ABSTRACT
The natural distribution range of Aedes koreicus is Korea, China, Japan, and the Russian Far East. Since 2008, this species has been recorded as an invasive species in some European countries (Belgium, European Russia, Germany, Hungary, Italy, Slovenia, and Switzerland). The invasive mosquito species Ae. koreicus is reported from the Republic of Kazakhstan for the first time. Its morphological identification was confirmed by molecular-genetic analyses of ND4 sequences using specific primers. Aedes koreicus larvae were found in an artificial water reservoir together with the larvae of Culiseta longiareolata and Culex pipiens s.l. Aedes koreicus successfully overwintered in Almaty at low winter temperatures in 2018-2019. This suggests that the Ae. koreicus acclimation capacity is greater than it has been considered until now. We assume that Ae. koreicus will spread over the west and south of the Republic of Kazakhstan and territories of Kyrgyzstan and Uzbekistan Republics bordering the Almaty region.
TITLE: Premier signalement de l'espèce de moustique envahissante Aedes koreicus (Diptera, Culicidae) en République du Kazakhstan. ABSTRACT: L'aire de répartition naturelle d'Aedes koreicus est la Corée, la Chine, le Japon et l'Extrême-Orient russe. Depuis 2008, cette espèce a été signalée comme espèce envahissante dans certains pays européens (Belgique, Russie européenne, Allemagne, Hongrie, Italie, Slovénie et Suisse). L'espèce de moustique envahissante Ae. koreicus est signalée ici pour la première fois en République du Kazakhstan. Son identification morphologique a été confirmée par des analyses de génétique moléculaire de la séquence ND4 à l'aide d'amorces spécifiques. Des larves d'Aedes koreicus ont été trouvées dans un réservoir d'eau artificiel avec des larves de Culiseta longiareolata et Culex pipiens s.l. Aedes koreicus a hiverné avec succès à Almaty à de basses températures hivernales en 2018-2019. Cela suggère que la capacité d'acclimatation d'Ae. koreicus est supérieure à ce qui avait été envisagé jusqu'à présent. Nous supposons qu'Ae. koreicus s'étendra sur l'ouest et le sud de la République du Kazakhstan et les territoires du Kirghizistan et des républiques d'Ouzbékistan bordant la région d'Almaty.
Subject(s)
Aedes , Culicidae , Aedes/genetics , Animals , China , Europe , Germany , Hungary , Introduced Species , Italy , Japan , Kazakhstan , Republic of Korea , Russia , SloveniaABSTRACT
In this study, cytogenetic analysis of the metaphase chromosomes from imaginal discs of Aedes (Diptera: Culicidae) mosquitoes-Aedes communis, Ae. punctor, Ae. intrudens, and Ae. rossicus-was performed. The patterns of C-banding and DAPI staining of the heteroÑhromatin and the length of the chromosomes demonstrate species specificity. In particular, the Ae. punctor chromosomes are the shortest compared with Ae. communis, Ae. intrudens, and Ae. rossicus, and they also carry additional C and DAPI bands in intercalary regions. The Ae. intrudens chromosomes are the longest, they have pericentromeric C bands, and they almost lack any DAPI bands near the centromere of chromosome 3 versus Ae. communis, which has the largest pericentromeric DAPI blocks in all three chromosome pairs. Ae. rossicus also possesses DAPI bands in the centromeric regions of all chromosomes, but their staining is weaker compared with those of Ae. communis. Therefore, the analysis of karyotypes is a tool for species-level identification of these mosquitoes.
ABSTRACT
Karyotypes of Aedes (Culicidae) mosquitoes (Ae. excrucians, Ae. behningi, and Ae. euedes) have been analyzed using the metaphase chromosomes of imaginal discs. Lacto-aceto-orcein, C-banding, and DAPI staining have detected species-specific features in the morphology and lengths of these chromosomes in the examined species. Species-specific features of chromosome 1 in the location of heterochromatin blocks have been shown. Thus, the metaphase chromosomes in the imaginal discs of Ae. excrucians, Ae. behningi, and Ae. euedes are a characteristic for species identification of mosquito species.
Subject(s)
Aedes/genetics , Chromosomes, Insect/genetics , Malaria/transmission , Mosquito Vectors/genetics , Plasmodium/physiology , Aedes/parasitology , Aedes/physiology , Animals , Karyotype , Karyotyping , Metaphase , Mosquito Vectors/parasitology , Mosquito Vectors/physiology , Species SpecificityABSTRACT
Herein, we report the results of study of Anopheles species in Primorsk and Khabarovsk regions of Russia. Three species of the Anopheles hyrcanus group: An. kleini, An. pullus, and An. lesteri were identified by molecular taxonomic diagnostics for the first time in Russia. Surprisingly, An. sinensis, which earlier was considered the only species of Anopheles in Russian Far East, was not observed. We analyzed nucleotide variation in the 610-bp fragment of the 5' end of the cytochrome c oxidase subunit I (COI) region. All species possessed a distinctive set of COI sequences. A maximum likelihood phylogenetic tree was constructed for members of the hyrcanus group. The examined Anopheles hyrcanus group members could be divided into two major subgroups: subgroup 1 (An. hyrcanus and An. pullus) and subgroup 2 (An. sinensis, An. kleini, and An. lesteri), which were found to be monophyletic.
