ABSTRACT
The paper presents data on the comparative analysis of nucleotide sequences of a S3 gene fragment of 67 chicken reovirus (CRV) isolates from the abnormal biopsy specimens tested in 1999 to 2007. These CRV isolates were ascertained to differ from vaccine strains in the nucleotide sequence of the S3 gene. The approximate substitution rates for the S3 gene were established to range from 2.0 x 10(-3) to 6.0 x 10(-3) nucleotide substitutions per year.
Subject(s)
Capsid Proteins/genetics , Chickens/genetics , Poultry Diseases/virology , RNA-Binding Proteins/genetics , Reoviridae Infections/veterinary , Reoviridae/genetics , Animals , Base Sequence , Capsid Proteins/classification , Molecular Sequence Data , Phylogeny , RNA-Binding Proteins/classification , Reoviridae/isolation & purification , Reoviridae Infections/virology , Russia , Sequence Analysis, DNA , Sequence Homology, Nucleic AcidABSTRACT
The method of chicken reovirus strain differentiation was worked out on the basis of RT-PCR and heteroduplex mobility assay (HMA). The S3 gene cDNA (633-896 b.p.) of some Russian and Italian chicken reovirus isolates was amplified by RT-PCR. The analysis of these cDNA samples was carried out by HMA. The relation between nucleotide differences and relative mobility of compared cDNA heteroduplex was reflected by the regression curve. The equation of linear regression was derived (y = 91.726-0.89x; where y is the level of nucleotide difference of compared cDNA (%), x is the relative mobility of compared cDNA heteroduplex (%)). This method made it possible to take correct results within 5-35% of nucleotide difference in heteroduplex sequences.
