ABSTRACT
The effects of complex extract from Aconitum baikalense on reparative regeneration of a plane dorsal skin wound were studied. Treatment with Aconitum baikalense tincture stimulated reparation and skin regeneration. The effects of the Aconitum baikalense alkaloids on functional activity of fibroblast precursors were studied in vitro by cultural methods. Mesaconitine, hypaconitine, songorine, napelline, and 12-epinapelline N-oxide significantly stimulated the growth of colonies from fibroblast precursors. This indicated direct stimulation of fibroblasts by aconite alkaloids, which could be a mechanism of reparative activity of the complex extract.
Subject(s)
Aconitum/chemistry , Plant Extracts/pharmacology , Regeneration/drug effects , Skin/drug effects , Wound Healing/drug effects , Aconitine/analogs & derivatives , Aconitine/isolation & purification , Aconitine/pharmacology , Alkaloids/isolation & purification , Alkaloids/pharmacology , Animals , Cell Proliferation/drug effects , Cells, Cultured , Colony-Forming Units Assay , Female , Fibroblasts/cytology , Fibroblasts/drug effects , Mice , Mice, Inbred CBA , Plant Components, Aerial/chemistry , Plant Extracts/chemistry , Regeneration/physiology , Wound Healing/physiologyABSTRACT
Experimental investigations have shown that water-alcohol extracts from plants containing alkaloids (Aconitum baikalense, Aconitum septentrionale, Delphinium elatum L., Conium maculatum) and salicylic acid (Filipendula ulmaria, Salix viminalis, Fragaria vesca, Rubus idaeus) inhibited the development of main symptoms of inflammation, viz. exudation, pain, fever, to the same extent as non-steroidal anti-inflammatory agents. The substances studied in this work may be used to develop new efficient pharmacological preparations for the treatment of different inflammatory conditions associated with severe pain syndrome.
Subject(s)
Inflammation/drug therapy , Phytotherapy/methods , Plant Extracts/therapeutic use , Aconitum/chemistry , Alkaloids/analysis , Animals , Conium/chemistry , Delphinium/chemistry , Disease Models, Animal , Female , Fragaria/chemistry , Male , Mice , Plant Extracts/chemistry , Rats , Salicylic Acid/analysis , Salix/chemistry , Siberia , Treatment OutcomeABSTRACT
Antiinflammatory effects of water-alcohol extracts and sum of alkaloids from the aerial part of delphinium under conditions of immune inflammation caused by Freund's complete adjuvant were comparable to activity of orthophen, a classical antiinflammatory drug. Normalizing effects of the studied extracts on hematological and biochemical parameters were demonstrated.
Subject(s)
Alkaloids/therapeutic use , Anti-Inflammatory Agents/therapeutic use , Arthritis, Experimental/drug therapy , Delphinium/chemistry , Freund's Adjuvant/pharmacology , Plant Extracts/therapeutic use , Alkaloids/chemistry , Animals , Anti-Inflammatory Agents/chemistry , Arthritis, Experimental/chemically induced , Arthritis, Experimental/pathology , Edema/chemically induced , Edema/drug therapy , Extremities , Hyperemia/chemically induced , Hyperemia/drug therapy , Male , Plant Extracts/chemistry , RatsABSTRACT
It is demonstrated in experiment that the entire complex and individual agents isolated from the above-ground part of bee larkspur Delphinium elatum stimulate the reparative regeneration of skin in white mongrel mice. The maximum activity was exhibited by the alkaloid fraction of Delphinium elatum.
Subject(s)
Delphinium/chemistry , Plant Extracts/pharmacology , Skin/drug effects , Wound Healing/drug effects , Animals , Female , Mice , Phytotherapy , Skin/injuries , Skin/pathology , Wound Healing/physiologyABSTRACT
Effects of complex and individual bioactive substances extracted from tall delphinium on acute inflammation induced by carrageenin, acetic acid, serotonin, and histamine were studied. The studied plant substances produced a pronounced antiinflammatory effect comparable to that of nonsteroid antiinflammatory drugs.
