Real-world estimate of the value of one metabolic equivalent in a population of patients planning major surgery.

BACKGROUND: One metabolic equivalent (MET) is equal to resting oxygen consumption. The average value for one MET in humans is widely quoted as 3.5 mL/kg/min. However, this value was derived from a single male participant at the end of the 19th century and has become canonical. Several small studies have identified varied estimates of one MET from widely varying populations. The ability of a patient to complete 4 MET (or 14 mL/kg/min) is considered an indicator of their fitness to proceed to surgery. AIMS: To define a typical value of one MET from a real-world patient population, as well as determine factors that influenced the value. METHODS: A database of cardiopulmonary exercise testing (CPET) was interrogated to find a total of 1847 adult patients who had undergone CPET testing in the previous 10 years. From this database, estimates of oxygen consumption (VO2 ) at rest and at the anaerobic threshold and several other variables were obtained. The influence of age, body mass index (BMI), sex and the use of beta-blockers was tested. RESULTS: The median resting VO2 at rest was 3.6 mL/kg/min (interquartile range (IQR): 3.0-4.2). Neither sex, age >65 years or the use of beta-blockers produced a significant difference in resting VO2 , while those with a BMI >25 kg/m2 had a significantly lower VO2 at rest (3.4 mL/kg/min vs 4.0 mL/kg/min; P < 0.001). CONCLUSIONS: The estimate of 3.6 mL/kg/min for resting VO2 presented here is consistent with the previous literature, despite this being the first large study of its kind. This estimate can be safely used for pre-operative risk stratification.

Accumulation and effects of microplastic fibers in American lobster larvae (Homarus americanus).

The effects of microplastic fibers (MPF) on the survival, molting and oxygen consumption rates of larval (I-III) and post-larval (IV) stages of the American lobster, Homarus americanus, were quantified as a function of MPF concentration and food availability. Only the highest MPF concentration decreased early larval survival. MPF did not affect the timing or rate of molting across MPF treatments. While all larval and post-larval stages accumulated MPF under the cephalothorax carapace, stage II larvae and stage IV post-larvae showed the highest and lowest accumulation, respectively. MPF ingestion increased with larval stage and with MPF concentration; under starvation conditions, stage I larvae only ingested them at low MPF concentrations. Oxygen consumption rates were lower only in later larval stages when exposed to high MPF concentrations. Combined, our results indicate that MPF interactions and effects on American lobster larvae are dependent on larval stage, MPF concentration, and presence of food.

On the nature and origin of biological information: The curious case of RNA.

Biological information is most commonly thought of in terms of biology's Central Dogma where DNA is viewed as a linearized code used to synthesize proteins. Using DNA's chemical cousin, RNA, as a case study we consider how biological information operates outside the linear arrangement of its polymeric subunits. Much like individual pieces of a jigsaw puzzle, particular structures enable biomolecules to undergo precise molecular interactions with one another based on their respective shapes. By exploring the relationship between sequence and structure in RNA we argue that biological information finds its ultimate functional fulfillment in the three-dimensional structural arrangement of its atoms. We show how recurrent structural RNA motifs-operating at the tertiary level of a molecule-provide robust building blocks for the formation of new structural configurations and thereby convey the information required for emergent biological functions. We posit that these same RNA structures, guided by their respective thermodynamic stabilities, experience selective pressure to maintain particular three-dimensional architectures over and above pressures to maintain a particular sequence of nucleotides. Ultimately, this framework for understanding the nature of biological information provides a useful paradigm for understanding its origins and how biological information can result from chaotic prebiotic conditions.