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Prikl Biokhim Mikrobiol ; 46(1): 78-83, 2010.
Article in Russian | MEDLINE | ID: mdl-20198922

ABSTRACT

Hydroxylation activity of the mold fungi belonging to the orders Dothideales, Hypocreales, and Mucorales towards delta(5)-3beta-hydroxysteroids was studied. The fungi Bipolaris sorokiniana, Fusarium sp., and Rhizopus nigricans were able to introduce hydroxy group at position 7alpha; however, this ability was detected only at a low substrate load and with a low yield. A 7alpha-hydroxylase activity of the Curvularia lunata VKPM F-981 culture was shown for the first time. It was demonstrated that the studied strain was capable of stereo- and regioselective transformations of androstane 5-olefins at a load not less than 2 g/l. Conversion of pregnane steroids by this culture yielded both 7alpha and 11beta-hydroxy derivatives. The introduction of 7alpha-hydroxy group by this strain occurred concurrently with enzymatic hydrolysis of ester groups, which proceeded under mild conditions to give the corresponding alcohols in the cases of both 3-acetate of delta(5)-androstenes and mono- and triacetates of delta(5)-pregnenes.


Subject(s)
Alkenes/metabolism , Aryl Hydrocarbon Hydroxylases/metabolism , Ascomycota/enzymology , Fungal Proteins/metabolism , Hydroxysteroids/metabolism , Mucorales/enzymology , Steroid Hydroxylases/metabolism , Hydroxylation , Hypocreales/enzymology , Saccharomycetales/enzymology , Substrate Specificity
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