Mannosylerythritol lipids: production, downstream processing, and potential applications.

Mannosylerythritol lipids (MELs) are biosurfactants produced by various fungal species. Depending on the degree of acetylation and further chemical modifications, these glycolipids can show remarkable biological properties, including the increase of water retention in the stratum corneum suppression of melanogenic enzymes tyrosinase-1 and -2, reversion of UV-A radiation-induced aquaporin-3 suppression, skin whitening, and anti-aging effects. These applications of MELs require high purity, which is usually reached by liquid-liquid extraction followed by chromatography, obtaining ≥95% purity. This worked aimed to critically discuss the current state of the art and trends on the production of MELs, including post-production treatment as enzymatic conversion. In addition, their application as skincare or pharmaceutical agents and agricultural biostimulants.

Lipid and protein fingerprinting for Fusarium oxysporum f. sp. cubense strain-level classification.

Banana is one of the most popular fruits in the world but has been substantially impaired by Panama disease in the last years. Fusarium oxysporum f. sp. cubense (Foc) is the causal agent and colonizes banana cultivars from many subgroups with different aggressiveness levels, often leading to plant death while compromising new crops in infested areas. This study has evaluated the ability of MALDI-MS protein and lipid fingerprinting to provide intraspecies classification of Foc isolates and to screen biomolecules related to host-pathogen relationship. The MS data, when inspected via partial least square discriminant analysis (PLS-DA), distinguished the isolates by aggressiveness as well as by specific location and host. Although both lipids and proteins show discriminating tendencies, these differences were more clearly perceived via the protein profiles. Considering that Cavendish cultivar is the more resistant option to endure Foc presence in the field, the lipids and proteins related to this subgroup might have an important role in pathogen adaptation. This study reports a new application of MALDI-MS for the analysis of a banana pathogen with intraspecies classification ability. Graphical abstract MALDI-MS classified Foc isolates by aggressiveness level on banana revealing the additional influence of location and host cultivar on the expression of lipids and proteins.