ABSTRACT
SETTING: The search continues for a simple, rapid culture system for the isolation of Mycobacterium tuberculosis in low-income countries. Bio FM (BIO-RAD) medium is an enriched 7H9 medium optimised for the growth of mycobacteria and contains a chromogenic indicator. OBJECTIVE: To evaluate the detection rate and time to detection of mycobacteria using the Bio FM system in comparison with the Löwenstein-Jensen (LJ) medium method routinely used in our laboratory. MATERIALS AND METHODS: A total of 270 pulmonary and 178 extra-pulmonary samples were cultured in parallel on Bio FM and LJ media. The mycobacterial detection rate and time were compared. RESULTS: The mycobacteria detection rate on Bio FM and LJ were not significantly different (respectively 97.9% and 93.15%, P > 0.05). The growth of M. tuberculosis was faster on Bio FM (mean 12.42 days [3-41] vs. 20.7 [10-48] days for LJ, P < 10(-6)). CONCLUSION: In our study, the culture method on liquid Bio FM medium was faster, but the detection rate was not better than with solid LJ medium.
Subject(s)
Mycobacterium tuberculosis , Mycobacterium , Bacteriological Techniques , Culture Media , Culture Techniques , Humans , Laboratories , Mycobacterium/isolation & purification , Mycobacterium tuberculosis/isolation & purification , Poverty , Time FactorsABSTRACT
The clinical efficacy of chloroquine was assessed in the Primary Health Centre of Ankazobe, Central Highlands of Madagascar. This study shows an increase in the level of chloroquine resistance with the appearance of early treatment failures and RIII resistance. Furthermore, the prevalence of clinical treatment failures is approaching the level of 25%, at which WHO recommends a change of first-line drug.
Subject(s)
Antimalarials/therapeutic use , Chloroquine/therapeutic use , Malaria/drug therapy , Adolescent , Adult , Child , Child, Preschool , Drug Resistance , Female , Follow-Up Studies , Humans , Madagascar , Male , Middle Aged , Treatment FailureABSTRACT
We report a chronic case of pulmonary tuberculosis in a Malagasy citizen from Antsohihy (West of Madagascar), who was infected with a multi-drug resistant Mycobacterium bovis strain. This is the first case reported of the isolation of such a strain in Madagascar.
Subject(s)
Mycobacterium bovis , Tuberculosis, Multidrug-Resistant/drug therapy , Tuberculosis, Multidrug-Resistant/microbiology , Tuberculosis, Pulmonary/drug therapy , Tuberculosis, Pulmonary/microbiology , Amikacin/therapeutic use , Antitubercular Agents/therapeutic use , Capreomycin/therapeutic use , Chronic Disease , Ciprofloxacin/therapeutic use , Cough/microbiology , DNA, Bacterial/analysis , DNA, Bacterial/drug effects , DNA, Bacterial/genetics , Drug Therapy, Combination/therapeutic use , Ethionamide/therapeutic use , Humans , Kanamycin/therapeutic use , Madagascar/epidemiology , Male , Microbial Sensitivity Tests , Middle Aged , Mutation/drug effects , Mutation/genetics , Mycobacterium bovis/genetics , Ofloxacin/therapeutic use , Patient Compliance , Polymorphism, Restriction Fragment Length , Sputum/microbiology , Treatment Failure , Tuberculosis, Multidrug-Resistant/diagnosis , Tuberculosis, Pulmonary/diagnosisABSTRACT
"A case of pulmonary multiresistant tuberculosis (Mycobacterium bovis) in Madagascar"" : We report a chronic case of pulmonary tuberculosis in a Malagasy citizen from Antsohihy (West of Madagascar); who was infected with a multi-drug resistant Mycobacterium bovis strain. This is the first case reported of the isolation of such a strain in Madagascar."
Subject(s)
Drug Resistance , Mycobacterium bovisABSTRACT
A voluminous trichobezoar was discovered in an 11-year-old girl without major clinical implications. The diagnosis was made by endoscopy and surgery was performed without complications.
