ABSTRACT
OBJECTIVE: Estimation of microbiocenosis of lower and upper genital tract in different morphological forms of chronic endometritis and endometrial polyps. MATERIAL AND METHODS: Histological examination of endometrial aspirates and microview of the lower and upper genital tract in 164 women of reproductive age with different character of menstrual and reproductive history. RESULTS: The risk of endometrial colonization in, disturbance microecology of the vagina is 3.5 times higher than that, in patients with normosenosis (p<0.01, OP=3.5 [95%. CI 1.63-8.11]). CONCLUSION: The microbiological diagnosis can be considered as a component of comprehensive diagnostics necessary to choose the appropriate management of patients with CE and PE.
ABSTRACT
We analyzed sensitivity of 123 vaginal lactobacillus strains to antibacterial substances. All lactobacillus strains were sensitive to ampicillin, cefazolin, cefotaxime, and vancomycin, and insensitive to metronidazole, trimethoprim/sulfamethoxazole, and levofloxacin. Lactobacillus strains demonstrated different sensitivity to gentamycin, clindamycin, erythromycin, ciprofloxacin, and tetracycline. The phenomenon of preferential selective influence of antibacterial drugs on the composition of lactobacilli of the vaginal microbiota, in which some lactobacilli survive as part of the vaginal microbiota and have a selective advantage over other types of lactobacilli, should be taken into account during treatment of vaginal infections and dysbiosis.
Subject(s)
Anti-Bacterial Agents/pharmacology , Lactobacillus/drug effects , Microbiota/drug effects , Vagina/microbiology , Ciprofloxacin/pharmacology , Clindamycin/pharmacology , Erythromycin/pharmacology , Female , Gentamicins/pharmacology , Humans , Microbial Sensitivity Tests , Pregnancy , Tetracycline/pharmacologyABSTRACT
Coagulase-negative staphylococci (CoNS) are considered as a reservoir of mobile genetic elements and first of all of the staphylococcal cassette chromosome mec (SCCmec), defining staphylococci resistance to beta-lactams. Types II, IV, IVa, V, VII and VIII SCCmec were detected among 95 staphylococcal strains isolated in different regions of the Russian Federation. Subtypes C1a, C1b, C1c and C1 SCCmec were also identified (class B mec complex and two complexes of ccr1 and ccr2 genes recombinases). Some other cassette types carrying A, C1 and C2 classes of the mec complexes in combination with various recombinase genes were detected. The S.epidermidis isolates mainly formed cassettes carrying mec complex B, while the S. haemolyticus isolates had cassettes carrying classes C1 and C2 mec complex. Out of 9 isolates of S. hominis 5 isolates carried a new type cassette: class A mec complex in combination with the complex of the recombinase ccr1 genes. SCCmec was not identified in S. capitis and S. pasteuri. Their representatives carried either mec complex (1 isolate of S. pasteuri) or the recombinase complexes (2 isolates of S. capitis). The detected SCCmec variants in CoNS could be a source of emergence of new genetic lines of MRSA.
Subject(s)
Bacterial Proteins/genetics , Genomic Islands , Polymorphism, Genetic , Recombinases/genetics , Staphylococcus/genetics , beta-Lactam Resistance/genetics , Anti-Bacterial Agents/pharmacology , Bacterial Proteins/metabolism , Bacterial Typing Techniques , Chromosomes, Bacterial/chemistry , Gene Expression , Hospitals , Humans , Interspersed Repetitive Sequences , Microbial Sensitivity Tests , Molecular Sequence Data , Recombinases/metabolism , Russia , Staphylococcal Infections/drug therapy , Staphylococcal Infections/microbiology , Staphylococcus/drug effects , Staphylococcus/isolation & purification , Staphylococcus/pathogenicity , beta-Lactams/pharmacologyABSTRACT
R-plasmids from Enterobacteriaceae clinical strains, mainly Klebsiella and Serratia, isolated at different neonatal and children's hospitals of different cities of the former USSR for 10 years, were studied for their possible influence on the bacterial host phenotype. Hospital R-plasmids of stable inheritance persisted in hospitals from 2 to 7 years and were disseminated among strains of different genera (Klebsiella, Serratia, Enterobacter) and among different units. The data showed a possibility of long-term molecular rearrangements of R-plasmids in the hospital settings and an acquisition of genetic determinants encoding enterotoxin production. A novel R-plasmid encoding cytotoxicity to HEp-2 cells involved in two nosocomial outbreaks due to K. pneumoniae strains was reported. K. pneumoniae population heterogeneity was evaluated by using the plasmid parameters of strains. Their heterogeneity of a bacterial population was significantly lower during nosocomial outbreaks than in interepidemic periods.
