ABSTRACT
The problem of proper diagnosis, patient's status and disease progression is very actual for autoimmune diseases. Still the diagnosis of Sjogren's syndrome is more state of art than of science. Our preliminary investigations showed that serum fibronectin of elevated molecular weight may be probably considered as one of the diagnostic criteria in Sjogren's syndrome patients. The increase of molecular weight of FN chains from 220 kDa to 250 kDa derives from relative and absolute elevation in a content of N-acetylglucosamine in FN carbohydrate chains. The present results concern the presence of FN in cryoprecipitates and circulating immune complexes in Sjogren's syndrome patients and as it has been demonstrated this parameter may be used as a prognostic sign.
ABSTRACT
A new monoclonal antibody that recognizes a new antigen on the surface of mouse teratocarcinoma F9 stem cells has been described. This antigen is a glycolipid as demonstrated by inhibition of immunofluorescence by different monosaccharides, glycoproteins and glycolipid fraction of F9 cells as well as by chemical analysis. Immunofluorescent staining of in vitro cultivated preimplantation mouse embryos has demonstrated that this antigen is specific only of internal cell mass cells of late blastocyst.
Subject(s)
Antigens, Neoplasm/analysis , Blastocyst/immunology , Epitopes/analysis , Glycolipids/immunology , Membrane Lipids/immunology , Neoplastic Stem Cells/immunology , Teratoma/immunology , Animals , Antibodies, Monoclonal/analysis , Antibodies, Monoclonal/isolation & purification , Antigens/analysis , Antigens, Surface/analysis , Cells, Cultured , Embryonal Carcinoma Stem Cells , Fluorescent Antibody Technique , Glycolipids/analysis , Immunoglobulin G/analysis , Membrane Lipids/analysis , Mice , Mice, Inbred C3H , Tumor Cells, CulturedABSTRACT
Expression of gamma-glutamyltranspeptidase (GGT) in liver epithelial IAR 2 cells was studied after culturing on adhesive and non-adhesive substrates. IAR 2 cells are non-tumorigenic and do not express GGT under normalcy. Culturing these cells on a non-adhesive substrate dramatically retards the normal spreading up of these cells. Individual "islets" of the cells begin to express GGT activity tested by histochemistry. Biochemical testing of GGT activity in IAR 2 cells cultured on adhesive and non-adhesive substrates confirmed an assumption that maximal expression of GGT coincides in time with maximal morphological differences in the cells cultured on these substrates.
