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1.
Phytochemistry ; 134: 106-113, 2017 Feb.
Article in English | MEDLINE | ID: mdl-27889243

ABSTRACT

Five alkaloids, in addition to hydroxytyrosol and uridine, were isolated from aerial parts of Alternanthera littoralis P. Beauv. Among the isolated compounds, alternamide A was an unusual tricyclic alkaloid with a bridged benzoazepine core. All isolated alkaloids have a catechol moiety, indicating a possible common biosynthetic route. Their structures were established by 1D and 2D NMR spectroscopy in combination with extensive tandem MS experiments by collisional induced dissociation (CID). The antiprotozoal activity of the isolated compounds was assayed against trypomastigote forms of Trypanosoma cruzi and amastigotes of Leishmania amazonensis. Alternamine A was the most active compound, reducing markedly the viability of both parasites. Antioxidant capacities evaluated by ORACFL assay showed that the isolated alkaloids (mainly alternamide B) contributed to the high activity recorded for the ethanolic crude extract; possibly, the catechol moiety present in all structures plays a central role in this result.


Subject(s)
Alkaloids/isolation & purification , Alkaloids/pharmacology , Amaranthaceae/chemistry , Antioxidants/isolation & purification , Antioxidants/pharmacology , Antiprotozoal Agents/isolation & purification , Antiprotozoal Agents/pharmacology , Alkaloids/chemistry , Antioxidants/chemistry , Antiprotozoal Agents/chemistry , Brazil , Catechols/chemistry , Leishmania/drug effects , Nuclear Magnetic Resonance, Biomolecular , Trypanosoma cruzi/drug effects
2.
Curr Drug Targets ; 14(9): 1015-22, 2013 Aug.
Article in English | MEDLINE | ID: mdl-23547779

ABSTRACT

This study was undertaken to evaluate the effect of Alternathera brasiliana (Amaranthaceae) extracts as photosensitizing agents in photodynamic antimicrobial therapies (PACT) against Staphylococcus aureus, Staphylococcus epidermidis and Candida dubliniensis. The crude hexane and ethanol extracts were obtained from A. brasiliana whole plant and showed absortion from 650 to 700 nm. Also, singlet molecular oxygen (1O2) production (type II photosensitization reaction) was examined, and the results show that 1,3-diphenylisobenzofuran photodegradation was greatly enhanced in the presence of the A. brasiliana extracts. One plate in each assay was irradiated while the other was not irradiated, the number of colony-forming units per milliliter (CFU/mL) was obtained, and data analyzed by the Tukey test. The chemical composition of the extracts was determined by chromatographic and spectrometric techniques; steroids, triterpenes, and flavonoids were identified. Laser irradiation alone at 685 nm using diode laser, output power of 35 mW, and energy of 28 J/cm2, or non-irradiated crude extracts in sub-inhibitory concentration did not reduce the number of CFU/mL significantly, whereas irradiated hexane and ethanol extracts, in sub-inhibitory concentrations, inhibited the growth of these microorganisms. The photoactivation of hexane and ethanol extracts of A. brasiliana, in sub-inhibitory concentrations, using red laser radiation at 685 nm had an antimicrobial effect.


Subject(s)
Amaranthaceae/chemistry , Anti-Bacterial Agents/pharmacology , Antifungal Agents/pharmacology , Bacteria/drug effects , Fungi/drug effects , Photochemotherapy , Photosensitizing Agents/pharmacology , Plant Extracts/pharmacology , Anti-Bacterial Agents/chemistry , Antifungal Agents/chemistry , Candida/drug effects , Ethanol , Flavonoids/analysis , Hexanes , Photosensitizing Agents/chemistry , Plant Extracts/chemistry , Singlet Oxygen/metabolism , Staphylococcus/drug effects , Steroids/analysis , Triterpenes/analysis
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