1.
Chem Commun (Camb)
; 54(65): 9079-9082, 2018 Aug 09.
Article
in English
| MEDLINE
| ID: mdl-30058655
ABSTRACT
A new reagent for quantification of CgS in plant extracts using a generalized methodology suitable for recognition of homocysteine (Hcy) with luminescence ON response.
Subject(s)
Benzoxazines/pharmacology , Carbon-Oxygen Lyases/analysis , Fluorescent Dyes/pharmacology , Plant Extracts/analysis , Animals , Benzoxazines/chemical synthesis , Benzoxazines/chemistry , Cysteine/analysis , Fluorescent Dyes/chemical synthesis , Fluorescent Dyes/chemistry , HeLa Cells , Homocysteine/analysis , Humans , Kinetics , Limit of Detection , Microscopy, Confocal/methods , Microscopy, Fluorescence/methods , Nicotiana/chemistry , Zebrafish
2.
Chem Commun (Camb)
; 54(15): 1849-1852, 2018 Feb 15.
Article
in English
| MEDLINE
| ID: mdl-29384535
ABSTRACT
BODIPY derivative, SF-1, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles. SF-1 is compatible with 3D-SIM imaging of individual cells.
