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1.
J Vet Cardiol ; 35: 1-7, 2021 Jun.
Article in English | MEDLINE | ID: mdl-33789180

ABSTRACT

A 9-month-old French Bulldog with pulmonary stenosis (PS) underwent transthoracic echocardiography (TTE) and electrocardiogram-gated coronary computed tomography angiography (ECG-CCTA) for presurgical planning of pulmonary balloon valvuloplasty (PBV). Transthoracic echocardiography revealed severe PS and identified two distinct coronary ostia (left and right). Electrocardiogram-gated coronary computed tomography angiography showed a circumpulmonary course of the interventricular paraconal coronary artery, which abnormally originated from the right coronary artery. Based on this case report, the echocardiographic identification of two coronary ostia does not rule out a coronary artery anomaly with circumpulmonary course (as previously hypothesized), and coronary computed tomography angiography may be recommended for presurgical planning of PBV in French Bulldogs.


Subject(s)
Coronary Artery Disease , Dog Diseases , Pulmonary Valve Stenosis , Animals , Computed Tomography Angiography/veterinary , Coronary Angiography , Coronary Artery Disease/complications , Coronary Artery Disease/veterinary , Dog Diseases/diagnostic imaging , Dog Diseases/surgery , Dogs , Echocardiography/veterinary , Pulmonary Valve Stenosis/complications , Pulmonary Valve Stenosis/diagnostic imaging , Pulmonary Valve Stenosis/veterinary
2.
J Biol Regul Homeost Agents ; 34(4 Suppl. 3): 197-202. Congress of the Italian Orthopaedic Research Society, 2020.
Article in English | MEDLINE | ID: mdl-33261277

ABSTRACT

Nowadays, despite the possibility to use in vitro or computer models in research, animal models are still essential. Different animal models are available for meniscal repair investigation. Although a unique perfect model for the structure of the human's knee does not exist, the choice of the proper animal model is crucial for a correct research. The principal animal models in the meniscal repair are sheep, goats, pigs and dogs. Each of these has pros and cons for their utilization. Analysing each pro and con is essential for optimizing the choice of the animal model, which depends on the experimental question, avoiding unnecessary waste of resources and minimizing the animal suffering, according to the Russell and Burch's three "Rs" principles (Reduce, Refine and Recycle). In this concise review, we resume the meniscus anatomical features of the main large animals, to help choose the most suitable animal model for subsequent studies on meniscal repair.


Subject(s)
Meniscus , Tibial Meniscus Injuries , Animals , Knee Joint , Menisci, Tibial
3.
Forensic Sci Int ; 297: 8-15, 2019 Apr.
Article in English | MEDLINE | ID: mdl-30743064

ABSTRACT

Age estimation in growing dogs is crucial not only in clinical practice but increasingly so in forensic practice as well. In the last few years, it has assumed great importance for correctly identifying the age of puppies illegally imported to Italy as well as to other European countries. Puppies are, in fact, transported when they are too young to be moved, which can cause both animal/public health and animal welfare issues. Therefore, the movement of animals within the European Community is governed by strict rules, and veterinarians are often required to evaluate the age of the imported puppies in a forensic scenario as accurately as possible. To date, X-ray evaluation of limb bones ossification centers (OCs) is generally accepted as a valid tool to assess the age of puppies. A wealth of information exists on this topic but it is not always easily available. This work is a historical review of the existing literature and proposes two tables illustrating the timelines of limb OCs appearance and closure, coming to terms with forensic requests to evaluate the age of a puppy. The timelines reported indicate the need to improve methodology to enhance the accuracy and to reduce the error in age estimation.


Subject(s)
Age Determination by Skeleton/methods , Bone Development , Dogs/growth & development , Osteogenesis , Animal Welfare , Animals , Bone and Bones/diagnostic imaging , Crime , Forensic Anthropology
4.
Eur J Histochem ; 60(3): 2701, 2016 Sep 15.
Article in English | MEDLINE | ID: mdl-27734993

ABSTRACT

Cartilage canals (CCs) are microscopic structures involved in secondary ossification centers (SOCs) development. The features of CCs were investigated in the humeral and femoral proximal epiphyses of small-sized newborn dogs (from premature to 28 days after birth) with histochemical and immunohistochemical approaches. Masson's Trichrome revealed a ring-shaped area around CCs, which changes in colour from green (immature collagen) to red (mature collagen) as ossification progresses; perichondrium staining always matched the ring colour. Safranin-O was always negative. Immunohistochemical analysis revealed immunopositivity for both collagen type I and V around the CCs; collagen type II was negative. CCs count showed a tendency to be higher in the humerus than in the femur. This work enlightened for the first time changes in composition of CCs surrounding matrix during SOCs development in dogs, paving the way to further investigations.


Subject(s)
Collagen Type I/biosynthesis , Collagen Type V/biosynthesis , Femur/cytology , Femur/metabolism , Growth Plate/cytology , Growth Plate/metabolism , Animals , Animals, Newborn , Dogs
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