1.
J Gen Microbiol
; 131(5): 993-9, 1985 May.
Article
in English
| MEDLINE
| ID: mdl-4020345
ABSTRACT
A gentle technique for preparing spheroplasts of Capnocytophaga ochracea strain 25 is described. Cells in the exponential phase were washed with 1.0 M-NaCl, agitated in 1.0 M-NaCl for 2 h at 30 degrees C and exposed to lysozyme in a Tris/salts buffer, pH 7.0. This procedure resulted in 98% spheroplast formation with complete removal of the peptidoglycan layer as detected by both phase-contrast and electron microscopy in combination with chemical analysis.
Subject(s)
Capnocytophaga/analysis , Cytophagaceae/analysis , Spheroplasts/isolation & purification , Capnocytophaga/ultrastructure , Methods , Microscopy, Electron
2.
Appl Environ Microbiol
; 36(4): 625-6, 1978 Oct.
Article
in English
| MEDLINE
| ID: mdl-708033
ABSTRACT
A method is described for the large-scale purification of the Bacillus thuringiensis protein crystal by zonal gradient centrifugation. NaBr gradients are employed in a Beckman J21-B centrifuge equipped with a JCF-Z rotor.
