Cytogenomic description of a Mexican cohort with differences in sex development.

BACKGROUND: Differences in Sex Development (DSD) is a heterogeneous group of congenital alterations that affect inner and/or outer primary sex characters. Although these conditions do not represent a mortality risk, they can have a severe psycho-emotional impact if not appropriately managed. The genetic changes that can give rise to DSD are diverse, from chromosomal alterations to single base variants involved in the sexual development network. Epidemiological studies about DSD indicate a global frequency of 1:4500-5500, which can increase to 1:200-300, including isolated anatomical defects. To our knowledge, this study is the first to describe epidemiological and genetic features of DSD in a cohort of Mexican patients of a third-level care hospital. METHODS: Descriptive and retrospective cross-sectional study that analyzed DSD patients from 2015 to 2021 attended a Paediatric Hospital from Mexico City. RESULTS: One hundred one patients diagnosed with DSD were registered and grouped into different entities according to the Chicago consensus statement and the diagnosis defined by the multidisciplinary group. Of the total, 54% of them belong to the chromosomal DSD classification, 16% belongs to 46, XX and 30% of them belongs to the 46, XY classification. CONCLUSION: The frequency for chromosomal DSDs was consistent with the literature; however, we found that DSD 46, XY is more frequent in our cohort, which may be due to the age of the patients captured, the characteristics of our study population, or other causes that depend on the sample size.

MAM-E: Mammographic Synthetic Image Generation with Diffusion Models.

Generative models are used as an alternative data augmentation technique to alleviate the data scarcity problem faced in the medical imaging field. Diffusion models have gathered special attention due to their innovative generation approach, the high quality of the generated images, and their relatively less complex training process compared with Generative Adversarial Networks. Still, the implementation of such models in the medical domain remains at an early stage. In this work, we propose exploring the use of diffusion models for the generation of high-quality, full-field digital mammograms using state-of-the-art conditional diffusion pipelines. Additionally, we propose using stable diffusion models for the inpainting of synthetic mass-like lesions on healthy mammograms. We introduce MAM-E, a pipeline of generative models for high-quality mammography synthesis controlled by a text prompt and capable of generating synthetic mass-like lesions on specific regions of the breast. Finally, we provide quantitative and qualitative assessment of the generated images and easy-to-use graphical user interfaces for mammography synthesis.

Development validation and evaluation of the knowledge test on cardiopulmonary resuscitation and correct use of the automated external defibrillator in Asunción 2023 / Desarrollo validación y evaluación de la prueba de conocimientos sobre reanimación cardiopulmonar y uso correcto del desfibrilador externo automático en Asunción 2023

Introduction: in out-of-hospital cardiac arrest, early and efficient intervention through cardiopulmonary resuscitation (CPR) maneuvers and the use of the automated external defibrillator (AED) are the cornerstone for survival. Instruments that improve education would increase the responsiveness of lay personnel. Objectives: to develop, validate and evaluate a knowledge test on cardiopulmonary resuscitation and correct use of the automated external defibrillator in Asunción during 2023. Methodology: observational cross-sectional study, non-probabilistic sample for convenience of lay personnel users of mass attendance centers in Asunción. An exclusive questionnaire was developed on knowledge in cardiopulmonary resuscitation and the use of the automated external defibrillator (KOR-AED) based on the chain of survival in out-of-hospital cardiac arrest. Content, construct, and internal consistency were validated using expert opinion, factor analysis and Cronbach's alpha. Results: a total of 200 lay people participated, mostly shopping mall customers, with a predominance of men (63.5 %) (127), between 25-29 years old 28.5% (57). 61.5 % (123) had a university education, 75.5 % (151) had not related to health, 52 % (104) had prior knowledge of CPR, but 81.5 % (163) did not. The test showed reliability and suitability for factor analysis (Cronbach's alpha 0.75, Kaiser-Meyers-Olkin; 0.78, Bartlett p<0.05). The questions covered the first three links in the chain of survival, with items of medium to high difficulty. Women performed significantly better (p=0.04). Conclusion: the KOR-AED test is a valid and reliable instrument to improve the education of the layperson in CPR and use of the AED based on the chain of survival.

Introducción: en la parada cardíaca extrahospitalaria la actuación precoz y eficiente mediante maniobras de reanimación cardiopulmonar (RCP) y uso del desfibrilador externo automático (DEA) constituyen la piedra angular para la supervivencia. Instrumentos que mejoren la educación aumentarían la respuesta del personal lego. Objetivos: desarrollar, validar y evaluar una prueba de conocimientos sobre reanimación cardiopulmonar y uso correcto del desfibrilador externo automático en Asunción durante el 2023. Metodología: estudio observacional corte transversal, muestro no probabilístico por conveniencia de personal lego usuarios de centros de concurrencia masiva de Asunción. Se desarrolló un cuestionario exclusivo sobre conocimientos en reanimación cardiopulmonar y uso del desfibrilador externo automático (COR-DEA) basado en la cadena de supervivencia en parada cardíaca extrahospitalaria. Se validó el contenido, el constructo y la consistencia interna mediante la opinión de experto, análisis de factores y el alfa de Cronbach. Resultados: participaron 200 legos mayormente clientes de shoppings, predominando hombres 63,5 % (127) entre 25-29 años 28,5 % (57). Con educación universitaria 61,5 % (123), no relacionados con la salud 75,5 % (151), con conocimientos previos en RCP 52 % (104), pero no en DEA 81,5 % (163). El test mostró fiabilidad y adecuación para análisis factorial (Alfa de Cronbach 0.75, Kaiser-Meyers-Olkin; 0.78, Bartlett p<0.05). Las preguntas abarcarón los tres primeros eslabones de la cadena de supervivencia, con ítems de dificultad media a alta. Las mujeres tuvieron significativamente mejor desempeñó (p=0.04). Conclusión: la prueba COR-DEA es un instrumento válido y fiable para mejorar la educación del lego en RCP y uso del DEA basado en la cadena de supervivencia.

Unraveling swine hepatitis E in the central region of Argentina through ELISA development and epidemiological insights.

Hepatitis E virus (HEV) is a public health concern globally, causing acute viral hepatitis in humans. Genotype-3 HEV (HEV-3), the most frequently genotype detected in South America, is zoonotic and the main reservoirs are the domestic pig and wild boar. Circulation of HEV-3 in Argentina has been confirmed in humans as well as in pig herds, wild boar and environmental waters. However, data are scarce mainly due to the inaccessibility of serological assays in this country. In order to provide insights in the epidemiology of HEV in swine in Argentina, we developed an indirect ELISA based on the native recombinant protein ORF2 and conducted a serological survey to determine the prevalence of seropositive swine in small-scale pig farms in the central region of Argentina. The method was evaluated in a panel of 157 serum samples, resulting in relative sensitivity of 98.6 % (95 % CI 95 %-100 %) and relative specificity of 97.7 % (95 % CI 94 %-100 %) compared to a commercial test. An almost perfect agreement was obtained between the two tests (Kappa index of 0.961). A survey on 294 samples from 49 small-scale farms resulted in a seropositivity rate of 54 %. Seropositive animals were found in 34 out of 49 (69.4 %) farms. Most of the farms (70.6 %) had over 50 % of seropositive animals. The wide spreading of HEV in the swine population of Tandil, Argentina, underscore the need to better understand the epidemiology of HEV in the region, enabling the implementation of targeted interventions to mitigate the impact of this virus on public health.

NGSEP 4: Efficient and accurate identification of orthogroups and whole-genome alignment.

Whole-genome alignment allows researchers to understand the genomic structure and variation among genomes. Approaches based on direct pairwise comparisons of DNA sequences require large computational capacities. As a consequence, pipelines combining tools for orthologous gene identification and synteny have been developed. In this manuscript, we present the latest functionalities implemented in NGSEP 4, to identify orthogroups and perform whole genome alignments. NGSEP implements functionalities for identification of clusters of homologus genes, synteny analysis and whole genome alignment. Our results showed that the NGSEP algorithm for orthogroups identification has competitive accuracy and efficiency in comparison to commonly used tools. The implementation also includes a visualization of the whole genome alignment based on synteny of the orthogroups that were identified, and a reconstruction of the pangenome based on frequencies of the orthogroups among the genomes. NGSEP 4 also includes a new graphical user interface based on the JavaFX technology. We expect that these new developments will be very useful for several studies in evolutionary biology and population genomics.

Treatment-related hemophagocytic lymphohistiocytosis due to atezolizumab: a case report and review of the literature.

BACKGROUND: Immune checkpoint inhibitors avoid inhibition of T-cell responses, upregulating antitumor immune response. Moreover, a dysregulation with hyperactive immune response can be caused, some of them underdiagnosed. Hemophagocytic lymphohistiocytosis is a rare and often fatal syndrome of uncontrolled and ineffective hyperinflammatory response that triggers an inflammatory cascade that can lead in many cases to death. CASE PRESENTATION: We report the case of a 67-year-old Caucasian man with stage IV lung adenocarcinoma who developed hemophagocytic lymphohistiocytosis after initiation of atezolizumab, an antagonist of programmed death-ligand 1. Even with early diagnosis and proper treatment, death occurs in approximately half of all cases reported. CONCLUSION: Key markers are needed to better identify patients at risk of developing severe immune-related adverse events. In addition to key markers, a higher degree of suspicion and early intervention are needed to improve outcomes in acquired hemophagocytic lymphohistiocytosis, especially with the increasingly and expanding use of immune activation.

Energetic Cost of Statistical Order-Degree Change in a Fermions' Set.

We discuss novel many-fermions thermodynamics' features. They refer to the energy cost associated to order-disorder changes. Our thermal quantum statistical scenario is controlled by suitable fermion-fermion interactions. We deal with two well-known quantum interactions that operate within an exactly solvable model. This model is able to adequately describe some aspects of fermion-dynamics, particularly level-crossings. We describe things via employment of Gibbs' canonical ensemble strictures. We show that judicious manipulation of the energy cost associated to statistical order (disorder) variations generates useful information-quantifiers. The underlying idea is that changes in the degree of order are intimately linked to level-crossings energetic costs.

Evaluation of the efficacy of polymeric antigen BLSOmp31 formulated in a new cage-like particle adjuvant (ISPA) administered by parenteral or mucosal routes against Brucella ovis in BALB/c mice.

Brucella ovis is an economically important cause of epididymitis in rams worldwide. Polymeric BLSOmp31 was previously identified as a protective immunogen against this pathogen. In this study, BLSOmp31 was formulated with a modified version of ISCOMATRIX adjuvant called ISPA (BLSOmp31/ISPA) and was administered in BALB/C by the subcutaneous and ocular route. The systemic and mucosal immune responses, the opsonic activity of antibodies and the protection conferred against B. ovis were evaluated. BLSOmp31+ISPA injected subcutaneously or by ocular route induced significantly higher IgG antibody levels with a mixed Th1/Th2 profile compared to non-immunized mice. IgA and IgG were detected in sera and nasal, tracheobronchial, vaginal secretions, tears and faeces, from SC immunized mice while in the group immunized by the ocular route a slight increase in both isotypes was mainly observed in all secretions, except in vaginal fluid. Opsonic antibodies stimulated binding and increased uptake of PHrodo™ Green-labelled B. ovis by neutrophils and monocytes. BLSOmp31 administered subcutaneously induced the highest levels of IFN-É£. The ocular immunization not only produced significant levels of this cytokine but also IL-4 compared to non-immunized mice. Both, subcutaneous and ocular routes of immunization, significantly protected against B. ovis infection. These results indicate that BLSOmp31/ISPA administered parenterally or by ocular route is a safe and effective vaccine against B. ovis in the murine model.

Thermal-Statistical Odd-Even Fermions' Staggering Effect and the Order-Disorder Disjunction.

We review thermal-statistical considerations on the odd-even staggering effect (OES) in fermions. There is a well known OES in nuclear binding energies at zero temperature. We discuss here a thermal OES (finite temperatures) that establishes links with the order-disorder disjunction. The present thermal considerations cannot be found in the nuclear literature.

Information-Theoretic Features of Many Fermion Systems: An Exploration Based on Exactly Solvable Models.

Finite quantum many fermion systems are essential for our current understanding of Nature. They are at the core of molecular, atomic, and nuclear physics. In recent years, the application of information and complexity measures to the study of diverse types of many-fermion systems has opened a line of research that elucidates new aspects of the structure and behavior of this class of physical systems. In this work we explore the main features of information and information-based complexity indicators in exactly soluble many-fermion models of the Lipkin kind. Models of this kind have been extremely useful in shedding light on the intricacies of quantum many body physics. Models of the Lipkin kind play, for finite systems, a role similar to the one played by the celebrated Hubbard model of solid state physics. We consider two many fermion systems and show how their differences can be best appreciated by recourse to information theoretic tools. We appeal to information measures as tools to compare the structural details of different fermion systems. We will discover that few fermion systems are endowed by a much larger complexity-degree than many fermion ones. The same happens with the coupling-constants strengths. Complexity augments as they decrease, without reaching zero. Also, the behavior of the two lowest lying energy states are crucial in evaluating the system's complexity.

Polymeric antigen BLSOmp31 formulated with class B CpG-ODN in a nanostructure (BLSOmp31/CpG-ODN/Coa-ASC16) administered by parenteral or mucosal routes confers protection against Brucella ovis in Balb/c mice.

Previously, we demonstrated that the chimera BLSOmp31 formulated in chitosan microspheres or Poloxamer407-Chitosan administered via the nasal and the ocular mucosa conferred partial protection in sheep against B. ovis. In this work, we tested a new delivery system for mucosal immunization with BLSOmp31 in the murine model to improve the efficacy of previously used formulations. First, we evaluated the protective efficacy against B. ovis induced by BLSOmp31 administered by the subcutaneous route using either BLSOmp31 alone, co-administered with immunostimulatory synthetic oligodeoxynucleotides containing unmethylated cytosine-guanine motifs (CpG-ODN) or with CpG-ODN in a nanostructure called Coa-ASC16 compared with BLSOmp31 emulsified in Incomplete Freund Adjuvant. Then, we evaluated the protection conferred by the best performing formulation (BLSOmp31/CpG-ODN/Coa-ASC16) administered by both subcutaneous and ocular routes. BLSOmp31/CpG-ODN/Coa-ASC16 injected subcutaneously did not induce higher IgG antibody levels compared to BLSOmp31 alone or BLSOmp31/CpG-ODN but it did stimulate a mixed immune Th1-Th2 response with the highest levels of IFN-É£ and conferred significant protection against the B. ovis challenge. Although ocular instillation of BLSOmp31/CpG-ODN/Coa-ASC16 showed a similar degree of protection compared to the parenteral route (3.66 and 3.60 logs of protection, respectively), it induced lower levels in serum of specific IgG (with mixed IgG1/IgG2a) and IgA antibodies and, less IFN-É£ and IL-4 than the subcutaneous route. No antibodies were detected in vaginal lavages or saliva. Fecal antigen-specific IgA was slightly higher in mice immunized with BLSOmp31/CpG-ODN/Coa-ASC16 subcutaneously compared with the ocular route. These results indicate that BLSOmp31/CpG-ODN/Coa-ASC16 was a safe and effective vaccine against B. ovis in mice.

Structural Statistical Quantifiers and Thermal Features of Quantum Systems.

This paper deals primarily with relatively novel thermal quantifiers called disequilibrium and statistical complexity, whose role is growing in different disciplines of physics and other sciences. These quantifiers are called L. Ruiz, Mancini, and Calvet (LMC) quantifiers, following the initials of the three authors who advanced them. We wish to establish information-theoretical bridges between LMC structural quantifiers and (1) Thermal Heisenberg uncertainties ΔxΔp (at temperature T); (2) A nuclear physics fermion model. Having achieved such purposes, we determine to what an extent our bridges can be extended to both the semi-classical and classical realms. In addition, we find a strict bound relating a special LMC structural quantifier to quantum uncertainties.

Cardiac arrest identified by a chest CT scan in a patient with normal telemetry findings.

Early recognition of cardiac arrest has been linked traditionally to clinical signs and telemetry findings. Few case reports have presented normal telemetry findings in patients with cardiac arrest where a contrast enhanced CT scan of the chest was able to identify the diagnosis. The early recognition of a cardiac arrest whether by telemetry monitoring or CT scan is important to improve the clinical outcomes. This case report presents a patient who was hypertensive and unresponsive upon arrival to the emergency department. A chest CT scan to rule out aortic dissection showed no contrast in the pulmonary arteries, aorta, and the rest of the heart chambers although normal telemetry findings were present. Resuscitation was initiated, and patient survived with poor neurological recovery.

Revisiting Entanglement within the Bohmian Approach to Quantum Mechanics.

We revisit the concept of entanglement within the Bohmian approach to quantum mechanics. Inspired by Bohmian dynamics, we introduce two partial measures for the amount of entanglement corresponding to a pure state of a pair of quantum particles. One of these measures is associated with the statistical correlations exhibited by the joint probability density of the two Bohmian particles in configuration space. The other partial measure corresponds to the correlations associated with the phase of the joint wave function, and describes the non-separability of the Bohmian velocity field. The sum of these two components is equal to the total entanglement of the joint quantum state, as measured by the linear entropy of the single-particle reduced density matrix.

Percepción que tienen los estudiantes de enfermería sobre el liderazgo estudiantil en una universidad nacional de la ciudad de Lima 2015 / Perception that have the nursing students about the student leadership at a National University in the city of Lima 2015

En enfermería, el liderazgo estudiantil esta concedido como parte de la formación académica profesional, que demuestra su actuar frente a las situaciones que la vida universitaria pone en su trayectoria hasta el momento de culminar sus estudios. Este liderazgo estudiantil es percibido por tres características: atribuciones de la elección del líder, expectativas del líder y su práctica de liderazgo. El presente estudio de investigación "Percepción que tienen los estudiantes de enfermería sobre el liderazgo estudiantil en una universidad nacional de lima, 2015", tiene como objetivo general determinar la percepción que tienen los estudiantes de enfermería hacia el liderazgo estudiantil en la Facultad de Medicina UNMSM. Es un estudio de tipo cuantitativo, método descriptivo, nivel aplicativo, de corte transversal. La población estuvo conformada por 284 estudiantes y la muestra por 75 estudiantes de enfermería de la Facultad de Medicina UNMSM. La técnica empleada fue la encuesta y el instrumento fue la escala tipo Likert modificada. Los principales hallazgos (53 por ciento) encontrados fueron que los estudiantes de enfermería tienen una percepción medianamente favorable sobre el liderazgo estudiantil en la facultad de Medicina UNMSM, evidenciado por los motivos que indujeron a la elección de los líderes estudiantiles, las expectativas que se tienen sobre el desarrollo de las funciones, y la práctica de liderazgo que desarrolla dentro del ejercicio mismo como líder estudiantil.

In nursing, student leadership is conceived as part of the professional academic training, demonstrating its act against situations that college life puts in their path until they complete their studies. The student leadership is perceived by three characteristics: powers of the election of the leader, the leader expectations and practice leadership. This research study "Perception of nursing students on student leadership in a national university of Lima, 2015," the general objective is to determine the perception of nursing students to the student leadership at the Faculty of Medicine UNMSM. It is a quantitative study, descriptive method, application level and cross section. The population consisted of 284 students and the sample by 75 nursing students of the Faculty of Medicine UNMSM. The technique used was the survey and the instrument was modified Likert scale. The main findings (53 per cent) were that nursing students have a fairly favorable perception of the student leadership at the Faculty of Medicine UNMSM, evidenced by the reasons which led to the election of student leaders, the expectations placed on the development of the functions, and practice leadership that develops within the same year as a student leader.

Malpractice and medical liability. European Guidelines on Methods of Ascertainment and Criteria of Evaluation.

The manuscript presents the European Guidelines on medico-legal Methods of Ascertainment and Criteria of Evaluation in cases of suspected subjective "Medical Responsibility and/or Liability" developed by an international working group under the patronage of the European Academy of Legal Medicine. It includes a step-by-step illustrated explanation of approved Flow Charts, articulated in 18 sequential steps and comprehensive of both Methods of Ascertainment and Evaluation Criteria.

Predictors of in-hospital mortality in patients with non-variceal upper gastrointestinal bleeding.

OBJECTIVE: to determine the independent predictors of in-hospital death of Hispanic patients with nonvariceal upper gastrointestinal bleeding (NVUGB). EXPERIMENTAL DESIGN: prospective and observational trial. PATIENTS: in a period between 2000 and 2009, all patients with NVUGB admitted to our hospital were studied. Demographical and clinical characteristics, endoscopic findings and laboratory tests were evaluated χ² and Mann-Whitney U analyses were per-formed for comparisons, and binary logistic regression was employed to identify independent predictors of in-hospital mortality. RESULTS: 1,067 patients were included, 65% male with a mean age of 58.8 years. Mean number of comorbidities per patient was 1.6 ± 0.76. The most frequent cause of bleeding were gastric and duodenal ulcers (55.4%); 278 patients (25.8%) received endoscopic treatment of which 69.1% had combined therapy. Rebleeding occurred in 36 patients (3.4%) of which 50% died. In-hospital mortality was 10.2%, of which only 3.1% was associated to bleeding. When comparing causes of death among patients with and without comorbidities, only hypovolemic shock was found significative (48.3 vs. 25%; p = 0.020). Binary logistic regression found that the number of comorbidities, Rockall scale score; serum albumin < 2.6 g/dL on admission; rebleeding and length of hospital stay were independent risk factors of in-hospital mortality. CONCLUSION: the number of comorbidities, the Rockall scales core, an albumin level < 2.6 g/dL, the presence of rebleeding and hospital stay were predictors of in-hospital mortality in patients with NVUGB.

Factores predictivos de mortalidad intrahospitalaria en pacientes con sangrado de tubo digestivo alto no variceal / Predictors of in-hospital mortality in patients with non-variceal upper gastrointestinal bleeding

Objetivo: determinar los factores de riesgo para mortalidad intrahospitalaria en pacientes hispanos con sangrado de tubo digestivo alto no variceal (STDANV). Diseño experimental: estudio prospectivo y observacional. Pacientes: del año 2000 al 2009 se estudiaron pacientes con STDANV. Se evaluaron variables demográficas y clínicas así como resultados de laboratorio y hallazgos endoscópicos. Se utilizaron análisis de χ² y U de Mann-Whitney para las comparaciones y de regresión logística binaria para la identificación de factores predictores de mortalidad. Resultados: se estudiaron 1.067 pacientes (65% hombres) con promedio de edad de 58,8 años. La media de comorbilidades por paciente fue 1,6 ± 0,76. La causa más frecuente de sangrado fueron las úlceras en estómago y duodeno (55,4%); 278 pacientes (25,8%) recibieron alguna forma de tratamiento endoscópico, siendo combinado en el 69,1%. Resangraron 36 pacientes (3,4%) de los cuales 50% fallecieron. La mortalidad intrahospitalaria fue del 10,2%, y el 3,1% se relacionó directamente al sangrado. Al comparar la mortalidad entre pacientes con y sin comorbilidades, solo la presencia de choque hipovolémico tuvo diferencias estadísticamente significativas (48,3 vs. 25%; p = 0,020). La regresión logística mostró que el número de comorbilidades, el puntaje de Rockall, la albúmina al ingreso < 2,6 g/dl, el resangrado y la estancia hospitalaria fueron factores de riesgo independientes para mortalidad. Conclusión: el número de comorbilidades, el puntaje de Ro - ckall, la presencia de albúmina sérica < 2,6 g/dl, el resangrado y la estancia hospitalaria son predictores de mortalidad intrahospitalaria en pacientes hispanos con STDANV(AU)

Objective: to determine the independent predictors of in-hospital death of Hispanic patients with nonvariceal upper gastrointestinal bleeding (NVUGB). Experimental design: prospective and observational trial. Patients: in a period between 2000 and 2009, all patients with NVUGB admitted to our hospital were studied. Demographical and clinical characteristics, endoscopic findings and laboratory tests were evaluated χ² and Mann-Whitney U analyses were performed for comparisons, and binary logistic regression was employed to identify independent predictors of in-hospital mortality. Results: 1,067 patients were included, 65% male with a mean age of 58.8 years. Mean number of comorbidities per patient was 1.6 ± 0.76. The most frequent cause of bleeding were gastric and duodenal ulcers (55.4%); 278 patients (25.8%) received endoscopic treatment of which 69.1% had combined therapy. Rebleeding occurred in 36 patients (3.4%) of which 50% died. Inhospital mortality was 10.2%, of which only 3.1% was associated to bleeding. When comparing causes of death among patients with and without comorbidities, only hypovolemic shock was found significative (48.3 vs. 25%; p = 0.020). Binary logistic regression found that the number of comorbidities, Rockall scale score; serum albumin < 2.6 g/dL on admission; rebleeding and length of hospital stay were independent risk factors of in-hospital mortality. Conclusion: the number of comorbidities, the Rockall scale score, an albumin level < 2.6 g/dL, the presence of rebleeding and hospital stay were predictors of in-hospital mortality in patients with NVUGB(AU)