1.
Biol Chem
; 382(8): 1171-7, 2001 Aug.
Article
in English
| MEDLINE
| ID: mdl-11592398
ABSTRACT
The partial purification and basic biochemical characterization of the RNase P holoenzymes of two species of methanogenic Archaea, Methanothermobacter thermoautotrophicus (previously Methanobacterium thermoautotrophicum strain deltaH) and Methanococcus jannaschii, are described. The properties of these enzymes, particularly buoyant density in Cs2SO4 and recent information about the subunit composition of the archaeal enzymes, suggest that RNase P enzymes in Archaea are much more alike than earlier studies in Sulfolobus acidocaldarius and Haloferax volcanii suggested.
Subject(s)
Endoribonucleases/chemistry , Endoribonucleases/isolation & purification , Methanobacteriaceae/enzymology , Methanococcus/enzymology , RNA, Catalytic/chemistry , RNA, Catalytic/isolation & purification , Centrifugation, Density Gradient , Endoribonucleases/metabolism , Molecular Weight , RNA, Catalytic/metabolism , Ribonuclease P
2.
AORN J
; 52(5): 987-91, 1990 Nov.
Article
in English
| MEDLINE
| ID: mdl-2264743