototherapy.
Subject(s)
Adrenal Glands/physiopathology , Hypothalamo-Hypophyseal System/physiopathology , Laser Therapy , Skin/injuries , Skin/physiopathology , Adrenal Glands/surgery , Adrenalectomy/adverse effects , Animals , CD4-Positive T-Lymphocytes/cytology , CD4-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/cytology , CD8-Positive T-Lymphocytes/immunology , Inflammation/blood , Inflammation/immunology , Inflammation/physiopathology , Inflammation/surgery , Male , Rats , Rats, Wistar , Skin/immunologyABSTRACT
Na LCL existe uma elevada resposta imune celular, e isto pode estar à presença de clones bastante reativos, sendo sua identificação muito relevante. Uma das abordagens para avaliar o papel de agentes infecciosos sobre o sistema imune é a análise do padrão dos receptores antigênicos expressos pelos linfócitos que participam ativamente das respostas específicas contra estes agentes. Técnicas imunológicas como a citometria de fluxo, tornam exeqüivel examinar a expressão de genes variáveis α e ß dos receptores antigênicos de células T. Na LV existe uma ausência de resposta imune celular que pode ser atribuída a deleção de determimados clones de células T, como pela diminuição na expressão de ligantes e moléculas de adesão ou apoptose nas células responsivas. Neste trabalho tivemos como objetivo avaliar os mecanismos imunológicos relacionados a hiperativação celular que é observada na leishmaniose cutânea localizada e a anergia que é observada na leishmaniose visceral (...)
