Accelerated ageing test and enzymatic expression in the evaluation of kale seed quality / Teste de envelhecimento acelerado e expressão enzimática na avaliação da qualidade de sementes de couve

The demand for high-quality vegetable seeds and the production of vigorous seedlings has increased in recent years, as these characteristics are determining factors of production success. Vegetables are growing in national importance, and kale stands out as an important source of income for small farmers. The objective of this study was to adapt the traditional accelerated aging test methodology with a saturated NaCl solution of kale seeds and evaluate the enzymatic activity after the vigour test. Six batches of kale seeds were used, and the moisture content, weight of one thousand seeds, first germination count, germination, germination speed index, emergence, initial stand, and emergence velocity index were determined. In the accelerated ageing test, the seeds were submitted to the traditional accelerated ageing test method and the accelerated ageing test method with a saturated NaCl solution for ageing periods of 0, 24, 48, 72, and 96 hours. Electrophoretic analysis of superoxide dismutase (SOD), catalase (CAT), and alcohol dehydrogenase (ADH) isoenzymes was also performed. The accelerated ageing test, using the traditional method for 72 hours at 41 °C, is adequate for evaluating the physiological potential of kale seeds. The isoenzymatic analyses of SOD, CAT, and ADH demonstrate that the biochemical markers are efficient at differentiating kale seeds after accelerated ageing.(AU)

A demanda por sementes de hortaliças com alta qualidade e a obtenção de mudas vigorosas tem aumentado nos últimos anos, pois estas características constituem fatores determinantes do êxito da produção. As hortaliças têm uma importância nacional crescente, dentre elas se destaca a couve, por ser importante fonte de renda para pequenos agricultores. Objetivou-se adequar a metodologia do teste de envelhecimento acelerado tradicional e com solução saturada de NaCl em sementes de couve e avaliar a atividade enzimática após o teste de vigor. Foram utilizados seis lotes de sementes de couve e determinouse o grau de umidade, peso de mil sementes, primeira contagem de germinação, germinação, índice de velocidade de germinação, emergência, estande inicial e índice de velocidade de emergência. No teste de envelhecimento acelerado, as sementes foram submetidas ao método tradicional e com solução saturada de NaCl, por períodos de 0; 24; 48; 72 e 96 horas. Realizou-se também a análise eletroforética das isoenzimas superóxido dismutase (SOD), catalase (CAT) e álcool desidrogenase (ADH). O teste de envelhecimento acelerado, utilizando o método tradicional na combinação de 72 horas a 41 °C é adequado para avaliar o potencial fisiológico de sementes de couve. As análises isoenzimáticas superóxido dismutase (SOD), catalase (CAT) e álcool desidrogenase (ADH) demonstram que os marcadores bioquímicos são eficientes e promissores na diferenciação de sementes de couve após o envelhecimento acelerado.(AU)

Uso de ramas de batata-doce para produção de feno / The use of the sweet potato branches in the hay production

Ramas de batata-doce apresentam características satisfatórias para alimentação animal, porém não têm sido exploradas na forma de feno. Objetivou-se avaliar o uso de ramas de batata-doce para a produção de feno e a divergência genética em clones de batata-doce. Foram avaliados dez clones de batata-doce, conduzidos a campo em blocos ao acaso e cinco repetições. Foram determinados o teor de matéria seca, as produtividades de massa verde e massa seca (PMS), a relação folha e haste das ramas e a produção total e comercial de raízes (PRC). Obtidos os fenos, avaliaram-se as características MS, PB, FDN, FDA, lignina, amido, açúcares solúveis, macro e micronutrientes. Estimou-se a divergência genética entre os clones de batata-doce. O clone UFVJM-54 aliou alta PRC com maior PMS. Os fenos apresentaram teores de PB variando de 11,99 a 15,09%, FDN 47,22 a 55,55%, FDA 22,92 a 28%, e 6,87 a 13,72% de LIG. De forma geral, os teores de minerais nos fenos foram satisfatórios. Os clones foram separados em cinco grupos diferentes. As ramas de batata-doce podem ser utilizadas para a produção de feno. Os clones UFVJM-37, UFVJM-46 e UFVJM-54 são recomendados para cruzamentos com os demais genótipos.(AU)

Sweet potato branches have satisfactory characteristics for animal feed, but have not been exploited in the form of hay. The objective of this work was to evaluate the use of the sweet potato branches for the hay production and genetic divergence in sweet potato clones. Were evaluated ten sweet potato clones, conducted on the field in randomized blocks and 5 repetitions. Dry matter content, green mass and dry mass yield (DMY), leaf and stem ratio of branches and total and commercial root production (CRP) were determined. Gotten the hays, were evaluated the characteristics: DM, CP, NDF, ADF, lignin, starch, soluble sugars, macro and micronutrients. The genetic divergence among the sweet potato clones was estimated. The UFVJM-54 clone allied high CRP with bigger DMY. The hay presented content of CP ranging from 11.99% to 15.09%, NFD 47.22 to 55.55%, ADF 22.92 to 28%, and 6.87 to 13.72% of LIG. In a general way, the contents of minerals in hays were satisfactory. The clones were separated into 5 different groups. The sweet potato branches can be used for the hay production. The UFVJM-37, UFVJM-46 and UFVJM-54 clones are recommended for crossing with the other genotypes.(AU)

Uso de ramas de batata-doce para produção de feno / The use of the sweet potato branches in the hay production

Ramas de batata-doce apresentam características satisfatórias para alimentação animal, porém não têm sido exploradas na forma de feno. Objetivou-se avaliar o uso de ramas de batata-doce para a produção de feno e a divergência genética em clones de batata-doce. Foram avaliados dez clones de batata-doce, conduzidos a campo em blocos ao acaso e cinco repetições. Foram determinados o teor de matéria seca, as produtividades de massa verde e massa seca (PMS), a relação folha e haste das ramas e a produção total e comercial de raízes (PRC). Obtidos os fenos, avaliaram-se as características MS, PB, FDN, FDA, lignina, amido, açúcares solúveis, macro e micronutrientes. Estimou-se a divergência genética entre os clones de batata-doce. O clone UFVJM-54 aliou alta PRC com maior PMS. Os fenos apresentaram teores de PB variando de 11,99 a 15,09%, FDN 47,22 a 55,55%, FDA 22,92 a 28%, e 6,87 a 13,72% de LIG. De forma geral, os teores de minerais nos fenos foram satisfatórios. Os clones foram separados em cinco grupos diferentes. As ramas de batata-doce podem ser utilizadas para a produção de feno. Os clones UFVJM-37, UFVJM-46 e UFVJM-54 são recomendados para cruzamentos com os demais genótipos.

Sweet potato branches have satisfactory characteristics for animal feed, but have not been exploited in the form of hay. The objective of this work was to evaluate the use of the sweet potato branches for the hay production and genetic divergence in sweet potato clones. Were evaluated ten sweet potato clones, conducted on the field in randomized blocks and 5 repetitions. Dry matter content, green mass and dry mass yield (DMY), leaf and stem ratio of branches and total and commercial root production (CRP) were determined. Gotten the hays, were evaluated the characteristics: DM, CP, NDF, ADF, lignin, starch, soluble sugars, macro and micronutrients. The genetic divergence among the sweet potato clones was estimated. The UFVJM-54 clone allied high CRP with bigger DMY. The hay presented content of CP ranging from 11.99% to 15.09%, NFD 47.22 to 55.55%, ADF 22.92 to 28%, and 6.87 to 13.72% of LIG. In a general way, the contents of minerals in hays were satisfactory. The clones were separated into 5 different groups. The sweet potato branches can be used for the hay production. The UFVJM-37, UFVJM-46 and UFVJM-54 clones are recommended for crossing with the other genotypes.

Visual symptoms of nutrient deficiencies in Physalis peruviana L / Sintomas visuais de deficiências nutricionais em fisalis, Physalis peruviana L

The physalis production has caused interest of producers, consumers and traders due to its easy growing, high nutritional value and economic value added. The objective of this study was to evaluate the growth and characterize the symptoms of macro and micronutrient deficiencies in physalis seedlings (Physalis peruviana L.). The seedlings were grown in complete nutrient solution and also in solutions with individual omissions of N, P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn, using the missing element technique. Visual symptoms of nutrient deficiency and the dry matter production of shoot and root, respectively taken at 68 and 150 days after treatment application were evaluated. Omissions of macro and micronutrients caused visual symptoms of nutrient deficiencies common to other species. Nutrient deficiencies limited the total dry matter production in the following order: N > S> P> K > Ca > Mg for macronutrients and Fe > B > Zn > Mn > Cu for micronutrients, respectively.

A produção de fisalis tem despertado interesse de produtores, consumidores e comercializadores devido seu fácil cultivo, grande valor nutricional e econômico agregado. O objetivo deste trabalho foi avaliar o crescimento e caracterizar os sintomas de deficiências de macro e micronutrientes em mudas de fisalis (Physalis peruviana L.). As mudas foram cultivadas em solução nutritiva completa e, também em soluções com omissões individuais de N, P, K, Ca, Mg, S, B, Cu, Fe, Mn e Zn, pelo uso da técnica do elemento faltante. Foram avaliados os sintomas visuais de deficiência de nutrientes, e a produção de matéria seca da parte aérea e das raízes aos 68 e 150 dias após a aplicação dos tratamentos, respectivamente. As omissões de macro e micronutrientes provocaram sintomas visuais de deficiências nutricionais comuns a outras espécies. As deficiências limitaram a produção de matéria seca total na seguinte ordem: N > S > P > K > Ca > Mg para macronutrientes e Fe > B > Zn > Mn > Cu, para micronutrientes, respectivamente.

Longitudinal data assessment of global stability index in kale leaves

Kale plants are usually sold in natura in street markets and malls. Kale leaves can have their appearance compromised by dehydration and discoloration due to increased post-harvest time exposure. We aimed to analyze the Global Stability Index (GSI) in kale accessions by means of repeated measurement analysis and curve grouping as a complementary form of superior sample identification with regard to post-harvest preservation. Thirty kale accessions were evaluated using a randomized block design with four blocks and five plants per plot. Two commercial leaves per plant were collected, and kept on workbenches in the shade at a temperature of 18 ± 1 °C. Subsequently, the degrees of discoloration and dehydration, total chlorophyll content, and accumulated fresh mass loss were evaluated over a 15-day period. From these data, the GSI was calculated for each day of evaluation. In addition, using mixed models, thirteen co-variance structures were tested. For graphical analysis, thirteen linear and non-linear models were assessed followed by curve grouping using multivariate analysis. The GSI was efficient for differentiating accessions, which became an important tool in post-harvest studies. GSI values were not equally correlated, therefore the use of mixed models became an important approach. The unstructured matrix was the best fit to model the dependence of error. The Melow I model was the best fit for studying the GSI. The accessions UFVJM-10, UFLA-1, COM-1, UFVJM-32, COM-3, UFVJM-8, UFVJM-36 and UFVJM-24, belonging to 3 and 5 clusters, are recommended for crop cultivation and as parental material in breeding programs.

Longitudinal data assessment of global stability index in kale leaves

Kale plants are usually sold in natura in street markets and malls. Kale leaves can have their appearance compromised by dehydration and discoloration due to increased post-harvest time exposure. We aimed to analyze the Global Stability Index (GSI) in kale accessions by means of repeated measurement analysis and curve grouping as a complementary form of superior sample identification with regard to post-harvest preservation. Thirty kale accessions were evaluated using a randomized block design with four blocks and five plants per plot. Two commercial leaves per plant were collected, and kept on workbenches in the shade at a temperature of 18 ± 1 °C. Subsequently, the degrees of discoloration and dehydration, total chlorophyll content, and accumulated fresh mass loss were evaluated over a 15-day period. From these data, the GSI was calculated for each day of evaluation. In addition, using mixed models, thirteen co-variance structures were tested. For graphical analysis, thirteen linear and non-linear models were assessed followed by curve grouping using multivariate analysis. The GSI was efficient for differentiating accessions, which became an important tool in post-harvest studies. GSI values were not equally correlated, therefore the use of mixed models became an important approach. The unstructured matrix was the best fit to model the dependence of error. The Melow I model was the best fit for studying the GSI. The accessions UFVJM-10, UFLA-1, COM-1, UFVJM-32, COM-3, UFVJM-8, UFVJM-36 and UFVJM-24, belonging to 3 and 5 clusters, are recommended for crop cultivation and as parental material in breeding programs.(AU)

Performance of carrot genotypes at two Jequitinhonha Valley sites / Desempenho de cultivares de cenoura em duas regiões distintas do Alto Vale do Jequitinhonha, MG

The successful commercial use of carrot depends on the choice of cultivars that are well-adapted to soil and climate conditions at the cultivation site and on good consumer acceptance. The objective of this study was to evaluate the performance of carrot cultivars grown in autumn-winter in two towns with different climatic characteristics, in the High Jequitinhonha Valley, MG, Brazil. The experiments were conducted on the Rio Manso farm, in the town of Couto de Magalhães de Minas and on Campus JK at UFVJM, in the town of Diamantina, Minas Gerais, Brazil. Six carrot cultivars (Brasília, Nantes, Kuronan, Esplanada, Planalto and Tornado) were evaluated using a randomized complete block design with three replicates. The following morpho-agronomic characteristics were evaluated 100 days after sowing: shoot height, root length, root dry matter, total dry matter, harvest index, commercial yield and total yield root. The most suitable cultivars in Diamantina were Planalto, Tornado and Kuronan, and the cultivatar Planalto was the best suited to Couto de Magalhães de Minas. The agronomic performance of the cultivars was higher in Couto de Magalhães de Minas for the majority of the study variables, resulting in a higher total yield and commercial root yield. The climatic and soil conditions of Diamantina induced flowering in most cultivars, causing a loss to the commercial yield in autumn-winter cultivation.

O sucesso na exploração comercial da cenoura depende da escolha de cultivares com boa adaptação às condições de solo e de clima no local onde será cultivada e da boa aceitação pelo mercado consumidor. O objetivo do presente trabalho foi avaliar o desempenho de cultivares de cenoura no cultivo de outono-inverno em dois municípios com características edafoclimáticas distintas do Alto Vale do Jequitinhonha, MG. Os experimentos foram conduzidos na Fazenda Rio Manso, município de Couto de Magalhães de Minas, MG e no Campus JK da UFVJM, município de Diamantina, MG. Foram avaliadas seis cultivares de cenoura (Brasília, Nantes, Kuronan, Esplanada, Planalto e Tornado) utilizando-se o delineamento experimental em blocos ao acaso com três repetições. As características morfoagronomicas altura da parte aérea, comprimento de raiz, matéria seca de raiz, matéria seca total, índice de colheita, produtividade comercial e produtividade total de raízes foram avaliadas 100 dias após a semeadura. As cultivares que se destacaram em Diamantina foram Planalto, Tornado e Kuronan e em Couto de Magalhães de Minas a cultivar Planalto. O desempenho agronômico das cultivares foi superior em Couto de Magalhães de Minas para a maioria das variáveis avaliadas, resultando em uma maior produtividade total e comercial de raízes. As condições edafoclimáticas de Diamantina induzem florescimento na maioria das cultivares, causando perdas de produtividade comercial no cultivo deoutono-inverno.

Performance of carrot genotypes at two Jequitinhonha Valley sites / Desempenho de cultivares de cenoura em duas regiões distintas do Alto Vale do Jequitinhonha, MG

The successful commercial use of carrot depends on the choice of cultivars that are well-adapted to soil and climate conditions at the cultivation site and on good consumer acceptance. The objective of this study was to evaluate the performance of carrot cultivars grown in autumn-winter in two towns with different climatic characteristics, in the High Jequitinhonha Valley, MG, Brazil. The experiments were conducted on the Rio Manso farm, in the town of Couto de Magalhães de Minas and on Campus JK at UFVJM, in the town of Diamantina, Minas Gerais, Brazil. Six carrot cultivars (Brasília, Nantes, Kuronan, Esplanada, Planalto and Tornado) were evaluated using a randomized complete block design with three replicates. The following morpho-agronomic characteristics were evaluated 100 days after sowing: shoot height, root length, root dry matter, total dry matter, harvest index, commercial yield and total yield root. The most suitable cultivars in Diamantina were Planalto, Tornado and Kuronan, and the cultivatar Planalto was the best suited to Couto de Magalhães de Minas. The agronomic performance of the cultivars was higher in Couto de Magalhães de Minas for the majority of the study variables, resulting in a higher total yield and commercial root yield. The climatic and soil conditions of Diamantina induced flowering in most cultivars, causing a loss to the commercial yield in autumn-winter cultivation.(AU)

O sucesso na exploração comercial da cenoura depende da escolha de cultivares com boa adaptação às condições de solo e de clima no local onde será cultivada e da boa aceitação pelo mercado consumidor. O objetivo do presente trabalho foi avaliar o desempenho de cultivares de cenoura no cultivo de outono-inverno em dois municípios com características edafoclimáticas distintas do Alto Vale do Jequitinhonha, MG. Os experimentos foram conduzidos na Fazenda Rio Manso, município de Couto de Magalhães de Minas, MG e no Campus JK da UFVJM, município de Diamantina, MG. Foram avaliadas seis cultivares de cenoura (Brasília, Nantes, Kuronan, Esplanada, Planalto e Tornado) utilizando-se o delineamento experimental em blocos ao acaso com três repetições. As características morfoagronomicas altura da parte aérea, comprimento de raiz, matéria seca de raiz, matéria seca total, índice de colheita, produtividade comercial e produtividade total de raízes foram avaliadas 100 dias após a semeadura. As cultivares que se destacaram em Diamantina foram Planalto, Tornado e Kuronan e em Couto de Magalhães de Minas a cultivar Planalto. O desempenho agronômico das cultivares foi superior em Couto de Magalhães de Minas para a maioria das variáveis avaliadas, resultando em uma maior produtividade total e comercial de raízes. As condições edafoclimáticas de Diamantina induzem florescimento na maioria das cultivares, causando perdas de produtividade comercial no cultivo deoutono-inverno.(AU)

Potencial de silagens de ramas de batata-doce para alimentação animal / Potential of silages of sweet-potato foliages for animal feeding

Objetivou-se avaliar o potencial de silagens de ramas de diferentes clones de batata-doce para alimentação animal. O trabalho foi realizado na Fazenda Forquilha localizada no Distrito de Batatal, município de Diamantina MG, no período de 23/12/2007 a 23/06/2008. O experimento foi conduzido no esquema de parcelas subdivididas em delineamento de blocos completos casualizados. Foram avaliadas a produtividade de matéria verde e matéria seca das ramas e a composição químico-bromatológica, além do perfil fermentativo de suas silagens, em três idades de colheita (120, 150 e 180 dias), em oito clones de batata doce. A produtividade de matéria seca não variou com a época de colheita das ramas, obtendo-se média de 6,01t ha-1. Os teores de matéria seca nas ramas aumentaram com o ciclo da cultura, verificando-se teores médios de 11,94; 12,16 e 19,62 por cento, aos 120, 150 e 180 dias após o corte, respectivamente. As silagens das ramas de batata-doce apresentaram altos teores protéicos e energéticos e adequado perfil fermentativo, portanto, apresentam potencial para utilização na alimentação animal, independentemente dos clones.

The objective was to evaluate the potential of silage derived from different clones of sweet potato for animal feed. The study was conducted at Forquilha Farm located in Batatal district, MG Diamantina in the period from 23/12/2007 to 23/06/2008. The experiment was conducted in split plot design in a randomized complete block. It was evaluated the green and dry matter yield, the chemical composition and the fermentative profile of silage harvested on three dates (days 120, 150 and 180), of eight clones of sweet potato. The dry matter yield did not vary according to the harvest time of the branches, resulting in an average of 6.01t ha-1. The dry matter content in the stems increased with the growth cycle, and there are average levels of 11.94, 12.16 and 19.62 percent on days 120, 150 and 180 after cutting, respectively. The raw potato silage showed high protein content and adequate energy fermentation and therefore, potential for use in animal feed, regardless of clones.

Época de maturação, dispersão, colheita e qualidade fisiológica de sementes de sempre-viva (Syngonanthus elegans (Bong. ) Ruhland) / Maturation time, dispersion, crop and physiological quality of star flowers (Syngonanthus elegans (Bong. ) Ruhland) seeds

Neste trabalho, objetivou-se avaliar a época de maturação, dispersão, colheita e a qualidade fisiológica de sementes de sempreviva (Syngonanthus elegans (Bong.) Ruhland), em Diamantina, MG. Capítulos de S. elegans foram colhidos em cinco épocas (20/06/ 05, 20/07/05, 20/08/05, 20/09/05 e 20/10/05) em três locais de produção natural, dentro do Campus da UFVJM em um Neossolo Quartzarênico Órtico Típico (locais 1 e 2) e Neossolo Quartzarênico Hidromórfico Típico (local 3) e, posteriormente, submetidos a testes para avaliação do vigor e germinação das sementes. A qualidade fisiológica de sementes de S. elegans variou em função da época e local de colheita. Estratégias de manejo devem ser realizadas como a colheita após o início do período de dispersão das sementes (junho a outubro) e com os capítulos totalmente abertos.

The aims of this work were to evaluate the maturation time, dispersion, crop and the physiological quality seeds of star flowers (Syngonanthus elegans (Bong.) Ruhland), in Diamantina, MG. S. elegans Chapters were picked up in five times (20/06/05, 20/07/05, 20/ 08/05, 20/09/05 and 20/10/05) in three places of natural production, inside the Campus of UFVJM in a Neossolo Quartzarênico Órtico Típico (places 1 e 2) and Neossolo Quartzarênico Hidromórfico Típico (place 3) and after submitted to the germination and vigor tests. Physiological quality of seeds of S. elegans were affected by time and crop place. Crop management should be accomplished as the harvest after the beginning of the period of dispersion of seeds (june to october) and with the chapters totally open.