ABSTRACT
New knowledge in biology led us to a better understanding of organization and functioning of living organisms. Today, re-evaluation of our concept of human biology is taking place. Theoretical analysis shows that taking into account the complexity of the organism and frequency of spontaneous mutations, it is impossible to explain the real time of organismal life. Therefore, besides extant systems, other repair systems must also exist. There are three "levels" at which a cell population withstands mutational pressure. First - intracellular (repair), second - intercellular (all forms of informational flows), and third - cellular replacement. Stem cells undertake regenerative functions following damage at the level of the tissue. They are also influenced by mutations, and for stem cells, it is most important that they preserve and support their full activity.
Subject(s)
Cell Communication , Gene Transfer, Horizontal , Stem Cells/physiology , Animals , CHO Cells , Cricetinae , Liver/embryology , Mice , MutationABSTRACT
A plant has been constructed for the free-flow electrophoresis. It permits carrying out electrophoretic separation both in a free flow and with the use of neutral grained fillers. Beta-galactosidase has been purified from the culture liquid of the Escherichia coli cell phage lysate. Its content in the purified material amounted to 81.2
Subject(s)
Electrophoresis/instrumentation , Galactosidases/isolation & purification , beta-Galactosidase/isolation & purification , Densitometry , Electrophoresis, Polyacrylamide Gel , Equipment Design , Escherichia coli/enzymology , Humans , Hydrogen-Ion Concentration , Infant, Newborn , Temperature , Ukraine , beta-Galactosidase/analysisABSTRACT
Damage of tissue culture cells (Hela, a human amnion, a primary rat liver culture) caused by the neodymium laser radiation was studied by time-lapse phase-contrast microfilming and electron microscopy. The tested cultures were shown to display different sensitivity and the degree of cell alteration within the same cultures varied considerably. A physical mechanism of cell damage is probably of thermal and shock-wave nature.
