ABSTRACT
The functioning of the propionate pathway of oxidation substrate metabolism in the norm and under vitamin B12 deficiency has been studied. This pathway has been shown to play an important role in oxidative processes occurring in normal organisms, for its inhibition in B12-deficient animals is associated with a reduction of respiration as well as with noticeable decreases in palmitoylcarnitine and succinate oxidation rates and oxidation phosphorylation coupling. Succinate, the end product of the propionate pathway, normalizes the respiration and restores the rate of palmitoylcarnitine oxidation in B12-deficient animals, which is suggestive of its crucial role in the propionate pathway. In vivo propionate inhibits, whereas methyl malonate stimulates palmitoylcarnitine respiration, however only in intact animals. In B12-deficient animals the sensitivity to these metabolites is decreased.
Subject(s)
Dicarboxylic Acids/metabolism , Fatty Acids/metabolism , Mitochondria, Liver/metabolism , Propionates/metabolism , Animals , Oxidation-Reduction , Rats , Rats, Wistar , Vitamin B 12 Deficiency/metabolismABSTRACT
Analysis of the clinical status of preterm newborns and the results of histochemical studies of the red cell catecholamine depositing function has led the authors to a conclusion that the Apgar score is reliable only in case of the newborn's critical condition. In slight and medium-severity asphyxia high red cell satiation with catecholamine granules was associated with a favorable outcome for the newborns. The authors claim that analysis of red cell catecholamine-depositing function is a sufficiently reliable diagnostic and prognostic criterion for the assessment of a preterm newborn's status.
Subject(s)
Catecholamines/blood , Infant, Premature, Diseases/diagnosis , Infant, Premature , Apgar Score , Asphyxia Neonatorum/diagnosis , Erythrocytes/chemistry , Humans , Infant, Newborn , PrognosisABSTRACT
Specific patterns of blood deoxygenation were studied using cholesterol-modified erythrocyte membranes by means of polarographic coulombometry in microlyzer. The rate of oxygen liberation was decreased in erythrocytes enriched with cholesterol as compared with controls. Effects of the membrane quantitative composition on its diffusion properties are discussed.
Subject(s)
Cholesterol/pharmacology , Erythrocyte Membrane/metabolism , Oxygen/blood , Diffusion , Erythrocyte Count , Erythrocyte Membrane/drug effects , Humans , In Vitro Techniques , KineticsABSTRACT
Effects of alcohol on tissue respiration were studied in mice in vivo. Alcohol decreased the efficiency of energy metabolism, affecting the working capacity. Possible mechanisms of impairment of tissue respiration in presence of alcohol are discussed. Effect of a single-day consumption of alcohol at high doses on functional characteristics of the vitally important tissues was studied in track and field athletes using the methods of auriculodiagnostics. Tissue dependent type of the alcohol intoxication as well as its long-term effects were shown.
Subject(s)
Ethanol/adverse effects , Galvanic Skin Response/drug effects , Oxygen Consumption/drug effects , Physical Endurance/drug effects , Adult , Animals , Energy Metabolism/drug effects , Humans , Mice , Time FactorsABSTRACT
Impairement in the process of tissue respiration at the subcellular level in growing animals with experimental pneumonia are described. Under the conditions of antibiotic therapy such impairements aggravated. A combined use of antibiotics with succinic and glutamic acids resulted in improvement of the tissue respiration and acceleration of reparation. Possible mechanisms of this phenomenon are discussed.
